en مقایسه رشد فولیکولی تخمدان موش و بیان ژن در حضور سلنیت سدیم و عصاره بافت تخمدان: یک مطالعه تجربی Reproductive Biology Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">سدیم سلنیت (</span></span><span dir="LTR" style="font-size:10.0pt">SS</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و عصاره بافت تخمدان (</span></span><span dir="LTR" style="font-size:10.0pt">OTE</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) رشد و بلوغ فولیکول&shy;های پره&shy;آنترال را به صورت وابسته به دوز افزایش می&shy;دهند.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">اثرات </span></span><span dir="LTR" style="font-size:10.0pt">SS</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">OTE</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> را بر بیان ژن&shy;های رسپتور هورمون محرک فولیکولی (</span></span><span dir="LTR" style="font-size:10.0pt">FSHR</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و آنتی&shy;ژن هسته&shy;ای تکثیر سلولی (</span></span><span dir="LTR" style="font-size:10.0pt">PCNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) در فولیکول&shy;های بلوغ یافته بررسی شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش&shy; ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">عصاره بافت تخمدان از موش&shy;های بالغ تهیه شد. فولیکول‌های پره&shy;آنترال (266 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">n</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) از موش&shy;های 16-12 روزه جداشده و در سه گروه کنترل،</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تجربی</span></span> <span dir="LTR" style="font-size:10.0pt">I</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">(10 نانوگرم بر میلی</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">لیتر</span></span><span dir="LTR" style="font-size:10.0pt">SS </span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و تجربی</span></span> <span dir="LTR" style="font-size:10.0pt">II</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">(</span></span><span dir="LTR" style="font-size:10.0pt">OTE</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به مدت 12 روز کشت شدند. قطر فولیکولی، بقا و میزان بلوغ فولیکولی، تولید 17</span></span><span lang="FA" style="font-size:10.0pt">-</span><span dir="LTR" style="font-size:10.0pt">&beta;</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> استرادیول و پروژسترون و بیان ژن&shy;های رسپتور هورمون محرک فولیکولی و آنتی&shy;ژن هسته&shy;ای تکثیر سلولی مورد بررسی قرار گرفت. </span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">میزان بقا فولیکول</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها در گروه تحت درمان </span></span><span dir="LTR" style="font-size:10.0pt">SS</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">(58/84%) در مقایسه با گروه کشت شده با </span></span><span dir="LTR" style="font-size:10.0pt">OTE</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (023/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، 63/75%) و گروه کنترل (032/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، 38/69%) افزایش معنی</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">داری داشت. میانگین قطر فولیکول‌ها (</span></span><span dir="LTR" style="font-size:10.0pt">&micro;m</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) در گروه تحت درمان با </span></span><span dir="LTR" style="font-size:10.0pt">SS</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (8/403) و </span></span><span dir="LTR" style="font-size:10.0pt">OTE</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">(97/383) به‌طور معنی‌داری بیشتر از کنترل (05/342) (032/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) بود. تکوین فولیکولی، شکل</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">گیری انتروم، و آزادسازی تخمک متافاز </span></span><span dir="LTR" style="font-size:10.0pt">II</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (به ترتیب 019/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، 027/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) تولید هورمون</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های 17 بتا استرادیول و پروژسترون، و بیان ژن</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های مورد مطالعه در هر دو گروه آزمایشی به طور معنی</span></span><span lang="FA" style="font-size:11.0pt"><span cambria="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">داری بیشتر از گروه کنترل بود (به ترتیب 021/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، 023/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه &shy;گیری: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">اثرات مثبت</span></span> <span dir="LTR" style="font-size:10.0pt">OTE</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">SS</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> بر رشد و بلوغ فولیکول‌های پره&shy;آنترال موش از مسیر افزایش بیان ژن&shy;های</span></span> <i><span dir="LTR" style="font-size:10.0pt">FSHR</span></i> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و</span></span> <i><span dir="LTR" style="font-size:10.0pt">PCNA</span></i> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">می&shy;باشد.</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Ovarian tissue extract (OTE) and sodium selenite (SS) enhance the growth and maturation of preantral follicles in a dose-dependent manner. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The present study was designed to bring more information regarding the mechanism of OTE and SS on the mRNA expression of <span style="background:white">follicle-stimulating hormone receptors (FSHR) and the proliferation cell nuclear antigens (PCNA) of in vitro matured isolated follicles.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The tissue extract was prepared from adult ovaries. The preantral follicles (n = 266) were isolated from 12-16-day-old mice and cultured in the control, experimental I (10 ng/ml SS), and experimental II (OTE) groups for 12 days. The follicular diameter, survival, and maturation rates, also, the production of 17-&beta;-estradiol and progesterone, and the follicular expression of <i><span style="background:white">PCNA</span></i> and <i><span style="background:white">FSH </span></i><span style="background:white">receptor</span> genes were analyzed. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The survival rate of follicles in the SS-treated group (84.58%) was significantly higher than that OTE (75.63%; p = 0.023) and control (69.38%; p = 0.032) groups. The mean diameter of culture follicles in experimental group I (403.8 &mu;m) and experimental group II (383.97 &mu;m) increased significantly in comparison with the control group (342.05 &mu;m; p = 0.032). The developmental rate of follicles, percentages of antrum formation, released metaphase II oocytes (p = 0.027; p = 0.019 respectively), production of hormones and the expression of 2 studied genes were significantly increased in both experimental groups in compare with control group (p = 0.021; p = 0.023 respectively).</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The OTE and SS have a positive effect on development of mouse preantral follicles via over-expression of <i>FSHR</i> and <i>PCNA</i> genes. </span></span></span></span></span></span></div> رسپتور هورمون محرک فولیکولی, تخمدان, سلنیت سدیم, آنتی­ژن هسته­ای تکثیر سلولی, موش. Follicle-stimulating hormone receptor, Ovary, Sodium selenite, Proliferation cell nuclear antigen, Mouse. 415 424 http://ijrm.ir/browse.php?a_code=A-10-158-4&slc_lang=en&sid=1 Hamid Shoorei h.shoorei@gmail.com 0000-0002-7762-5362 No Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Department of Anatomical Sciences, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran. Mina Jafarabadi minajaf@yahoo.com 0000-0002-7762-5362 No Vali-E-Asr Reproductive Health Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Shahram PourBayranvand shahrampb@modares.ac.ir 0000-0002-7762-5362 No Vali-E-Asr Reproductive Health Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Mojdeh Salehnia salehnim@modares.ac.ir 0000-0002-7762-5362 Yes Vali-E-Asr Reproductive Health Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran.