International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1403
1
1
gregorian
2024
4
1
22
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online
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fulltext
en
تأثیر انجماد شیشه ای اسپرم در محیط بدون عوامل محافظ انجماد در مویرگهای شیشه ای کشیده بر پارامترهای اسپرم و یکپارچگی DNA: یک مطالعه آزمایشی آزمایشگاهی
Impact of cryoprotectant-free sperm vitrification in pulled-glass capillary on sperm parameters and DNA integrity: A lab trial study
Reproductive Andrology
Original Article
Original Article
<div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">انجماد شیشه­ای یک تکنیک کمک باروری است که اخیراً معرفی شده و در عین حال کاربرد گسترده­ای دارد. در حال حاضر، اثرات مواد شیمیایی و وسایل انجماد بر تحرک اسپرم و یکپارچگی </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">هنوز نامشخص است.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">این مطالعه با هدف بررسی کیفیت اسپرم به وسیله تجزیه و تحلیل مایع منی و یکپارچگی </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">اسپرم، در هنگام انجماد با یا بدون عوامل مجافظ انجماد (</span></span><span dir="LTR" style="font-size:10.0pt">CPAs</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) با استفاده از مویرگهای شیشه</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif"">­</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">ای کشیده انجام گرفت.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">مواد و روش ­ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">بین فوریه و ژوئن 2020، 50 مرد نابارور مراجعه­کننده به مرکز غدد و باروری و ناباروری </span></span><span dir="LTR" style="font-size:10.0pt">Hue</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">، دانشگاه پزشکی و داروسازی </span></span><span dir="LTR" style="font-size:10.0pt">Hue</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">، ویتنام، وارد مطالعه شدند. نمونههای اسپرم تهیه شده با استفاده از تکنیک شنا کردن به 2 گروه انجماد شیشهای با </span></span><span dir="LTR" style="font-size:10.0pt">CPA</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> (گروه 1) و بدون </span></span><span dir="LTR" style="font-size:10.0pt">CPA</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">(گروه 2) تقسیم شدند. نمونههای اسپرم منجمد شده در مویرگهای شیشهای 10 میکرولیتری نگهداری شدند. تحرک، یکپارچگی غشای اسپرم و شاخص تکه­تکه شدن </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> مورد آزمایش قرار گرفتند.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">تحرک اسپرم در محیط­های منجمد شده با </span></span><span dir="LTR" style="font-size:10.0pt"> CPA</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">(11% </span></span><span dir="LTR" style="font-size:10.0pt">±</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> 4/54) از نظر آماری بیشتر از محیط­های بدون </span></span><span dir="LTR" style="font-size:10.0pt">CPA</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> بود (05/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt">،</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> 6%/10 </span></span><span dir="LTR" style="font-size:10.0pt">±</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> 14/51) بر یکپارچگی غشای اسپرم یا </span></span><span dir="LTR" style="font-size:10.0pt">large halo ratio</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> تأثیری نداشت (به ترتیب 47/8 </span></span><span dir="LTR" style="font-size:10.0pt">±</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> 34/71 در مقابل 11/8 </span></span><span dir="LTR" style="font-size:10.0pt">±</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> 38/70 و 92/18 </span></span><span dir="LTR" style="font-size:10.0pt">±</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> 84/50 در مقابل 44/19 </span></span><span dir="LTR" style="font-size:10.0pt">±</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> 98/51). گروه 2 شاخص قطعه ­قطعه شدن </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> کمتری نسبت به گروه 1 پس از انجماد شیشه­ای نشان دادند (47/8 </span></span><span lang="FA" style="font-size:10.0pt">±</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> 2/14 در مقابل 03/9 </span></span><span lang="FA" style="font-size:10.0pt">±</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> 60/12، 021/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">).</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتیجه­ گیری: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">در هنگام استفاده از مویرگ شیشهای کشیده برای انجماد اسپرم، وجود </span></span><span dir="LTR" style="font-size:10.0pt">CPAs</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> در محیط انجماد منجر به تحرک پیشرونده بالاتر و شاخص تکه</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif"">­</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">تکه شدن </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> کمتری نسبت به محیط بدون </span></span><span dir="LTR" style="font-size:10.0pt">CPA</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> می­شود.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:13pt"><span style="line-height:normal"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:12.0pt">Background:</span></b><span lang="VI" style="font-size:12.0pt"> Vitrification is a recently introduced </span><span style="font-size:12.0pt">yet</span><span lang="VI" style="font-size:12.0pt"> widely applied assisted reproduction technique. </span><span style="font-size:12.0pt">So far</span><span lang="VI" style="font-size:12.0pt">, the effects of </span><span style="font-size:12.0pt">t</span><span lang="VI" style="font-size:12.0pt">he chemicals and </span><span style="font-size:12.0pt">device</span><span lang="VI" style="font-size:12.0pt">s </span><span style="font-size:12.0pt">in </span><span lang="VI" style="font-size:12.0pt">vitrification on sperm motility and DNA integrity are still unclear</span><span style="font-size:12.0pt">.</span><b><span style="font-size:12.0pt"></span></b></span></span></span><br>
<span style="font-size:13pt"><span style="line-height:normal"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:12.0pt">Objective</span></b><b><span lang="VI" style="font-size:12.0pt">:</span></b><span lang="VI" style="font-size:12.0pt"> T</span><span style="font-size:12.0pt">his study</span><span lang="VI" style="font-size:12.0pt"> aimed</span><span style="font-size:12.0pt"> to examine sperm quality, as determined by semen analysis and sperm DNA integrity when vitrified with or without cryoprotectant agents </span><span lang="VI" style="font-size:12.0pt">(CPA</span><span style="font-size:12.0pt">s</span><span lang="VI" style="font-size:12.0pt">) </span><span style="font-size:12.0pt">using pulled-glass capillaries.</span></span></span></span><br>
<span style="font-size:13pt"><span style="line-height:normal"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:12.0pt">Materials and Methods</span></b><b><span lang="VI" style="font-size:12.0pt">:</span></b><span lang="VI" style="font-size:12.0pt"> Between February and June 2020, 50 infertile men from the Hue Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Vietnam, were enrolled</span><span style="font-size:12.0pt">. Sperm samples, prepared using the swim-up technique, were divided into 2 groups:</span><span lang="VI" style="font-size:12.0pt"> vitrification with CPAs (group 1) and without CPAs (group 2). </span><span style="font-size:12.0pt">Vitrified sperm samples were preserved in 10 µL pulled-glass capillaries. Motility</span><span lang="VI" style="font-size:12.0pt">, </span><span style="font-size:12.0pt">sperm membrane integrity, and the DNA fragmentation index were tested.</span></span></span></span><br>
<span style="font-size:13pt"><span style="line-height:normal"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:12.0pt">Results</span></b><b><span lang="VI" style="font-size:12.0pt">:</span></b> <span lang="VI" style="font-size:12.0pt">Sperm motility in vitrified media with CPAs (54.4 ± 11%) was statistically higher than in media without CPAs (51.14 ± 10.6%, p < 0.05). CPAs did not affect sperm membrane integrity or large halo ratio (71.34 ± 8.47 vs. 70.38 ± 8.11 and 50.84 ± 18.92 vs. 51.98 ± 19.44, respectively). Group 2 exhibited a lower DNA fragmentation index than group 1 after vitrification (14.2 ± 8.47 vs. 12.60 ± 9.03, p = 0.021).</span><span style="font-size:12.0pt"></span></span></span></span><br>
<span style="font-size:13pt"><span style="line-height:normal"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:12.0pt">Conclusion:</span></b><span style="font-size:12.0pt"> Using a pulled-glass capillary for sperm vitrification, the presence of CPAs in the vitrification </span><span lang="VI" style="font-size:12.0pt">medium</span><span style="font-size:12.0pt"> resulted in higher progressive motility </span><span lang="VI" style="font-size:12.0pt">and</span><span style="font-size:12.0pt"> lower DNA fragmentation index than the </span><span lang="VI" style="font-size:12.0pt">medium</span><span style="font-size:12.0pt"> without CPAs. </span></span></span></span></div>
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<quillbot-extension-portal></quillbot-extension-portal><quillbot-extension-portal></quillbot-extension-portal>
انجماد, اسپرم, قطعهقطعه شدن DNA, عوامل سرمایشی, حجم کم.
Vitrification, Sperm, DNA fragmentation, Cryoprotectant agents, Small volume.
305
316
http://ijrm.ir/browse.php?a_code=A-10-1567-2&slc_lang=en&sid=1
Minh Tam
Le
leminhtam@huemed-univ.edu.vn