en تستوسترون پروپیونات و Swarna Bhasma تغییرات تولید مثلی ناشی از D-گالاکتوز را در موش های صحرایی نر ویستار تعدیل کردند: یک مطالعه تجربی Reproductive Biology Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">سیستم تناسلی مردان تغییرات نامطلوب متعددی را در ارتباط با سن مانند کاهش سنتز هورمون، تعداد اسپرم و تغییرات بیضه نشان می&shy;دهد که می&shy;تواند بر باروری تأثیر بگذارد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه با هدف بررسی اثرات تستوسترون پروپیونات (</span></span><span dir="LTR" style="font-size:10.0pt">TP</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و نوع آیورودیک</span></span><i><span dir="LTR" style="font-size:10.0pt">Swarna Bhasma </span></i><i>&nbsp;</i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">(</span></span><span dir="LTR" style="font-size:10.0pt">SB</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) بر پیری باروری ناشی از </span></span><span dir="LTR" style="font-size:10.0pt">D-galactose</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (</span></span><span dir="LTR" style="font-size:10.0pt">D-gal</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) در موش‌های صحرایی نر نژاد ویستار انجام شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش&shy; ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">60 سر موش صحرایی نر نژاد ویستار به 10 گروه 6 تایی تقسیم شدند. پیری باروری با قرار گرفتن در معرض </span></span><span dir="LTR" style="font-size:10.0pt">D-gal</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (</span></span><span dir="LTR" style="font-size:10.0pt">mg/kg Bwt</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 150) به مدت 60 روز القا شد. سپس موش‌ها تحت درمان تنها و ترکیبی با </span></span><span dir="LTR" style="font-size:10.0pt">TP</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (</span></span><span dir="LTR" style="font-size:10.0pt">mg/kg Bwt</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 2) و</span></span><span dir="LTR" style="font-size:10.0pt">SB </span>&nbsp;<span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">(</span></span><span dir="LTR" style="font-size:10.0pt">mg/kg Bwt</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 75/6) قرار گرفتند. سپس پارامترهای اسپرم، هورمون&shy;های تولیدمثلی، نشانگرهای التهابی، آنزیم&shy;های آنتی&shy;اکسیدانی بیضه، آنزیم&shy;های استروئیدزا و تظاهرات بافتی بیضه مورد بررسی قرار گرفت.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواجهه با </span></span><span dir="LTR" style="font-size:10.0pt">D-gal</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">باعث کاهش معنی‌دار (001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) تستوسترون سرم، آنزیم‌های استروئیدوژن بیضه و آنتی‌اکسیدان شد. تجویز </span></span><span dir="LTR" style="font-size:10.0pt">TP</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> سطح </span></span><span dir="LTR" style="font-size:10.0pt">T</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> سرم، آنزیم</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های آنتی</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">اکسیدانی بیضه و پروفایل اسپرم</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">زایی را در سطح معنی</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">داری (001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) در مقایسه با </span></span><span dir="LTR" style="font-size:10.0pt">D-gal</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> افزایش داد. علاوه بر این، تیمار </span></span><span dir="LTR" style="font-size:10.0pt">SB</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> به طور قابل توجهی (001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) سطح </span></span><span dir="LTR" style="font-size:10.0pt">T</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> سرم، تعداد اسپرم، آنزیم&shy;های آنتی&shy;اکسیدانی بیضه و آنزیم&shy;های استروئیدوژن را در مقایسه با </span></span><span dir="LTR" style="font-size:10.0pt">D-gal</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> افزایش داد. </span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه&shy; گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هر دو درمان </span></span><span dir="LTR" style="font-size:10.0pt">TP</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">SB</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> اختلالات تولیدمثلی ناشی از </span></span><span dir="LTR" style="font-size:10.0pt">D-gal</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> را بهبود دادند. با این حال، مکمل </span></span><span dir="LTR" style="font-size:10.0pt">T</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> اگزوژن از طریق تجویز </span></span><span dir="LTR" style="font-size:10.0pt">TP</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با عوارض جانبی مختلفی در طول استفاده طولانی مدت همراه است. </span></span><span dir="LTR" style="font-size:10.0pt">SB</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> یک فرمول آیورودیک است که سابقه طولانی استفاده در هند دارد. یافته</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های فعلی نشان می</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">دهد که </span></span><span dir="LTR" style="font-size:10.0pt">SB</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> ممکن است به عنوان یک جایگزین خوب برای تقویت عملکرد تولید&shy;مثل در مردان مسن استفاده شود.</span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background:</span></span></b><span style="font-size:12.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""> The male reproductive system undergoes several adverse age-related changes</span></span></span><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> like decreased hormone synthesis, sperm count, and testicular alteration that can impact on fertility. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objective:</span></span></b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The study aims to investigate the effects of testosterone propionate (TP), and ayurvedic formulation <i>Swarna Bhasma</i> (SB) on D-galactose (D-gal) induced reproductive aging in male Wistar rats.<b> </b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> 60 male Wistar rats were divided into 10 groups of 6 animals. Reproductive aging was induced by D-gal (150 mg/kg Bwt) exposure for 60 days. The rats were then treated by post and combination treatment with TP (2 mg/kg Bwt) and SB (6.75 mg/kg Bwt). Then sperm parameters, reproductive hormones, inflammatory markers, testicular antioxidant enzymes, steroidogenic enzymes, and histological manifestation of testis were evaluated. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Exposure of D-gal caused significant (p < 0.001) decrease in serum testosterone (T), testicular steroidogenic, and antioxidant enzymes. Administration of TP increased the serum T level, testicular antioxidant enzymes, and spermatogenic profile at a significant level of (p < 0.001) compared to D-gal. Further, the SB treatment significantly (p < 0.001) elevated the serum T level, sperm count, testicular antioxidant enzymes, steroidogenic enzymes, when compared to D-gal. </span></span><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Both the treatment of TP and SB treatments recovered the reproductive impairments caused by D-gal. However, exogenous T supplementation via TP administration is associated with various side effects during long-term use. SB is an Ayurvedic formulation having a long history of usage in India. The current findings suggest that the SB may be used as a good alternative for potentiating reproductive function in aging males. </span></span></span></span></span></div> <br> <quillbot-extension-portal></quillbot-extension-portal><quillbot-extension-portal></quillbot-extension-portal><quillbot-extension-portal></quillbot-extension-portal><quillbot-extension-portal></quillbot-extension-portal><quillbot-extension-portal></quillbot-extension-portal> دی گالاکتوز, پیری, تستوسترون پروپیونات, Swarna Bhasma, بیضه, هیپوگنادیسم, ناباروری. D-galactose, Aging, Testosterone propionate, Swarna Bhasma, Testis, Hypogonadism, infertility. 303 322 http://ijrm.ir/browse.php?a_code=A-10-2277-2&slc_lang=en&sid=1 Aashish Kumar Netam aashishnetam@gmail.com 0000-0001-5809-9451 No Department of Zoology, Indira Gandhi National Tribal University, Amarkantak, Madhya Pradesh, India. Vikas Pankaj Bhargava vikas.luvs@gmai.com 0000-0001-7721-6609 No Department of Zoology, Indira Gandhi National Tribal University, Amarkantak, Madhya Pradesh, India. Rambir Singh sehrawat_r@yahoo.com 0000-0001-5654-8030 No Department of Horticulture, Aromatic and Medicinal Plants, Mizoram University, Aizawl, Mizoram, India. Poonam Sharma pnm245@yahoo.com 0000-0003-1999-7309 Yes Department of Zoology, Indira Gandhi National Tribal University, Amarkantak, Madhya Pradesh, India.