International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1401
9
1
gregorian
2022
12
1
20
12
online
1
fulltext
en
سراتونیا سیلیکا آسیبهای ناشی از انجماد را در نمونههای آستنوزواسپرمیا بهبود می بخشد: یک مطالعه تجربی
Ceratonia siliqua L improved cryodamage of asthenozoospermic specimens: An experimental study
Fertility & Infertility
Original Article
Original Article
<div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">انجماد اسپرم یک روش مهم در تکنولوژی کمک باروری می</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">باشد. انجماد منجر به تغییرات شیمیایی و فیزیکی در اسپرم می</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">شود. سراتونیا سیلیکا درختی است که دارای خواص آنتی</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">اکسیدانی می</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">باشد.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه با هدف بررسی اثر غلظت</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های مختلف عصاره سراتونیا سیلیکا در محیط فریز بر روی پارامترهای سیمن، و برخی پارامترهای بیوشیمیایی در نمونه</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های آستنوزواسپرمیا انجام شد.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش­ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">40 نمونه آستنوزواسپرمیا (نمونه­های سیمن با تحرک کمتر از 32%) از مردان 40-20 ساله بر اساس معیارهای سازمان بهداشت جهانی در این مطالعه بدست آمد. هر نمونه به 6 گروه تقسیم شد: گروه </span></span><span dir="LTR" style="font-size:10.0pt">fresh</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">(گروه 1)، در گروه</span></span><span dir="LTR" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های 2 تا 6، به ترتیب 0 (به عنوان گروه کنترل)، 5، 10، 20 و 30 میکروگرم بر میلی</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">لیتر عصاره سراتونیا سیلیکا به محیط انجماد به ترتیب اضافه شد. سپس پارامترهای اسپرم، مالون­دی­آلدئید، ظرفیت تام آنتی­اکسیدانی، گونه­های فعال اکسیژن و ارزیابی </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">اسپرم با استفاده از پروتکل</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های مرتبط پس از ذوب مورد ارزیابی قرار گرفت.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تجزیه و تحلیل داده</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها نشان داد که پارامترهای اسپرم و سطح تام آنتی­اکسیدانی در غلظت 20 میکروگرم بر میلی</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">لیتر عصاره سراتونیا سیلیکا در مقایسه با سایر غلظت</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های عصاره سراتونیا سیلیکا بعد از انجماد و ذوب افزایش یافت (001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). هم</span></span><span dir="LTR" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">چنین، سطوح گونه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های فعال اکسیژن، ارزیابی </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">اسپرم و مالون</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">دی</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">آلدئید نیز با افزودن غلظت 20 میکروگرم بر میلی</span></span><span dir="LTR" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">لیتر عصاره سراتونیا سیلیکا به محیط انجماد اسپرم نسبت به سایر گروه</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های تیمار شده پس از انجماد به</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">طور معناداری کاهش یافت (001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). </span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه­گیری:</span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> عصاره سراتونیا سیلیکا پارامترهای اسپرم را پس از انجماد و ذوب در نمونه­های آستنوزواسپرمیا به طور معناداری بهبود بخشید، و بیشترین تأثیر در غلظت 20 میکروگرم بر میلی­لیتر عصاره سراتونیا سیلیکا مشاهده شد (001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></span></span></span></span></span><br>
</div>
<div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Abstract</span></span></b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Background:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> Sperm freezing is an important procedure in assisted reproductive technology. Freezing results in physical and chemical changes in the sperm. <i>Ceratonia siliqua L</i> (<i>C.siliqua</i>) is a tree that has antioxidant properties.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> This study aimed to investigate the effect of different concentrations of <i>C.siliqua </i>in a freezing medium on semen parameters, and some biochemical parameters in asthenozoospermic specimens.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Materials and Methods:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> 40 asthenozoospermic specimens </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">(semen specimens with motility < 32%) </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">were obtained from </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">men aged between 20-40 yr</span></span> <span style="font-size:12.0pt"><span new="" roman="" times="">according to the World Health Organization criteria</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">. Each sample was divided into 6 groups: I) fresh, II) control, III) 5, IV) 10, V) 20, and VI) 30 µg/ml <i>C.siliqua</i> extract were added to a freezing medium respectively. Then sperm parameters, </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">malondialdehyde, total antioxidant capacity</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">, </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">reactive oxygen species</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">, and sperm DNA assay were evaluated using related protocols after thawing.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> Data analysis shows that sperm parameter, and total antioxidant capacity level increased at a concentration of 20 µg/ml of <i>C. siliqua</i> extract compared to the other concentrations of <i>C.siliqua</i> extract after cryopreservation and thawing (p < 0.001). Also, the </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">sperm DNA fragmentation assay</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">, reactive oxygen species, and malondialdehyde levels were significantly reduced by adding 20 µg/ml of <i>C. siliqua</i> extract to the sperm freezing medium compared to the other treated groups after cryopreservation (p < 0.001). </span></span></span></span></span><br>
<b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" times="">Conclusion:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" times=""> <i>C.siliqua</i> extract significantly improved sperm parameters after cryopreservation and thawing in asthenozoospermic specimens,</span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" times=""> and the greatest impact was observed at the 20 </span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" times="">µg/ml </span></span></span><i><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:115%"><span new="" roman="" times="">C.siliqua L</span></span></span></span></i><span lang="EN" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" times=""> extract concentration (p < 0.001)</span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" times="">.</span></span></span></span></div>
Asthenozoospermia, Ceratonia, Cryopreservation, Fertility preservation, Infertility, Male.Infertility.
1029
1038
http://ijrm.ir/browse.php?a_code=A-10-2234-1&slc_lang=en&sid=1
Fatemeh
Javanmard
fa.javanmard@yahoo.com