en پیامدهای بارداری و انتقال جنین تازه در مرحله کلیواژ و بلاستوسیست: یک مطالعه گذشته نگر مقطعی Assisted Reproductive Technologies Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">پیشرفت</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها در زمینه کشت سلولی موجب تغییراتی در روند انتقال جنین از مراحل اولیه کلیواژ به انتقال جنین در مرحله بلاستوسیست شده است.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مطالعه حاضر با هدف مقایسه اثرات انتقال جنین تازه در مرحله کلیواژ و بلاستوسیست بر پیامدهای بارداری انجام شده است.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش &shy;ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه مقطعی برروی 1422 بیمار مراجعه&shy;کننده به مرکز ناباروری ام&shy;البنین، دزفول، ایران طی سال&shy;های 2013 ژولای-دسامبر 2020 جهت انجام لقاح آزمایشگاهی/تزریق داخل سیتوپلاسمی اسپرم (</span></span><span dir="LTR" style="font-size:10.0pt">IVF/ICSI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و انتقال جنین تازه انجام شد. در مجموع 1246 پرونده بیماران واجد شرایط به چهار دسته دو تا پنج یا شش روزه تقسیم شدند. میزان حاملگی شیمیایی و بالینی، سقط جنین، حاملگی چندقلویی، بارداری در حال پیشرفت و تولد زنده مورد آنالیز قرار گرفتند.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">انتقال جنین تازه در 5/28% از بیماران در روز دوم، 8/45% در روز سوم، 3/15% در روز چهارم و 4/10% در روزهای پنجم یا ششم انجام شد. میزان کلی حاملگی بالینی و تولد زنده در مرحله کلیواژ 6/20% و 6/17% و در مرحله بلاستوسیست 17% و 14% به ترتیب برآورد شد. با این حال، تفاوت معنی&shy;داری در هر دو گروه مشاهده نشد. بعلاوه، ارتباطی بین سایر پیامدها از جمله میزان سقط جنین، حاملگی چندقلویی و حاملگی در حال پیشرفت مشاهده نشد (05/0 </span></span><span lang="FA" style="font-size:10.0pt">< </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). </span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه&shy; گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بر اساس نتایج، پیامدهای بارداری در انتقال جنین تازه در مرحله بلاستوسیست نسبت به انتقال جنین در مراحل مختلف کلیواژ برتری نشان نداد.</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Advances in cell culture media have led to a trend of embryo transfer from the early cleavage stages to blastocyst stage</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The study aims to compare the effects of fresh embryo transfer in cleavage and blastocyst stage on pregnancy outcomes. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> This cross-sectional study was conducted on 1422 cases referred to</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> the Umm-al-Banin Infertility Clinic Center, Dezful, Iran</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">,</span></span> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">between July 2013 and December 2020 who were candidates for</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> in vitro fertilization/ intracytoplasmic sperm injection </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">for fresh embryo transfer. A total of 1246 cases were divided into 4 categories on days 2-5 or 6. Chemical and clinical pregnancy, abortion, multifetal pregnancy, ongoing pregnancy, and live birth rates were </span></span><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">analyzed</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">. </span></span></span></span></span></span><br> <span style="line-height:normal"><span style="tab-stops:42.45pt"><span style="unicode-bidi:embed"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Fresh</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> embryo transfer was performed in 28.5% of the </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">cases</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> on the 2^nd day, </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">45.8%</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> on the 3^rd, </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">15.3% </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">on the 4^th day, and </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">10.4</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">% on days 5 or 6. The overall clinical pregnancy and live birth rates were estimated at 20.6% and 17.6% in the cleavage, and 17</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">% </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">and 14% in the blastocyst stages, respectively. However, no significant difference was observed in either group. In addition, there was no significant difference between groups in terms of the abortion, </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">multifetal pregnancy</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">, and </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">ongoing pregnancy rate </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">(</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">p ˃ 0.05</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">). </span></span></span></span></span><br> <span style="line-height:normal"><span style="tab-stops:42.45pt"><span style="unicode-bidi:embed"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> According to the results, the consequences of pregnancy in</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> fresh</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> embryo transfer at the blastocyst stage were not superior to embryo transfer at </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">different stages of</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> the cleavage</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> process</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">. </span></span></span></span></span></div> انتقال جنین, کلیواژ, بلاستوسیست, پیامدهای بارداری. Embryo transfer, Cleavage, Blastocyst, Pregnancy outcomes. 425 432 http://ijrm.ir/browse.php?a_code=A-10-243-3&slc_lang=en&sid=1 Seyedeh Mahsa Poormoosavi m.poormoosavi@ymail.com 0000-0003-2211-1169 No Department of Histology, School of Medicine, Research and Clinical Center for Infertility, Dezful University of Medical Sciences, Dezful, Iran. Amin Behmanesh Behmanesh.ma@yahoo.com 0000-0002-4292-5381 No Department of Histology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran. Sara aryannejad saraarian@gmail.com 0000-0002-6364-583X No School of Medicine, Dezful University of Medical Sciences, Dezful, Iran. Sima Janati sjanati@ymail.com 0000-0001-5582-7177 Yes Department of Obstetrics and Gynecology, School of Medicine, Research and Clinical Center for Infertility, Dezful University of Medical Sciences, Dezful, Iran.