International Journal of Reproductive BioMedicine

en Author's Reply: "Low-dose bitter leaf improves sperm quality disrupted in immunosuppressed Wistar rats: An experimental study" Letter to Editor Letter to Editor Thank you for the observations made, clarifications required as well as the suggestions. We have gone through and our response is thus: 1) We understand that spermatogenesis cycle in rats lasts on an average for 56 days. We administered rats with prednisolone, then treated them with various doses of Vernonia amygdalina, and a comparison was drawn between the groups and the control. This comparison demonstrates the differences. We treated the rats for 21 days, which has shown to make a difference in their spermatogenesis parameters when compared with the untreated group.. Similar studies with shorter duration of administration have also shown difference in these sperm parameters, even with as low as 14 days of treatment when compared to controls (1–3). In our study, the rats were immunosuppressed with prednisolone, and then treated with low and high doses of Vernonia amygdalina extract, to observe for the different possible effects. These groupings were ideas conceived by the authors, which was part of the experimental design of the study. Furthermore, studies have shown various administrations of similar and higher doses being used (4, 5). According to the published data by Akah et al. and Nwanjo, acute toxicity test in rats gave an LD50 of 1122 mg/kg as well as 1265.22 ± 56 mg/kg (6, 7). quality,immunosuppressed 577 578 http://ijrm.ir/browse.php?a_code=A-10-474-2&slc_lang=en&sid=1 Risikat Eniola Kadir 0000-0002-0097-4783 No Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, PMB 1515, Nigeria. Abdulmumin Ibrahim ibrahim.am@unilorin.edu.ng 0000-0002-2839-2224 Yes Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, PMB 1515, Nigeria. Department of Human Biology, University of Cape Town, Cape Town, South Africa. Balkis Abimbola Ibrahim 0000-0001-8236-4796 No Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, PMB 1515, Nigeria. Sadiya Musa Gwadabe 0000-0003-0979-7338 No Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, PMB 1515, Nigeria. Rukayat Sulaiman-Jaji 0000-0003-4989-3922 No Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, PMB 1515, Nigeria. Munirat Foyeke Adigun 0000-0002-6174-309X No Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, PMB 1515, Nigeria. Adeoye Oyetunji Oyewopo 0000-0001-7217-098X No Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, PMB 1515, Nigeria.