en ارزیابی سطح متیلن تتراهیدروفولات ردوکتاز و سطح اس-آدنوزیل متیونین در مردان ناباروری: یک مطالعه مورد-شاهدی Fertility & Infertility Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">آنزیم متیلن تتراهیدروفولات ردوکتاز (</span></span><span dir="LTR" style="font-size:10.0pt">MTHFR</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) نقشی اساسی در تنظیم تعادل فولات، تبدیل هموسیستئین به متیونین و تولید اس- آدنوزیل متیونین (</span></span><span dir="LTR" style="font-size:10.0pt">SAM</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) دارد که در روند متیلاسیون نقش ایفا می</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کند.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این تحقیق با هدف تعیین فعالیت </span></span><span dir="LTR" style="font-size:10.0pt">MTHFR</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و سطح </span></span><span dir="LTR" style="font-size:10.0pt">SAM</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در مردان مبتلا به نورموسپرمیا و الیگوزواسپرمی انجام شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش &shy;ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تعداد 30 مرد الیگوزواسپرمی غیر&shy;انسدادی و 30 مرد نرموسپرمی به عنوان گروه شاهد در این مطالعه مورد -شاهدی بود وارد شدند. آنالیز مایع سمن بر اساس معیارهای سازمان بهداشت جهانی صورت گرفت. سمن همه افراد پس از سه روز پرهیز رابطه جنسی جمع&shy;آوری گردید. اسپرم&shy;ها با تست اسپرم ارزیابی شدند و فعالیت </span></span><span dir="LTR" style="font-size:10.0pt">MTHFR</span><span style="font-size:10.0pt"><span b="" mitra="" style="font-family:"> بطور شیمیایی و </span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">میزان </span></span><span dir="LTR" style="font-size:10.0pt">SAM</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">توسط کیت الایزا اندازه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">گیری شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">دو گروه از نظر مورفولوژی اسپرم (02/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، غلظت (02/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و تحرک (03/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) تفاوت معنی&shy;داری داشتند. فعالیت </span></span><span dir="LTR" style="font-size:10.0pt">MTHFR</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در گروه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های نرموسپرمی و الیگوزواسپرمی تفاوت معنی</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">داری داشت (01/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). سطح </span></span><span dir="LTR" style="font-size:10.0pt">SAM</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در مایع سمن مردان الیگوزواسپرمی از نظر آماری کمتر از مردان نرموسپرمیک بود (03/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). همچنین، ارتباط مثبتی بین فعالیت آنزیم </span></span><span dir="LTR" style="font-size:10.0pt">MTHFR</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و سطح </span></span><span dir="LTR" style="font-size:10.0pt">SAM</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در گروه نرموسپرمیا (02/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، 67/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">&beta;</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و گروه اولیگوزواسپرمی (03/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، 54/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">&beta;</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) وجود داشت.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه &shy;گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">فعالیت </span></span><span dir="LTR" style="font-size:10.0pt">MTHFR</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و غلظت </span></span><span dir="LTR" style="font-size:10.0pt">SAM</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بطور معنی</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">داری در مردان اولیگوزواسپرمی کمتر بود. به نظر می&shy;رسد که آنها می&shy;توانند بر غلظت اسپرم، مورفولوژی و تحرک تأثیر بگذارند.</span></span><span lang="FA" style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Methylenetetrahydrofolate reductase enzyme (MTHFR) plays a key role in regulating folate balance, converting homocysteine to methionine, and producing s-adenosylmethionine (SAM) that plays a role in the methylation process. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objective: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">This study aimed to determine MTHFR activity and SAM level in men with normozoospermia and oligozoospermia. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> 30 oligozoospermic and 30 normozoospermic men as controls were enrolled in this case-control study. Semen analysis was conducted according to the world health organization criteria. All semen samples were collected after 3-5 days of sexual abstinence. The sperms were evaluated by sperm test video software. All subjects SAM level was measured by enzyme-linked immunosorbent assay kit, and MTHFR were measured manually. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> 2 groups had a significant difference in sperm morphology (p = 0.02), concentration (p = 0.02)</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> and motility (p = 0.03)</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">. The MTHFR activity in normozoospermic and oligozoospermic groups had significantly differences (p = 0.01). The level of SAM in the semen of oligozoospermic men was statistically lower than normozoospermic men (p = 0.03). Also, there was a positive association between MTHFR enzyme activity and SAM level in the normozoospermia group (p<i> </i>= 0.02, &beta; = 0.67) and oligozoospermia group (p<i> </i>= 0.03, &beta; = 0.54).</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> MTHFR activity and SAM concentration were statistically lower in oligozoospermia men. It seems they can affect sperm concentration, morphology, and motility. </span></span></span></span></span></span></div> متیلن تتراهیدروفولات ردوکتاز, اس- آدنوزیل متیونین, نرمواسپرمی, الیگواسپرمی, اسید فولیک. Methylenetetrahydrofolate reductase, S-adenosylmethionine, Normozoospermia, Oligozoospermia, Folic acid. 299 306 http://ijrm.ir/browse.php?a_code=A-10-1814-1&slc_lang=en&sid=1 Khadijeh Baranizadeh baranyzadeh@gmail.com 0000-0002-0566-5162 No Department of Clinical Biochemistry, Medicine School, Hamadan University of Medical Sciences, Hamadan, Iran. Maryam Bahmanzadeh bahmanzadeh12@yahoo.com 0000-0002-6585-9444 No Department of Anatomical Sciences, School of Medicine, Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. Heidar Tavilani tavilani@gmail.com 0000-0002-7243-660X No Department of Clinical Biochemistry, Medicine School, Hamadan University of Medical Sciences, Hamadan, Iran. Tayebeh Ghiasvand t_ghyasvand@yahoo.com 0000-0001-9797-436X No Department of Clinical Biochemistry, Medicine School, Hamadan University of Medical Sciences, Hamadan, Iran. Iraj Amiri amiri44@yahoo.com 0000-0003-4587-736X No Department of Anatomical Sciences, School of Medicine, Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. Mahnaz Yavangi m_yavangui@yahoo.com 0000-0003-2278-7644 No Department of Obstetrics and Gynecology, School of Medicine, Endometrium and Endometriosis Research Center, Fatemieh Hospital, Hamadan University of Medical Sciences, Hamadan, Iran. Gholamreza Shafiee g.r_shafiee@yahoo.com 0000-0003-2183-5122 Yes Department of Clinical Biochemistry, Medicine School, Nutrition Health Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.