en تأثیر اکسیژن درمانی هیپرباریک در پاسخ التهابی یک مدل موشی آندومتریوز: یک مطالعه تجربی Reproductive Biology Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">پاتوژنز آندومتریوز که به وسیله ترشح واسطه&shy;های پیش&shy;التهابی نشان داده می&shy;شود یک التهاب است. وضعیت هیپوکسی می&shy;تواند این وضعیت را تحریک کند.</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بررسی اثر اکسیژن درمانی هایپرباریک (</span></span><span dir="LTR" style="font-size:10.0pt">HBOT</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) بر واکنش التهابی موش</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های آندومتریوز.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش&shy;ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">حیوانات در 3 گروه: اول) پیش‌آزمون، دوم) گروه پس‌آزمون دریافت‌کننده </span></span><span dir="LTR" style="font-size:10.0pt">HBOT</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و سوم) گروه پس‌آزمون بدون </span></span><span dir="LTR" style="font-size:10.0pt">HBOT</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">قرار گرفتند. همه گروه&shy;ها به مدت 15 روز تحت القای آپوپتوز به وسیله پیوند خارجی قرار گرفتند. </span></span><span dir="LTR" style="font-size:10.0pt">HBOT</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به مدت 30 دقیقه 3 بار در روز به مدت 10 روز داده شد. ارزیابی اثر </span></span><span dir="LTR" style="font-size:10.0pt">HBOT</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">با بررسی بافت آندومتر انجام شد. سطح التهاب با استفاده از سیستم نمره&shy;دهی نیمه کمی </span></span><span dir="LTR" style="font-size:10.0pt">Klopfleisch</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">(مقیاس شاخص </span></span><span dir="LTR" style="font-size:10.0pt">Remmele</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) ارزیابی شد، در حالی که بیان فاکتور هسته</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ای کاپا (</span></span><span dir="LTR" style="font-size:10.0pt">NF&kappa;B</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) بتا با رنگ</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">آمیزی ایمونوهیستوشیمی اندازه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">گیری شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج نشان داد که گروه اول بالاترین میزان التهاب (41/9 </span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&plusmn;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 99/1) را نسبت به گروه&shy;های پس&shy;آزمون (گروه دوم 60/1 </span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&plusmn;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 53/0؛ گروه سوم 42/2 </span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&plusmn;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 53/0) نشان داد. گروه‌های </span></span><span dir="LTR" style="font-size:10.0pt">HBOT</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کمترین سطح التهاب را در مقایسه با گروه غیر </span></span><span dir="LTR" style="font-size:10.0pt">HBOT</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">داشتند (020/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). نتایج نشان داد که </span></span><span dir="LTR" style="font-size:10.0pt">HBOT</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">درجه التهاب صفاقی ناشی از ضایعه آندومتر را در موش کاهش داد. بیان </span></span><span dir="LTR" style="font-size:10.0pt">NF&kappa;B</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در گروه‌های پس‌آزمون به‌طور معنی‌داری در مقایسه با گروه پیش‌آزمون (001/0 </span></span><span lang="FA" style="font-size:10.0pt">&ge; </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، با همبستگی قوی بین بیان </span></span><span dir="LTR" style="font-size:10.0pt">NF&kappa;B</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و سطح التهاب صفاقی (670/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">, r</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 001/0 </span></span><span lang="FA" style="font-size:10.0pt">&ge; </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) کاهش یافت.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه &shy;گیری:</span></span></b> <span dir="LTR" style="font-size:10.0pt">HBOT</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به طور قابل توجهی سطح التهاب ضایعه آندومتر را در موش کاهش داد که دربرگیرنده مسیر </span></span><span dir="LTR" style="font-size:10.0pt">NF&kappa;B</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">می</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">باشد.</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></span></span></span></span></span><br> <br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Endometriosis pathogenesis is related to the inflammation shown by the secretion of pro-inflammatory mediators. This hypoxia condition can stimulate this condition.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objective: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">To investigate the effect of hyperbaric oxygen therapy (HBOT) on the inflammation reaction of endometriosis-induced mice.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The animals were designated into 3 groups: I) the pre-test group, II) the post-test group receiving the HBOT, and III) the post-test group without HBOT. All groups were subjected to induction of endometriosis by xenotransplantation for 15 days. HBOT was given 30 min 3 times a day for 10 days. The evaluation of the HBOT effect was conducted by examining the endometrial tissue. The inflammation level was evaluated using the Klopfleisch semiquantitative scoring system (index remmele scale), whilst the expression of nuclear factor kappa (NF&kappa;B) beta was measured by immunohistochemical staining.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The results showed that group I demonstrated the highest level of inflammation degree (9.41 &plusmn; 1.99) compared to the post-test groups (group II: 1.60 &plusmn; 0.53; group III: 2.42 &plusmn; 0.53). The HBOT-groups was found to have the lowest inflammation level compared to the non-HBOT group (p = 0.020). The results demonstrated that HBOT lowered the peritoneal inflammation degree caused by the endometrial lesion in mice. NF&kappa;B expression on the post-test groups was significantly decreased, compared to the pre-test group (p &le; 0.001), with a strong correlation between the NF&kappa;B expression and the peritoneal inflammation level (p &le; 0.001, r = 0.670). </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> HBOT significantly reduced the inflammation level on the endometrial lesion in mice, involving the NF&kappa;B pathway. </span></span></span></span></span></div> آندومتریوز, اکسیژن­رسانی هایپرباریک, التهاب, هیپوکسی, موش. Endometriosis, Hyperbaric oxygenation, Inflammation, Hypoxia, Mouse. 347 356 http://ijrm.ir/browse.php?a_code=A-10-1659-1&slc_lang=en&sid=1 Dedy Syahrizal dedysyahrizal@unsyiah.ac.id 0000-0002-1908-3155 Yes Department of Biochemistry, Medical Faculty, Universitas Syiah Kuala, Banda Aceh, Indonesia. Cut Mustika drcutmustika@gmail.com 0000-0003-4814-1753 No Department of Public Health, Medical Faculty, Universitas Syiah Kuala, Banda Aceh, Indonesia. Nanda Ayu Puspita nandaayu@yahoo.com 0000-0002-7508-948x No Department of Biochemistry, Medical Faculty, Universitas Syiah Kuala, Banda Aceh, Indonesia. Mohammad Guritno Suryokusumo mguritno@yahoo.com 0000-0002-8546-7341 No Medical Hyperbaric Study Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia. Hendy Hendarto hndhendy@yahoo.com 0000-0001-5551-2028 No Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.