en مقایسه پیامد بارداری حاصل از انتقال جنین در مرحله کلیواژ و بلاستوسیست در سیکل انتقال جنین فریز شده: یک مطالعه مقطعی Embryology Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه:</span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> لقاح آزمایشگاهی از بسیاری جهات پیشرفت کرده است و تکنیک&shy;های جدید چالش&shy;برانگیز هستند</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">.</span></span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> انتقال بلاستوسیست یک روش جایگزین برای انتقال جنین (</span></span><span dir="LTR" style="font-size:10.0pt">ET</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) برای بهبود نتایج لقاح آزمایشگاهی است.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مطالعه حاضر با هدف تعیین تأثیر بارداری&shy;های ناشی از</span></span> <span dir="LTR" style="font-size:10.0pt">ET</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در مرحله بلاستوسیست نسبت به مرحله کلیواژ در سیکل&shy;های فریز شده انجام شد تا روش بهتری برای کمک باروری انتخاب شود</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش&shy; ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه مقطعی بر روی 194 زن مراجعه‌کننده به پژوهشکده علوم تولیدمثل یزد در فاصله زمانی فروردین 1398 تا آذر 1399 انجام شد. زنان شرکت&shy;کننده کاندید سیکل فریز در دو مرحله بلاستوسیست یا کلیواژ بودند. این مطالعه نتایج حاملگی و جنین را در 2 گروه</span></span> <span dir="LTR" style="font-size:10.0pt">ET</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در بلاستوسیست یا کلیواژ مقایسه کرد</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج نشان داد که گروه مرحله بلاستوسیست نسبت به گروه مرحله کلیواژ دارای سطوح بالاتری از هورمون آنتی&shy;مولرین، تعداد تخمک، </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">عدد دو پرونوکلئوس</span></span><span dir="LTR" style="font-size:11.0pt">&shy;</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و تعداد جنین بود. فراوانی حاملگی&shy;های شیمیایی در گروه بلاستوسیست و کلیواژ به ترتیب 6/52% و 1/36% بود (021/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). همچنین فراوانی حاملگی&shy;های بالینی در گروه بلاستوسیست و کلیواژ به ترتیب 2/41% و 7/22% بود (001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). بین دو گروه در سقط، زایمان زودرس، چند&shy;قلو، پارگی زودرس پرده، دیابت حاملگی و پره&shy;اکلامپسی، حاملگی خارج رحمی، بستری نوزاد در بخش مراقبت‌های ویژه نوزادان و ناهنجاری‌های جنین تفاوت آماری معنی‌داری مشاهده نشد (05/0 </span></span><span lang="FA" style="font-size:10.0pt">< </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه&shy; گیری: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج نشان داد که انتقال در مرحله بلاستوسیست نسبت به مرحله کلیواژ با افزایش حاملگی شیمیایی و بالینی همراه است، در حالی که سایر پیامدهای بارداری در هر دو گروه یکسان بود</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">.</span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background:</span></span></b> <span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">In vitro fertilization has advanced in many ways, and new techniques are challenging</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">. Blastocyst transfer is an alternative method for embryo transfer (ET) to improve </span></span><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">in vitro fertilization </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">outcomes.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The present study was performed to determine the effect of pregnancies resulting from ET in the blastocyst stage compared to the cleavage stage in frozen cycles to select a better method of assisted reproduction.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> This cross-sectional study was conducted on 194 women who referred to the Yazd Reproductive Sciences Institute, Yazd, Iran, between April 2019 and December 2020. They had a frozen ET as either cleavage or blastocyst (n = 97/each group). The study compared the pregnancy and fetal outcomes in the 2 groups of ET at the cleavage and blastocyst stages.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The results showed that the blastocyst stage group had higher levels of anti-Mullerian hormone, ovule number, 2 pronuclear number, and embryo number than the cleavage stage group. The frequency of chemical pregnancies was 52.6% and 36.1% in blastocyst and cleavage group respectively (p = 0.02). Also, the frequency of clinical pregnancies was 41.2% and 22.7% in blastocyst and cleavage group respectively (p < 0.001). No statistically significant difference was observed between 2 groups in abortion, preterm delivery, multiple births, </span></span><a href="https://www.degruyter.com/document/doi/10.1515/jpm-2017-0027/html?lang=en" style="color:blue; text-decoration:underline"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">preterm premature rupture of membranes</span></span></a><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">, </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">gestational diabetes and preeclampsia, ectopic pregnancy, neonatal hospitalization in </span></span><a href="https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&ved=2ahUKEwjV9vbng-X6AhVPSfEDHXPAAKUQFnoECCYQAQ&url=http%3A%2F%2Fwww.academyofneonatalnursing.org%2Fprivate%2FJournalFiles%2FSepOct11CEArticles.pdf&usg=AOvVaw1BCwx28sB-VgQL92tt6-Lu" style="color:blue; text-decoration:underline"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="text-decoration:none"><span style="text-underline:none">Neonatal Intensive Care Unit</span></span></span></span></a><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">, and fetal abnormalities (p > 0.05).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The results showed that transmission in the blastocyst stage compared to the cleavage stage is associated with an increase in chemical and clinical pregnancy, while other pregnancy outcomes are the same in both groups.</span></span></span></span></span></div> بلاستوسیست, کلیواژ, انتقال جنین. Blastocyst, Ovum cleavage sstage, Embryo transfer. 929 936 http://ijrm.ir/browse.php?a_code=A-10-2002-1&slc_lang=en&sid=1 Zahra Parsafar sefidkamrang@yahoo.com 0000-0002-6976-328x No Department of Obstetrics and Gynecology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Razieh Dehghani-Firouzabadi irouzabadirzieh@gmail.com 0000-0002-3157-6518 Yes Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.