en تعیین زمان بیان کمپلکس اینفلامازوم Nod-like receptor family Pyrin domain containing 3 در بافت بیضه موش های صحرایی مبتلا به واریکوسل: یک مطالعه آزمایشگاهی Reproductive Anatomy Original Article Original Article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span b="" lang="FA" mitra="" style="font-family:">مقدمه:</span></b><b> </b><span b="" lang="FA" mitra="" style="font-family:">واریکوسل با گشادشدگی غیرعادی در ورید تستیکولار مشخص شده و باعث هایپوکسی، تجمع رادیکال&shy;های آزاد، افزایش تولید سیتوکین&shy;های پیش&shy;التهابی می&shy;شود. به نظر می&shy;رسد گروهی از گیرنده&shy;های سیتوپلاسمی به نام اینفلامازوم (</span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NLRP3</span></span><span b="" lang="FA" mitra="" style="font-family:">)</span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">nod-like receptor family</span></span><span arial="" dir="LTR" style="font-family:">, </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">pyrin domain</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">containing3 </span></span><span b="" lang="FA" mitra="" style="font-family:">در طی واریکوسل فعال شده و در پاتوژنز آن دخیل است.</span><span dir="LTR" style="font-family:"Arial","sans-serif""></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span b="" lang="FA" mitra="" style="font-family:">هدف: </span></b><span b="" lang="FA" mitra="" style="font-family:">هدف ما تعیین زمان بیان اینفلامازوم </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NLRP3</span></span> <span b="" lang="FA" mitra="" style="font-family:">در بافت بیضه به دنبال القای واریکوسل است.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span b="" lang="FA" mitra="" style="font-family:">مواد و روش&shy; ها: </span></b><span b="" lang="FA" mitra="" style="font-family:">در این مطالعه آزمایشگاهی از 36 رت بالغ نژاد ویستار (8 هفته، 200-250 گرم) استفاده شد. برای القای واریکوسل، ورید کلیوی چپ به صورت نسبی بسته شد. سطح </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">mRNA</span></span> <span b="" lang="FA" mitra="" style="font-family:">ژن&shy;های </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NLRP3</span></span><span b="" lang="FA" mitra="" style="font-family:">،</span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">apoptosis-associated speck-like protein containing a</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">caspase</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">recruitment domain</span></span> <span b="" lang="FA" mitra="" style="font-family:">و </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">caspase-1</span></span> <span b="" lang="FA" mitra="" style="font-family:">با استفاده از</span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">real-time polymerase chain reaction </span></span><span b="" lang="FA" mitra="" style="font-family:">، 1، 2، 4، 8 و 12 هفته بعد از القای واریکوسل بررسی شد.</span><span lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span b="" lang="FA" mitra="" style="font-family:">نتایج: </span></b><span b="" lang="FA" mitra="" style="font-family:">نتایج نشان داد بیان ژن اجزای اینفلامازوم </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NLRP3</span></span> <span b="" lang="FA" mitra="" style="font-family:">شامل </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NLRP3</span></span><span b="" lang="FA" mitra="" style="font-family:">،</span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">apoptosis-associated speck-like protein</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">containing a</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">caspase</span></span><span b="" lang="FA" mitra="" style="font-family:">،</span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> recruitment domain</span></span><span b="" mitra="" style="font-family:"> و </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">caspase-1</span></span><span b="" mitra="" style="font-family:"> تغییر معنی&shy;داری 1، 2، 4 و 8 هفته بعد از جراحی نشان نداد (09/0 </span><span lang="FA" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">= </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span b="" lang="FA" mitra="" style="font-family:">). 12 هفته بعد از القای واریکوسل سطح بیان ژن&shy;ها به صورت معنی&shy;داری افزایش یافت (02/0 </span><span lang="FA" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">= </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span b="" lang="FA" mitra="" style="font-family:">). </span><span lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span b="" lang="FA" mitra="" style="font-family:">نتیجه&shy; گیری:</span></b> <span b="" lang="FA" mitra="" style="font-family:">نتایج ما مدارک روشنی را در خصوص فعال شدن اینفلامازوم&shy;ها 12 هفته بعد از جراحی در بافت بیضه را نشان داد، که این مدت زمان جهت بررسی فعالیت </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NLRP3</span></span> <span b="" lang="FA" mitra="" style="font-family:">در رت مدل واریکوسل الزامی است.</span><span lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Background: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" times="">Varicocele is characterized by abnormal dilation of the testicular vein, which results in hypoxia, the accumulation of reactive oxygen species, and the production of proinflammatory cytokines. It seems that a group of cytosolic receptors named nod-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome, is activated and involved in the pathogenesis of varicocele. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> We aim to determine the time course of NLRP3 inflammasome expression in the testis tissue following varicocele induction.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Materials and Methods:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> In this experimental study, 36 adult Wistar rats (8 wk, 200-250 gr) were used. For the varicocele induction, the left renal vein was partially ligated. The mRNA levels of NLRP3, apoptosis-associated speck-like protein containing a</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> caspase recruitment domain</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">, and caspase-1 were evaluated by real-time polymerase chain reaction at 1, 2, 4, 8, and 12 wk after varicocele induction.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Results: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" times="">Results showed that the gene expression of NLRP3 inflammasome component including NLRP3, apoptosis-associated speck-like protein containing a</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> caspase recruitment domain</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">, and caspase-1 did not alter during week 1, 2, 4, and 8 after operation (p = 0.09). 12 wk after varicocele induction, gene expression levels were significantly up-regulated (p = 0.02).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Conclusion: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" times="">Our data provides clear evidence that varicocele stimulates inflammasome activation in the testis tissue 12 wk after the operation, and this time is required for investigating NLRP3 activity in the varicocele rat model.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></div> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span> واریکوسل, اینفلامازوم, NLRP3, رت. Varicocele, Inflammasomes, NLRP3, Rat. 577 584 http://ijrm.ir/browse.php?a_code=A-10-2038-2&slc_lang=en&sid=1 Hamideh Fallah Asl sogandf70@gmail.com 0000-0002-2912-4053 No Students Research Committee, Arak University of Medical Sciences, Arak, Iran. Farideh Jalali Mashayekhi mashayekhif@yahoo.com 0000-0002-1880-1303 No Department of Genetics and Biochemistry, School of Medicine, Arak University of Medical Sciences, Arak, Iran. Adib Zendedel adib.zendedel@gmail.com 0000-0001-7415-9346 No Institute of Neuroanatomy, Uniklinik RWTH Aachen, 52074 Aachen, Germany. Maryam Baazm dr.baazm@arakmu.ac.ir 0000-0002-1248-6314 Yes Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran. Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran.