International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1401
2
1
gregorian
2022
5
1
20
5
online
1
fulltext
en
مقایسه پتانسیل زتا و ICSI فیزیولوژیکی (PICSI) در به دست آوردن اسپرم با یکپارچگی DNA بهتر: یک مطالعه مقطعی
Comparison of zeta potential and physiological intracytoplasmic sperm injection in obtaining sperms with a lower DNA fragmentation index: A cross-sectional study
Reproductive Andrology
Original Article
Original Article
<div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">شاخص تجزیه </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">اسپرم (</span></span><span dir="LTR" style="font-size:10.0pt">DFI</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) یکی از معیارهای سلامت باروری مردان است که بر نتایج تکنیک­های کمک باروری (</span></span><span dir="LTR" style="font-size:10.0pt">ART</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) تأثیر می­گذارد. به نظر می­رسد تلاش برای به دست آوردن اسپرم بالغ با کیفیت بالا ضروری است. تکنیک­های پیشرفته انتخاب اسپرم مانند تزریق فیزیولوژیکی اسپرم درون سیتوپلاسمی (</span></span><span dir="LTR" style="font-size:10.0pt">PICSI</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">)، پتانسیل زتا، میکروسیالی در این زمینه محبوبیت یافته است.</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">هدف از این مطالعه مقایسه اثر پتانسیل زتا و انتخاب اسپرم </span></span><span dir="LTR" style="font-size:10.0pt">PICSI</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">در بدست آوردن اسپرم با یکپارچگی </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">بهتر بود.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">مواد و روش ­ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">در این مطالعه مقطعی، 48 بیمار شرکت کردند که شریک مرد در نمونه انزال خود </span></span><span dir="LTR" style="font-size:10.0pt">DFI</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">اسپرم بالا داشتند و زن در سلامت باروری طبیعی قرار داشت. برای هر بیمار، نمونه مایع منی با پتانسیل زتا و تکنیک­های </span></span><span dir="LTR" style="font-size:10.0pt">PICSI</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">آماده­سازی شد، سپس </span></span><span dir="LTR" style="font-size:10.0pt">DFI</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">اسپرم منی شسته شده با آزمایش پراکندگی کروماتین اسپرم (</span></span><span dir="LTR" style="font-size:10.0pt">SCD</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) با نمونه­های زتا و </span></span><span dir="LTR" style="font-size:10.0pt">PICSI</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">مقایسه شد.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">داده­ها نشان داد که هر دو پتانسیل زتا و تکنیک </span></span><span dir="LTR" style="font-size:10.0pt">PICSI</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">باعث کاهش </span></span><span dir="LTR" style="font-size:10.0pt">DFI</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">اسپرم در مقایسه با نمونه مایع منی شد (001/0 </span></span><span lang="FA" style="font-size:10.0pt">≥ </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> برای هردو). علاوه بر این، تفاوت آماری معنی­داری در </span></span><span dir="LTR" style="font-size:10.0pt">DFI</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">اسپرم بین </span></span><span dir="LTR" style="font-size:10.0pt">PICSI</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">و نمونه بالقوه زتا وجود دارد (01/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">).</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتیجه­ گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">مطالعه حاضر نشان داد که هر دو پتانسیل زتا و </span></span><span dir="LTR" style="font-size:10.0pt">PICSI</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">می</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif"">­</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">توانند منجر به اسپرم با یکپارچگی </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">بهتر شوند. با این حال، به نظر می­رسد </span></span><span dir="LTR" style="font-size:10.0pt">PICSI</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">در این زمینه از پتانسیل زتا برتر است.</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></span></span></span></span></span><br>
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<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif"">Background: </span></span></b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif"">The sperm DNA fragmentation index (DFI) is one of the men's reproductive health criteria that affects assisted reproductive technique outcomes. Efforts in obtaining high-quality mature sperms seem to be necessary. Advanced sperm selection techniques (including physiological intracytoplasmic sperm injection [PICSI], zeta potential, microfluidic, etc.) have gained popularity in this regard.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif"">Objective:</span></span></b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""> The study aimed to compare the efficacy of zeta potential and PICSI sperm selection in obtaining sperms with better DNA integrity.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif"">Materials and Methods: </span></span></b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif"">In this cross-sectional study, 48 couples were enrolled where the male partner had increased sperm DFI in his ejaculated sample and the female was in normal reproductive health. For each male partner, the semen sample was processed with zeta potential and PICSI techniques, then the sperm DFI of neat semen was compared to zeta and PICSI samples by the sperm chromatin dispersion test.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif"">Results:</span></span></b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""> Data showed that both the zeta potential and PICSI technique decreased sperm DFI in comparison with the neat semen sample (p <u><</u> 0.001 for both). In addition, there was a statistically significant difference in sperm DFI between the PICSI and zeta potential samples (p < 0.01).</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,"sans-serif""><b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif"">Conclusion:</span></span></b> <span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif"">The current study showed that both zeta potential and PICSI could result in sperm with a lower DFI. However, PICSI seems to be superior to zeta potential in this regard. </span></span><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></div>
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PICSI, پتانسیل ZETA, اسید هیالورونیک, یکپارچگی DNA.
PICSI, ZETA potential, Hyaluronic acid, DNA integrity.
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http://ijrm.ir/browse.php?a_code=A-10-624-4&slc_lang=en&sid=1
Serajoddin
Vahidi
Vahidi.seraj@gmail.com