en اثر ضد باروری عصاره خام Aerva lanata در فرزندان نر رت های حامله: یک مطالعه تجربی Reproductive Biology Original Article Original Article <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><i><span dir="LTR" style="font-size:10.0pt">Aerva lanata</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، گیاهی است که به عنوان غذا استفاده می</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">شود و همچنین با عنوان تقویتی توسط زنان باردار برای تسکین درد معده و جلوگیری از سقط جنین مصرف می</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">شود. این گیاه علاوه بر سایر خواص مشخصه، دارای فعالیت</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های ضد باروری و ضد لانه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">گزینی است.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه به بررسی سمیت بر روی بیضه فرزندان رت&shy;های تیمار شده با عصاره آبی خام </span></span><i><span dir="LTR" style="font-size:10.0pt">Aerva lanata</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> می&shy;پردازد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش &shy;ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">25 سر موش صحرایی باردار ویستار با وزن 180 تا 240 گرم به طور تصادفی در 5 گروه (هر کدام 5 سر) قرار گرفتند. گروه </span></span><span dir="LTR" style="font-size:10.0pt">A</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به عنوان کنترل انتخاب شد. گروه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های </span></span><span dir="LTR" style="font-size:10.0pt">B</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt">C</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt">D</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">E</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> به ترتیب 200، 400، 800 و 1000 میلی</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">گرم به ازای هر کیلوگرم وزن بدن عصاره </span></span><i><span dir="LTR" style="font-size:10.0pt">Aerva lanata</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> را از روز 12 تا 19 بارداری دریافت کردند. نوزادان (به دنیا آمده از رت&shy;ها) وزن شدند، تحت نظر گرفته شدند و 6 هفته پس از زایمان قربانی شدند. بیضه&shy;های توله&shy;های نر برای انجام مراحل بافت&shy;شناسی به دست آمد و بافت&shy;شناسی بیضه مورد بررسی قرار گرفت.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هیچ ناهنجاری فاحشی در گروه‌های مورد آزمایش مشاهده نشد، تعداد توله‌ها به‌طور معنی‌داری در گروه </span></span><span dir="LTR" style="font-size:10.0pt">E</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> کاهش یافت (01/0</span></span><span lang="FA" style="font-size:10.0pt"> = </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، آنالیز وزن نوزادان کاهش معنی‌داری را در گروه‌های </span></span><span dir="LTR" style="font-size:10.0pt">C</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">E</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> نشان داد (به ترتیب 04/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و 02/0 </span></span><span lang="FA" style="font-size:10.0pt">=</span><span dir="LTR" style="font-size:10.0pt">p</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). میانگین وزن بیضه&shy;های توله&shy;ها در گروه&shy;های </span></span><span dir="LTR" style="font-size:10.0pt">B</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt">C</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt">D</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">E</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (به ترتیب 03/0، 03/0، 01/0 و 001/0 </span></span><span lang="FA" style="font-size:10.0pt">></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) به طور قابل توجهی در مقایسه با گروه کنترل کاهش یافته بود. از نظر بافت&shy;شناسی، بافت&shy;های بیضه توله&shy;های تیمار شده درجات مختلفی از اختلال و به هم ریختگی ترتیب سلولی اپیتلیوم ژرمینال را به شیوه&shy;ای وابسته به دوز در مقایسه با گروه کنترل نشان دادند.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه &shy;گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه سمیت بر روی بیضه و احتمالاً نقش ضد باروری </span></span><i><span dir="LTR" style="font-size:10.0pt">Aerva lanata</span></i> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">را در نوزادان رت&shy;ها نشان داد.</span></span></span></span></span></span></span> <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><strong><span style="font-size:12.0pt">Background:</span></strong><span style="font-size:12.0pt"> <i>Aerva lanata, </i>a herb used as food and also consumed as a tonic by pregnant women to relieve stomach pains and prevent miscarriage. In addition to other characterized properties, it possesses antifertility and anti-implantation activities.<i></i></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><strong><span style="font-size:12.0pt">Objective:</span></strong><span style="font-size:12.0pt"> This study investigates the testicular toxicity of the testes of offsprings of Dams treated with crude aqueous extract of <em>Aerva lanata</em>.</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><strong><span style="font-size:12.0pt">Materials and Methods:</span></strong><span style="font-size:12.0pt"> 25 pregnant Wistar rats (Dams) weighing 180-240 gr were randomly earmarked into 5 groups (n = 5/each). Group A served as control; groups B, C, D, and E received 200, 400, 800, and 1000 mg/kg body weight of <em>Aerva lanata</em> extract<em>,</em> respectively, beginning from 12^th to 19^th day of gestation. The pups (delivered of Dams) were weighed, observed, and sacrificed 6 wk post-parturition. The testes of the male pups were obtained for histological procedures the testis histology was examined.</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><strong><span style="font-size:12.0pt">Results:</span></strong><span style="font-size:12.0pt"> No gross malformation was observed in the treatment groups, the number of pups/litter was significantly reduced in group E (p<i> </i>= 0.01), pups weight analysis showed a significant reduction in groups C and E (p<i> </i>= 0.04, and 0.02 respectively), and the mean pup testes weight was significantly reduced in groups B, C, D, and E (p<i> </i>= 0.03, 0.03, 0.01, and</span><span style="font-size:12.0pt"> < 0.001 </span><span style="font-size:12.0pt">respectively) when compared with control. Histologically, the treated pup testes tissues showed varying degrees of disruption and distortion of the cellular arrangements of the germinal epithelium in a dose dependent manner compared to the control.</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><strong><span style="font-size:12.0pt">Conclusion:</span></strong><span style="font-size:12.0pt"> The study revealed a testicular toxicity and possibly antifertility role of <em>Aerva lanata</em> in dams&rsquo; pups.</span></span></span></span><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></div> تاج خروس, باروری, باروری نر, دستگاه تناسلی نر, گیاهان محلی. Amaranthaceae, Fertility, Male fertility, Male reproductive system, Local herbs. 237 244 http://ijrm.ir/browse.php?a_code=A-10-1898-1&slc_lang=en&sid=1 Raphael Eguono Uwejigho ruwejigho@unimed.edu.ng 0000-0003-4328-6769 Yes Department of Anatomy, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo State, Laje Campus, Ondo City, Ondo State, Nigeria. Kingsley Afoke Iteire aiteire@unimed.edu.ng 0000-0002-5786-2926 No Department of Anatomy, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo State, Laje Campus, Ondo City, Ondo State, Nigeria. Felix Udawmojo Enemali fenamali@unimed.edu.ng 0000-0001-9972-1382 No Department of Anatomy, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo State, Laje Campus, Ondo City, Ondo State, Nigeria.