en اثر منفی واریکوسل بر اختلال عملکرد میتوکندری اسپرم: مطالعه مقطعی Reproductive Urology Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">واریکوسل اتساع و بزرگ شدن غیرطبیعی شبکه پامپینیفورم وریدی کیسه بیضه است که در تخلیه طبیعی خون اختلال ایجاد می&shy;کند و در نهایت در صورت عدم درمان منجر به ناباروری می&shy;شود.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف ما کشف تأثیر وضعیت میتوکندری از طریق ارزیابی </span></span><span dir="LTR" style="font-size:10.0pt">MMP</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و </span></span><span dir="LTR" style="font-size:10.0pt">ATP</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و ارتباط آن با پارامترهای مایع منی برای نشان دادن تأثیر سلامت میتوکندری اسپرم بر عملکرد طبیعی اسپرم بود.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش &shy;ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه مقطعی تحلیلی بر روی 100 مرد شامل 50 مورد در گروه نورموزواسپرمی (نرمال) و 50 نفر در گروه نابارور که تحت عمل جراحی واریکوسلکتومی (واریکوسل) قرار نگرفته بودند و به مرکز تحقیقات و درمان ناباروری جهاد دانشگاهی خوزستان، مراجعه داشته صورت پذیرفت. تجزیه و تحلیل معمول مایع منی بر اساس دستورالعمل&shy;های سازمان بهداشت جهانی، شاخص قطعه&shy;قطعه شدن </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، سنجش </span></span><span dir="LTR" style="font-size:10.0pt">MMP</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، محتوای </span></span><span dir="LTR" style="font-size:10.0pt">ATP</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و آپوپتوز برای همه نمونه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها طبق دستورالعمل انجام شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج این مطالعه نشان داد که غلظت، درصد تحرک پیشرونده، مورفولوژی طبیعی، درصد </span></span><span dir="LTR" style="font-size:10.0pt">MMP</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">ATP</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> اسپرم در واریکوسل به‌طور معنی‌داری کمتر از گروه نرمال بود. علاوه بر این، درصد </span></span><span dir="LTR" style="font-size:10.0pt">DFI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> اسپرم در بیماران واریکوسل در مقایسه با گروه نرمال به طور معنی‌داری بیشتر بود.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه &shy;گیری:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کاهش محتوای </span></span><span dir="LTR" style="font-size:10.0pt">MMP</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">ATP</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، علاوه بر کاهش کیفیت پارامترهای اسپرم و افزایش تکه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تکه شدن </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> اسپرم، تأثیر جدی بر اختلال عملکرد میتوکندری اسپرم در بیماران واریکوسل دارد.</span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Background: </b>Varicocele is an abnormal dilation and enlargement of the scrotal venous pampiniform plexus that impairs normal blood drainage and finally leads to infertility if not treated.</span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><strong>Objective: </strong>This study aimed to figure out the impact of mitochondria status through the mitochondrial membrane potential (MMP) and adenosine triphosphate (ATP) assessment and its correlation with semen parameters to illuminate the impact of sperm mitochondria healthiness on normal sperm functionality.</span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><strong>Materials and Methods:</strong> This analytical cross-sectional study was conducted with 100 men including 50 cases in the normozoospermic group (normal) and 50 in an infertile group with the non-varicocelectomy operation (varicocele) referring to Infertility Research and Treatment Center, ACECR Khuzestan, Iran. Routine semen analysis was performed according to World Health Organization guidelines, DNA fragmentation index, the MMP assay, ATP content, and apoptosis were carried out for all samples.</span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><strong>Results:</strong> The results showed that the concentration, progressive motility, normal morphology, MMP, and ATP contents of sperm in varicocele were significantly lower than the normal group. In addition, the sperm DNA fragmentation index was significantly higher in the varicocele group in comparison with the normal group.</span></span><br> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><strong>Conclusion:</strong> Reduction in MMP and ATP contents, besides the loss of sperm parameters quality and increase in sperm DNA fragmentation, were seriously implicating sperm mitochondria dysfunctionality in varicocele men.</span></span></div> میتوکندری, ATP, آپوپتوز, واریکوسل, ناباروری مردان. Mitochondria, ATP, Apoptosis, Varicocele, Male infertility. 323 332 http://ijrm.ir/browse.php?a_code=A-10-2340-1&slc_lang=en&sid=1 Mahshid Elahi mahshidelahy@gmail.com 0000-0002-6023-8075 No Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran. Vida Hojati V.hojati@damghaniau.ac.ir 0000-0002-0408-389X No Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran. Mahmoud Hashemitabar hashemi-m@ajums.ac.ir 0000-0002-8800-3761 No Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Mahsa Afrough m.afrough@gmail.com 0000-0003-4744-236X No Health Education Research Department, ACECR, Khuzestan, Ahvaz, Iran. Hossain Mohammadpour Kargar pourkargar@yahoo.com 0000-0001-9697-8369 No Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran. Maryam Dastoorpoor mdastoorpour@yahoo.com 0000-0001-5268-334X Yes Department of Biostatistics and Epidemiology, Social Determinants of Health Research Center, Ahvaz Jondishapur University of Medical Sciences, Ahvaz, Iran.