en تأثیر تغییرات هورمونی به تمامیت DNA اسپرم در بیماران الیگو آستنو تراتواسپرمیا: یک مطالعه مورد-شاهدی Reproductive Andrology Original Article Original Article <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تغییرات هورمونی یکی از فاکتورهای مهم دخیل در الیگوآستنوتراتواسپرمیاست. </span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف از این مطالعه ارزیابی اثرات هورمون&shy;های </span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt">,LH</span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:"> ,</span></span><span dir="LTR" style="font-size:10.0pt">FSH</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تستوسترون</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">,</span></span> <span dir="LTR" style="font-size:10.0pt">TSH</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، پرولاکتین و </span></span><span dir="LTR" style="font-size:10.0pt">AMH</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در میزان شکستگی </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> اسپرم در افراد نرمواسپرمیا در مقایسه با افراد الیگوآستنوتراتواسپرمیا به منظور طراحی یک الگوریتم بالینی برای مطالعه جامع ناباروری با فاکتور مردانه می</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">باشد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش&shy;ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نمونه سمن از 60 بیمار مراجعه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کننده به کلینیک ناباروری جمع</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">آوری و سپس تست تانل برای ارزیابی میزان شکستگی </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> بروی این نمونه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها انجام شد. بر اساس نتایج بررسی پارامترهای سمن و </span></span><span dir="LTR" style="font-size:10.0pt">DFI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> نمونه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها به 4 گروه مردان نرمواسپرمیا بدون افزایش </span></span><span dir="LTR" style="font-size:10.0pt">DFI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، نرمواسپرمیا با افزایش </span></span><span dir="LTR" style="font-size:10.0pt">DFI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، الیگو آستنو تراتواسپرمیا بدون افزایش </span></span><span dir="LTR" style="font-size:10.0pt">DFI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، الیگو آستنو تراتواسپرمیا با افزایش </span></span><span dir="LTR" style="font-size:10.0pt">DFI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> تقسیم&shy;بندی شدند. پارامترهای هورمونی با استفاده از تکنیک الایزا ارزیابی شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">سطح </span></span><span dir="LTR" style="font-size:10.0pt">FSH </span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">,</span></span><span dir="LTR" style="font-size:10.0pt">LH</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> ارتباط معناداری با افزایش </span></span><span dir="LTR" style="font-size:10.0pt">DFI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> نشان داد (01/0 </span></span><span lang="FA" style="font-size:10.0pt">&ge; </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). در حالیکه سطح تستوسترون و </span></span><span dir="LTR" style="font-size:10.0pt">TSH</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> ارتباط معناداری با غلظت اسپرم نشان می&shy;داد. سطح پرولاکتین و </span></span><span dir="LTR" style="font-size:10.0pt">AMH</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> به طور معناداری با غلظت اسپرم و میزان </span></span><span dir="LTR" style="font-size:10.0pt">DFI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> ارتباط داشت (01/0 </span></span><span lang="FA" style="font-size:10.0pt">&ge; </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه &shy;گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کاهش و افزایش میزان هورمون&shy;های سرم می&shy;تواند تأثیرات منفی روی پارامترهای سمن و میزان شکستگی </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> داشته باشد و منجر به ناباروری مردانه شدیدتری شود. هرچند ما اثرات این هورمون</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های اصلی در ناباروری مردانه در آسیب </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> اسپرم بررسی کردیم، مطالعات بیشتری جهت ارزیابی نقش این هورمون</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها بر میزان باروری و کیفیت جنین نیاز است.</span></span></span></span></span></span></span><br> &nbsp; <h1 style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:14pt"><span style="line-height:normal"><span new="" roman="" times=""><span style="font-size:12.0pt">Abstract</span></span></span></span></span></h1> <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:12pt"><span style="line-height:normal"><span new="" roman="" times=""><a name="_Hlk65272857"><b>Background: </b>Hormonal imbalance is one of the important etiological factors for Oligoasthenoteratospermias (OAT).</a></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span new="" roman="" times=""><a name="_Hlk65272871"><b>Objective</b></a><b>:</b> This study aimed to evaluate the effects of hormonal changes including prolactin, TSH, testosterone, luteinizing hormone, follicle-stimulating hormone and anti-Mullerian hormone on sperm DNA fragmentation in normal men compared with OAT to design a clinical algorithm for the comprehensive study of male factor infertilities.</span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span new="" roman="" times=""><a name="_Hlk65272887"><b>Materials and Methods:</b></a><b> </b>We consecutively selected 60 candidates referred to the infertility clinic to collect the semen and blood samples. Then, a terminal deoxynucleotidyl transferase dUTP nick end labeling test was performed to evaluate the sperm DNA fragmentation index (DFI). After semen analysis and DFI checking, they were classified into 4 groups consisting of normospermia and OAT men each with or without increased DFI. Hormone parameters were analyzed using enzyme-linked immunoassay.</span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span new="" roman="" times=""><b>Results: </b>Follicle-stimulating hormone and luteinizing hormone levels showed positive correlations with DFI in a significant way (p &le; 0.01), while testosterone and thyroid-stimulating hormone were associated with sperm concentration. Prolactin and anti-Mullerian hormone levels significantly correlated (p &le; 0.01) with sperm concentration and DFI value simultaneously.</span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span new="" roman="" times=""><b>Conclusion:</b> Decreased and increased levels of serum hormones could adversely affect semen profile and sperm DNA integrity which lead to severe male infertility. Although we investigated the effects of the main hormones related to male infertility on DNA damage, the role of these hormones on the fertilization rate and embryo quality needs to be evaluated in further studies.</span></span></span><br> &nbsp;</span></div> شکست DNA اسپرم, الیگواسپرمی, استنواسپرمی, تراتواسپرمی و هورمون ها. DNA fragmentation, Oligospermia, Asthenospermia, Teratospermia, Hormones 999 1006 http://ijrm.ir/browse.php?a_code=A-10-1776-1&slc_lang=en&sid=1 Zeinab Bahrami zn.bahrami@gmail.com 0000-0002-8026-9725 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Laleh IVF Clinic, Laleh Hospital, Tehran, Iran. Neda Daeifarshbaf Daeifarshbaf.n@gmail.com 0000-0003-2623-7543 No Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Infertility Center, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, Iran. Fatemehsadat Amjadi amjadi.bh@gmail.com 0000-0002-7489-7186 No Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Reza Aflatoonian R.aflatoonian@royaninstitute.org 0000-0002-5914-8662 Yes Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Laleh IVF Clinic, Laleh Hospital, Tehran, Iran.