en Stem Cell & Cloning Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b>Background: </b>Fibroblasts from different parts of the human body have been used in cell biology, drug discovery and cell therapy studies. One of the most available sources of human fibroblasts is neonatal foreskin. Not only do these cells have wound-healing applications, but they are also the most popular source for pluripotent stem cell biotechnology. Moreover, several studies have indicated that different sources of fibroblasts display similar features to mesenchymal stem cells.</span></span></span><br> <span style="font-size:12pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b>Objective:</b> Generation and establishment of new human foreskin fibroblast cell lines called Yazd human foreskin fibroblasts (YhFFs).</span></span></span><br> <span style="font-size:12pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b>Materials and Methods:</b> In this lab resources study, the production of 3 YhFF cell lines (YhFF#8, YhFF#17, and YhFF#18) is reported. Their biological features were characterized using immunofluorescence, polymerase chain reaction, and flow-cytometry for mesenchymal markers such as fibronectin, vimentin, CD44, CD73, CD90, CD105, and hematopoietic markers CD34 and CD45.</span></span></span><br> <span style="font-size:12pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b>Results:</b> The YhFF cell lines were passaged more than 40 times and their normal karyotype was checked using G-binding. Similarly to previous reports, the flow cytometry analysis revealed that the YhFF cell lines displayed mesenchymal stromal cell characteristics.</span></span></span><br> <span style="font-size:12pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b>Conclusion:</b> This study will contribute to the development of clinical-grade cell-based products such as micro-vesicles and exosomes for future therapeutic applications in regenerative medicine.&nbsp;</span></span></span></div> Cell therapy, Fibroblasts, Mesenchymal stem/stromal cells, Human embryonic stem cells, Micro-vesicles. 519 528 http://ijrm.ir/browse.php?a_code=A-10-588-4&slc_lang=en&sid=1 Fatemeh Hajizadeh-Tafti fhajizade69@yahoo.com 0000-0003-3067-0534 No Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Jalal Golzadeh golzadehjalal@gmail.com 0000-0002-3425-9526 No Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Ehsan Farashahi-Yazd ehsanfarashahi@gmail.com 0000-0003-1122-0626 No Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Hassan Heidarian-Meimandi heidarianmh@gmail.com 0000-0001-6283-9053 No Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Behrouz Aflatoonian b.aflatoonian@ssu.ac.ir 0000-0002-7250-9813 Yes Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Medical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Advanced Medical Sciences and Technologies in Medical Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.