en بررسی هیستوپاتولوژیک سلول های بنیادی مزانشیمی و سلول های التهابی در ضایعات آندومتریوز: یک مطالعه مورد-شاهدی Reproductive Pattology Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">آندومتریوز یک اختلال زنان و زایمان چندوجهی است که توسط یک فرآیند التهابی مزمن خوش</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">خیم وابسته به استروژن تعریف می</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">شود که در آن غدد آندومتر و بافت</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های استروما مانند در خارج از حفره رحم قرار دارند. این بیماری حدود 10-2% از تمام زنان را تحت تأثیر قرار می</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">دهد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف از این مطالعه بررسی تعداد و تنوع سلول</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های بنیادی مزانشیمی و عوامل التهابی در ضایعات آندومتریوز بود.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش &shy;ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">10 نمونه آندومتر طبیعی و آندومتر اوتوپیک به عنوان گروه شاهد و 10 نمونه خارج از رحم به عنوان گروه مورد بررسی شدند. برای ارزیابی سلول&shy;های استرومایی و سلول&shy;های التهابی از رنگ&shy;آمیزی هماتوکسیلین و ائوزین استفاده شد. رنگ&shy;آمیزی ایمونوهیستوشیمی برای نشان دادن وجود آنتی&shy;ژن هسته سلولی در حال تکثیر (</span></span><span dir="LTR" style="font-size:10.0pt">PCNA</span><span lang="FA" style="font-size:10.0pt">+</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) در ضایعات انجام شد. سلول&shy;ها هضم و در آزمایشگاه برای مطالعه تکثیر سلولی کشت داده شدند. تعداد سلول&shy;ها و عروق با &nbsp;&nbsp;نرم&shy;افزار </span></span><span dir="LTR" style="font-size:10.0pt">Image J</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">شمارش و تجزیه و تحلیل داده&shy;ها با نرم افزار </span></span><span dir="LTR" style="font-size:10.0pt">Prism</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">انجام شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تجزیه و تحلیل داده‌ها نشان داد که تعداد سلول‌های استرومایی و تعداد عروق در بافت نابجا نسبت به گروه کنترل به‌طور معنی‌داری زیاد است&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;(001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). همچنین تعداد سلول</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های التهابی شامل نوتروفیل</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها، مونوسیت</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها، لنفوسیت</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها و ماکروفاژها در گروه اکتوپیک بسیار بیشتر از گروه کنترل بود (005/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه &shy;گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">با افزایش تعداد رگ‌های خونی، جریان خون افزایش یافته و مهاجرت سلولی به بافت‌ها تسهیل می‌شود. تجمع سلول</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های التهابی، به&shy;ویژه ماکروفاژها، هم رشد سلول</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های بنیادی را تحریک می</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کند و هم با ایجاد یک فرآیند التهابی به کاشت سلول</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها کمک می</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کند.</span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:12pt"><span style="background:white"><b>Background:</b> Endometriosis is a multifaceted gynecological disorder defined as a benign estrogen-dependent chronic inflammatory process in which endometrial glands and stroma-like tissues are located outside the uterine cavity. It affects around 2-10% of all women during their reproductive years. &nbsp;</span></span><br> <span style="font-size:12pt"><span style="background:white"><b>Objective:</b> This study aimed to evaluate the traffic of mesenchymal stem cells and inflammatory factors toward the lesions.</span></span><br> <span style="font-size:12pt"><span style="background:white"><b>Materials and Methods:</b> 10 samples of normal endometrium and eutopic endometrium were studied as a control group and 10 ectopic samples were considered as a case group. Hematoxylin and eosin staining was used to evaluate stromal cells and inflammatory cells. Immunohistochemical staining was performed to show the presence of proliferating cell nuclear antigen in the lesions. The cells were digested and cultured in the laboratory to study cell proliferation. The number of cells and vessels were counted with Image J software, and data analysis was performed with Prism software.</span></span><br> <span style="font-size:12pt"><span style="background:white"><b>Results:</b> Data analysis showed that the number of stromal cells and vessels in ectopic tissue were significantly higher than the control group (p < 0.001). Also, the number of inflammatory cells, including neutrophils, monocytes, lymphocytes, and macrophages, in the ectopic group was much higher than in the control group (p < 0.005).</span></span><br> <span style="font-size:12pt"><span style="background:white"><b>Conclusion:</b> By expanding the number of blood vessels, blood flow increases, and cell migration to tissues is facilitated. The accumulation of inflammatory cells, especially macrophages, stimulates the growth of stem cells and helps implant cells by creating an inflammatory process.</span></span></span></div> آندومتریوز, PCNA, سلول‌های بنیادی, التهاب. Endometriosis, PCNA, Stem cell, Inflammation. 819 830 http://ijrm.ir/browse.php?a_code=A-10-2168-1&slc_lang=en&sid=1 Ali Mohebbi alimohebbi.tums@gmail.com 0000-0001-6774-3878 No Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran. Vida Hojati V.Hojati@damghaniau.ac.ir 0000-0002-0408-389X Yes Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran. Masoumeh Majidi Zolbin Masoumeh.majidizolbin@gmail.com 0000-0002-1351-8955 No Pediatric Urology and Regenerative Medicine Research Center, Gene, Cell, and Tissue Research Institute, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. Reza Aflatoonian r.aflatoonian@gmail.com 0000-0002-5914-8662 No Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.