en اثرات کیس پپتین بر کیفیت اسپرم های استخراج شده از بیضه موش سوری بالغ: یک مطالعه تجربی Reproductive Biology Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کیفیت اسپرم نقش مهمی در میزان موفقیت در تکنیک&shy;های کمک باروری ایفا می&shy;کند، افزودن برخی مواد می&shy;تواند با تأثیر مثبت بر اسپرم&shy;ها آن را بهبود بخشد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف از انجام این مطالعه بررسی اثر کیس&shy;پپتین بر روی ویژگی&shy;های حرکتی اسپرم، میزان فعالیت آنزیم </span></span><span dir="LTR" style="font-size:10.0pt">LDHC</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، واکنش آکروزومی و ظرفیت&shy;پذیری اسپرم&shy;های استخراج شده از بیضه موش سوری در شرایط آزمایشگاهی بود.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش &shy;ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نمونه اسپرم از بیضه 96 موش سوری از نژاد </span></span><span dir="LTR" style="font-size:10.0pt">BALB/c</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> به وزن 25 تا 30 گرم و سن 6 تا 8 هفته استخراج گردید. سپس به 4 بخش تقسیم گردید: 2 بخش مربوط به گروه کنترل و 2 بخش برای گروه تیمار شده با کیس</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">پپتین. هر کدام از این بخش</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها برای زمان</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های 15 و 30 دقیقه لحاظ گردیده بود. تحرک اسپرم و میزان فعالیت آنزیم </span></span><span dir="LTR" style="font-size:10.0pt">LDHC</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ارزیابی گردید و همچنین فراوانی اسپرم</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هایی که ظرفیت&shy;پذیری در آن&shy;ها صورت نگرفته، اسپرم&shy;های سالم، اسپرم&shy;هایی که واکنش آکروزومی را انجام داده&shy;اند به ترتیب توسط رنگ&shy;آمیزی&shy;های </span></span><span dir="LTR" style="font-size:10.0pt">WGA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt">PNA</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و </span></span><span dir="LTR" style="font-size:10.0pt">Con A</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ارزیابی گردید. اسپرم</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های رنگ شده از طریق فلوسیتومتری و میکروسکوپ فلورسنت آنالیز شدند.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج نشان داد کیس</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">پپتین باعث افزایش میزان تحرک اسپرم (04/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و فعالیت آنزیم &nbsp;</span></span><span dir="LTR" style="font-size:10.0pt">LDHC</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بعد از 15 دقیقه انکوبه شدن (04/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) می&shy;گردد، در حالی که در زمان مشابه بقیه فاکتورها از قبیل فراوانی اسپرم&shy;هایی که ظرفیت&shy;پذیری در آن&shy;ها رخ نداده، اسپرم&shy;های سالم و اسپرم&shy;هایی که واکنش آکروزومی را انجام داده اند (16/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) ، تحت تأثیر کیس&shy;پپتین قرار نگرفتند.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه&shy; گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">15 دقیقه انکوبه شدن با کیس&shy;پپتین می&shy;تواند باعث بهبود تحرک اسپرم و میزان فعالیت آنزیم </span></span><span dir="LTR" style="font-size:10.0pt">LDHC</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">گردد.</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></span></span></span></span></span><br> <br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Sperm quality has an important role in the success of assisted reproductive techniques, by adding some bioactive agents with a positive impact on sperms, it can be improved.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objective: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">This study aimed to evaluate the effects of kisspeptin on the sperm motility criteria, Lactate dehydrogenase-C</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> (</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">LDHC</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">)</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> activity, acrosomal reaction, and capacitation in the mouse testicular sperm in vitro.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Sperm samples were extracted from testes of 96 male Balb/C mice weighing 25-30 gr, aged 6-8 wk. Then, they were separated into 4 parts; 2 controls and 2 kisspeptin-treated aliquots; each one incubated for either 15 or 30 min. The sperm motility and the LDHC activity were evaluated, and also the frequency of the non-capacitated, intact, and acrosomal-reacted sperms were evaluated by staining with </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Wheat germ agglutinin</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">, </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Peanut agglutinin</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">, and </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Concanavalin A, </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">respectively. The stained sperms were analyzed by flow cytometry and fluorescent microscope. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Our result showed that kisspeptin increased both the sperm motility</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> (p = 0.04)</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> and LDHC enzyme activity </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">(p = 0.04) </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">after 15 min of incubation. At the same time, it did not impact the frequency of the non-capacitated, intact and acrosomal-reacted sperms after incubation in the same </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">period (p = 0.16). </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">A 15 min period of incubation with kisspeptin could be applicable for evaluating sperm motility and LDH activity.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></div> بیضه, اسپرم, کیس­پپتین, لاکتات دهیدروژناز, آکروزوم. Testis, Spermatozoa, Kisspeptin, Lactate dehydrogenase, Acrosome. 307 318 http://ijrm.ir/browse.php?a_code=A-10-1732-1&slc_lang=en&sid=1 Masoumeh Akmali akmali34@yahoo.com 0000-0003-2851-0312 No Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Roghayeh Yalmeh r.yalmeh.93@gmail.com 0000-0002-6277-2415 No Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Tahereh Talaei-Khozani talaeit@sums.ac.ir 0000-0002-8425-8871 No Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Fatemeh Karimi karimi_fa@sums.ac.ir 0000-0001-6805-277X No Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Elham Aliabadi aliabade@sums.ac.ir 0000-0002-5756-6166 Yes Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.