International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1401
7
1
gregorian
2022
10
1
20
10
online
1
fulltext
en
متابولیسم رنین-آنژیوتانسین و انکفالین در مایع فولیکولی انسان: یک مطالعه تجربی
Metabolism of renin-angiotensin and enkephalin in human follicular fluid: An experimental study
Reproductive Physiology
Original Article
Original Article
<div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">رابطه بین ویژگی­های بیوشیمیایی مایع فولیکولی (</span></span><span dir="LTR" style="font-size:10.0pt">FF</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، کیفیت تخمک و رشد جنینی هنوز مشخص نشده است. ما نمونههای </span></span><span dir="LTR" style="font-size:10.0pt">FF</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با مشخصات متابولیک طبیعی را با نمونههایی با ناهنجاریهای متابولیکی مقایسه کردیم تا نشانگرهای زیستی پیشبینیکننده بالقوه موفقیت باروری را شناسایی کنیم.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تجزیه و تحلیل فعالیت پپتیدی در </span></span><span dir="LTR" style="font-size:10.0pt">FF</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> زنان تحت لقاح آزمایشگاهی با استفاده از 3 نمونه </span></span><span dir="LTR" style="font-size:10.0pt">FF</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در هر فرد.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش­ ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نمونههای </span></span><span dir="LTR" style="font-size:10.0pt">FF</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با برداشت تخمک به دست آمد. نمونههای پاتولوژیک بهعنوان نمونههایی از </span></span><span dir="LTR" style="font-size:10.0pt">FF</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> که از زنان مبتلا به بیماریهای زنان یا ناباروری به دستآمد در نظر گرفته شد. در مجموع 30 زن در این مطالعه شرکت کردند. 3 نمونه </span></span><span dir="LTR" style="font-size:10.0pt">FF</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> از هر فرد (90 نمونه) به دست آمد، اما 8 نمونه به دلیل همولیز بودن حذف شدند. نمونه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها (</span></span><span dir="LTR" style="font-size:10.0pt">FF</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 82</span></span><span dir="LTR" style="font-size:10.0pt">n =</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) شامل افراد کنترل (36</span></span><span dir="LTR" style="font-size:10.0pt">n =</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، اهداکنندگان بدون مشکل باروری)، زنان مبتلا به آندومتریوز (15 </span></span><span dir="LTR" style="font-size:10.0pt">n =</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، ناباروری غیرقابل توضیح (19 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">n</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، و بیش از 39 سال (12 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">n</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) بودند. ما آنسفالینرژیک­های موضعی را ارزیابی کردیم: آمینوپپتیداز </span></span><span dir="LTR" style="font-size:10.0pt">N</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">(آمینوپپتیداز حساس به پورومایسین و اندوپپتیداز خنثی)؛ و اجزای سیستم آنژیوتانسین دستگاه تناسلی: پرولیل-اندوپپتیداز، </span></span><span dir="LTR" style="font-size:10.0pt">APN</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، آسپارتات-آمینوپپتیداز، و باز-آمینوپپتیداز.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هیچ تفاوتی در متابولیسم پپتید بر اساس وجود یا عدم وجود تخمک در </span></span><span dir="LTR" style="font-size:10.0pt">FF</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مشاهده نشد. زنان مبتلا به آندومتریوز و سن بالای 39 سال تغییراتی را در آمینوپپتیداز حساس به پورومایسین (01/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، آمینوپپتیداز</span></span><span lang="FA" style="font-size:10.0pt">-</span> <span dir="LTR" style="font-size:10.0pt">B</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">(01/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) آسپارتات-آمینوپپتیداز (001/0 </span></span><span lang="FA" style="font-size:10.0pt">< </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و آندوپپتیداز خنثی (001/0 </span></span><span lang="FA" style="font-size:10.0pt">< </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) نشان دادند.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه ­گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه تغییراتی را در متابولیسم انکفالین و آنژیوتانسین در </span></span><span dir="LTR" style="font-size:10.0pt">FF</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">پاتولوژیک نشان میدهد که به این مؤلفهها بهعنوان بیومارکرهای بالقوه تشخیصی اشاره میکند.</span></span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Background:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> The relationship between the biochemical characteristics of follicular fluid (FF), oocyte quality and embryonic development has not yet been elucidated. We compared samples of FF with a normal metabolic profile against samples with metabolic abnormalities to identify potential predictive biomarkers of reproductive success.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> To analyze peptide activity in the FF of women undergoing in vitro fertilization using 3 samples of FF per individual. <a name="_Hlk83295960"></a></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Materials and Methods:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> FF samples were obtained by ovum pick-up. Pathological samples were defined as samples of FF obtained from women with a gynecological condition or with infertility. A total of 30 women participated in this study. 3 samples of FF were obtained per individual (90 samples), but 8 samples were excluded because they were hemolyzed. The samples (n = 82 FF) included controls (n = 36, donors without fertility problems), women with endometriosis (n = 15), </span></span><span lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" times="">unexplained infertility</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> (n = 19), and aged > 39 (n = 12). We assessed local encephalinergics: aminopeptidase-N (puromycin sensitive aminopeptidase and neutral endopeptidase; and components of the angiotensin system of the reproductive tract: prolyl-endopeptidase, APN, aspartate-aminopeptidase, and basic-aminopeptidase. </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Results: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" times="">No differences were observed in peptide metabolism based on the presence or absence of oocytes in the FF. Women with endometriosis and aged > 39 yr showed alterations in puromycin sensitive aminopeptidase (p = 0.01), aminopeptidase-B (p = 0.01), aspartate-aminopeptidase (p < 0.001) and neutral endopeptidase (p < 0.001). </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Conclusion:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> This study reveals alterations in the metabolism of enkephalin and angiotensin in pathological FF, which points to these components as potential diagnostic biomarkers.</span></span></span></span></span></span></div>
مایع فولیکولی, پپتیدها, بیماری های تخمدان, زنان, آندومتریوز, ناباروری.
Follicular fluid, Peptides, Ovarian diseases, Women, Endometriosis, Infertility.
851
860
http://ijrm.ir/browse.php?a_code=A-10-2027-1&slc_lang=en&sid=1
Maria Victoria
Rodríguez Gallego
marivirg80@gmail.com