en بررسی مقایسه‌ای بیان ژن‌های RNF38 و P53 در نمونه‌های اسپرم به دست‌آمده از مردان مبتلا به نورمواسپرمیک و آستنوسپرمیک: یک مطالعه مورد-شاهدی Fertility & Infertility Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ناباروری به عنوان یک مشکل شایع در نظر گرفته می&shy;شود که در حدود 12-10% از زوج&shy;ها در سنین باروری ظاهر می&shy;شود. ژن </span></span><i><span dir="LTR" style="font-size:10.0pt">RNF38</span></i> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">یک پروتئین لیگاز یوبیکوئیتین است که می&shy;تواند </span></span><i><span dir="LTR" style="font-size:10.0pt">P53</span></i> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">را تنظیم کرده و بر تحرک سلولی تأثیر بگذارد. </span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">با توجه به بیان ژن </span></span><i><span dir="LTR" style="font-size:10.0pt">RNF38</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در سلول‌های نرموسپرم و آستنواسپرم و تأثیر بیان ژن </span></span><i><span dir="LTR" style="font-size:10.0pt">RNF38</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> بر آپوپتوز با القای </span></span><i><span dir="LTR" style="font-size:10.0pt">P53</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، هدف از این مطالعه بررسی بیان احتمالی این ژن و تأثیر آن بر آپوپتوز در سلول&shy;های اسپرم می&shy;باشد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش &shy;ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مطالعه حاضر بر روی 21 بیمار و 63 فرد سالم انجام شد. ابتدا از روش </span></span><span dir="LTR" style="font-size:10.0pt">Real-time PCR</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> برای اندازه&shy;گیری سطح بیان ژن&shy;های </span></span><i><span dir="LTR" style="font-size:10.0pt">P53</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:10.0pt">RNF38</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> استخراج شده از نمونه اسپرم استفاده شد و ژن </span></span><i><span dir="LTR" style="font-size:10.0pt">GAPDH</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> به عنوان ژن مرجع انتخاب شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">میزان بیان ژن‌های </span></span><i><span dir="LTR" style="font-size:10.0pt">P53</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:10.0pt">RNF38</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در نمونه‌های آستنواسپرمیک و نرمواسپرمیک به ترتیب افزایش و کاهش یافت. علاوه بر این، بین افزایش بیان ژن </span></span><span dir="LTR" style="font-size:10.0pt">P53</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و همچنین کاهش یک </span></span><i><span dir="LTR" style="font-size:10.0pt">RNF38</span></i> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و کاهش تحرک اسپرم (001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) در سلول‌های آستنواسپرم نسبت به سلول‌های نرمواسپرم تفاوت معنی‌داری مشاهده شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه&shy; گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بر اساس نتایج، افزایش بیان ژن </span></span><i><span dir="LTR" style="font-size:10.0pt">P53</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و کاهش بیان ژن </span></span><i><span dir="LTR" style="font-size:10.0pt">RNF38</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با آستنوسپرمی در مردان رابطه معنی‌داری داشت. بنابراین، انتظار می&shy;رود با استفاده از این نتایج، گامی موثر برای تشخیص الگوی بیان آستنواسپرمی اتخاذ شود.</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Background:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> Infertility is considered as a common problem appears in about 10-12% of couples in their reproductive ages. Ring finger protein 38 (RNF38) gene is a ubiquitin-protein ligase that can regulate Protein 53 (P53) and affect cellular motility. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Objective:</span></span></b> <span style="font-size:12.0pt"><span new="" roman="" times="">Considering the role of P53 on cellular motility and RNF38 on the regulation of P53, the present study aimed to assess the difference between RNF38 and P53 genes expression in normozoospermic and asthenospermic samples as a diagnostic biomarker in males.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Materials and Methods:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> The present study was conducted among 21 asthenospermics and 63 healthy individuals. First, the real-time polymerase chain reaction technique was applied to measure the expression level of the P53 and RNF38 genes extracted from sperm samples, and the glyceraldehyde-3phosphate dehydrogenase gene was selected as the reference gene.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> An increase and a decrease occurred in the level of P53 and RNF38 genes expressions in asthenospermic and normozoospermic samples, respectively. In addition, a significant difference was observed between increasing P53 gene expression (</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">p < 0.001</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">), reducing RNF38 one, and decreasing sperm motility (</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">p < 0.001</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">) in asthenospermic cells compared to that of normozoospermic ones.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Conclusion:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> Based on the results, an increase in the expression of the P53 gene and a decrease in the expression of the RNF38 gene had a significant relationship with asthenospermia in men. Therefore, it is expected that an effective step should be adopted to diagnose the asthenospermia expression pattern by using these results.</span></span></span></span></span></span></span></div> RNF38 , P53 , SDFA , Real time-PCR , Normosperm , Asthenosperm 1019 1028 http://ijrm.ir/browse.php?a_code=A-10-2065-1&slc_lang=en&sid=1 Alireza Alizadeh Alireza.email74@yahoo.com 0000-0002-1654-4462 No Department of Genetics, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran. Sina Mirzaahmadi sinacanmir@yahoo.com 0000-0003-1811-3188 Yes Department of Genetics, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran. Golnaz Asaadi Tehrani golnaz_asaadi@yahoo.com 0000-0002-3987-5766 No Department of Genetics, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran. Neda Jabbara Njabbara9@gmail.com 0000-0002-2902-4909 No Department of Genetics, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran.