International Journal of Reproductive BioMedicine

en Chronic scrotal heat stress causes testicular interstitial inflammation and fibrosis: An experimental study in mice Cellular and Molecular Biology of Reproduction Original Article Original Article <div style="text-align: justify;">Background: Chronic heat stress is a risk factor that adversely affects the reproduction system. Inflammation and fibrosis are 2 important response processes to damaged tissues. Objective: This study investigates the association of chronic scrotal heat stress with testicular interstitial inflammation and fibrosis in mice. Materials and Methods: For all experiments, 8-10 wk old male Swiss mice (Mus musculus) (20-23 gr) were divided into 3 groups (n = 10/each). The heat stress groups were submerged in a water bath at 37°C and 40°C, while the control group was treated at 25°C. The testicular tissues were performed hematoxylin and eosin staining, picro sirius red staining, and immunohistochemistry for intercellular adhesion molecule-1, fibroblast-specific protein 1, F4/80, collagen I, and Ki-67 staining to determine the testicular interstitial inflammation and fibrosis. Results: Chronic scrotal heat stress impairs spermatogenesis and reverses testicular histological structure. Heat stress significantly induced increased interstitial cell proliferation and upregulation of intercellular adhesion molecule-1 expression in the interstitial testicular tissue. In the interstitial testicular tissue, the number of F4/80-positive macrophages and the number of fibroblast-specific protein 1-positive fibroblasts were significantly increased in the heat-exposed groups compared to those in the control group. The heat exposed groups strongly increased extracellular matrix collagen accumulation in testicular interstitial tissues. Conclusion: Heat stress adversely affects the testicular structure and spermatogenesis, causes inflammation, and progresses to testicular interstitial fibrosis.</div> Heat stress, Testicular, Inflammation, Fibrosis. 569 580 http://ijrm.ir/browse.php?a_code=A-10-1820-1&slc_lang=en&sid=1 Tung Nguyen-Thanh nttung@huemed-univ.edu.vn 0000-0002-5215-383X Yes Faculty of Basic Science, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam. Institute of Biomedicine, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam. Phuoc Dang-Van Dangvanphuoc48@gmail.com 0000-0002-1606-4418 No Forensic Medicine Center in Thua Thien Hue Province, Hue, Vietnam. Phuc Dang-Ngoc phucdn@donga.edu.vn 0000-0003-2283-7461 No Faculty of Medicine, Dong A University, Da Nang, Vietnam. Won Kim kwon@jbnu.ac.kr 0000-0003-1068-1455 No Department of Internal Medicine, Jeonbuk National University Medical School, Jeonju, Republic of Korea. Tam Le-Minh leminhtam@huemed-univ.edu.vn 0000-0001-6225-3108 No Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam. Quoc-Huy Nguyen-Vu nvqhuy@huemed-univ.edu.vn 0000-0002-4744-7059 No Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam.