en مقایسه سطح سرمی اینترلوکین 33 در همراهی با سطح سرمی CRP و IgG, IgA و IgM در زنان با و بدون از دست رفتن مکرر بارداری: یک مطالعه موردی-شاهدی Fertility & Infertility Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">یکی از دلایل مهم سقط مکرر جنین، عوامل ایمنی است. از این رو یافتن روش&shy;های مناسب پیشگیری یا درمان مؤثر، مستلزم شناخت دلایل آن است.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در این مطالعه، تلاش نمودیم تا سطح سرمی برخی فاکتورهای ایمنولوژیک مرتبط با سقط مکرر را ارزیابی نماییم.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش&shy; ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه به صورت مورد شاهدی از مرداد تا دی ماه 1398، بر روی 66 زن 35-18 ساله مراجعه&shy;کننده به کلینیک زنان و زایمان بیمارستان علی&shy;ابن ابی&shy;طالب (ع) زاهدان، انجام گرفت. سطح سرمی اینترلوکین 33 با روش الایزا اندازه&shy;گیری شد. سطح ایمنوگلوبولین </span></span><span dir="LTR" style="font-size:10.0pt">G</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، ایمنوگلوبولین </span></span><span dir="LTR" style="font-size:10.0pt">A</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، ایمنوگلوبولین </span></span><span dir="LTR" style="font-size:10.0pt">M</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، پروتئین واکنشی </span></span><span dir="LTR" style="font-size:10.0pt">C</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، با روش</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های سرولوژی و هماتولوژی اندازه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">گیری شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">میانگین سن تمامی شرکت&shy;کنندگان در این مطالعه 80/3 </span></span><span lang="FA" style="font-size:10.0pt">&plusmn; </span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">8/30 سال بود. میانگین سرمی اینترلوکین 33 در گروه مورد 1/254</span></span><span lang="FA" style="font-size:10.0pt"> &plusmn; </span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">5/318 پیکوگرم بر میلی&shy;لیتر و کمتر از این میانگین در گروه شاهد، 9/259 </span></span><span lang="FA" style="font-size:10.0pt">&plusmn;</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 2/354 پیکوگرم بر میلی&shy;لیتر بدست آمد. ولی این تفاوت از نظر آماری معنادار نبود (52/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). میانگین سطح سرمی پروتئین واکنشی </span></span><span dir="LTR" style="font-size:10.0pt">C</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، در گروه مورد و شاهد تفاوت معنی&shy;داری نداشت (27/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). بعلاوه در سطح ایمنوگلوبولین </span></span><span dir="LTR" style="font-size:10.0pt">G</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، ایمنوگلوبولین </span></span><span dir="LTR" style="font-size:10.0pt">A</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، در گروه مورد و شاهد تفاوت آماری قابل ملاحظه&shy;ای دیده نشد (به ترتیب 46/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و 16/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، اما سطح سرمی </span></span><span dir="LTR" style="font-size:10.0pt">IgM</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> بین دو گروه مورد مطالعه اختلاف معنی&shy;داری وجود داشت (003/0 </span></span><span dir="LTR" style="font-size:10.0pt">=</span> <span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه &shy;گیری: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به طور کلی سطح سرمی اینترلوکین 33، ایمنوگلوبولین </span></span><span dir="LTR" style="font-size:10.0pt">G</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، ایمنوگلوبولین </span></span><span dir="LTR" style="font-size:10.0pt">A</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و پروتئین واکنشی </span></span><span dir="LTR" style="font-size:10.0pt">C</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> حداقل 6-4 ماه پس از آخرین سقط (در گروه مورد) و آخرین زایمان زنده (در گروه شاهد) در دو گروه تفاوت معناداری نشان نداد. در مقابل سطح سرمی ایمنوگلوبولین </span></span><span dir="LTR" style="font-size:10.0pt">M</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> تفاوت قابل ملاحظه&shy;ای داشت، در نهایت با توجه به نتایج متفاوت متون پیشین در این زمینه، نیاز به مطالعات بیشتر احساس می&shy;گردد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:12pt"><b>Background:</b> One of the critical cases of recurrent pregnancy loss is immunological factors, whereas obtaining effective prevention or treatment is necessary for cognition of reasons.</span><br> <span style="font-size:12pt"><b>Objective:</b> In this study, we tried to evaluate some immunological factors related to<b><i> </i></b>recurrent pregnancy loss.</span><br> <span style="font-size:12pt"><b>Materials and Methods:</b> This case-control study was conducted on 66 women at the age of 18-35 yr who were referred to the Clinic of Gynecology and Obstetrics, Ali Ibn Abi Taleb hospital, Zahedan, Iran, from August-December 2019. Interleukin 33 (IL-33) serum levels were measured using enzyme-linked immunosorbent assay. Immunoglobulin G, Immunoglobulin A, Immunoglobulin M (IgM), and C-reactive protein levels were measured by serology and hematology methods.</span><br> <span style="font-size:12pt"><b>Results:</b> The mean age of total participants was 30.8 &plusmn; 3.80 yr. The mean serum IL-33 in the case group was 318.5&plusmn; &nbsp;254.1 pg/ml and was lower than the control group (354.2&plusmn; &nbsp;259.9 pg/ml), which was not statistically significant (p = 0.52). The level of C-reactive protein in the case and control was not significantly different (p = 0.27), and Immunoglobulin A and Immunoglobulin G in the case and control were also not significantly different) p = 0.46, and p = 0.16, respectively), but there were significant differences (p= &nbsp;0.003) between the level of the IgM in the case and control groups.</span><br> <span style="font-size:12pt"><b>Conclusion:</b> No statistically significant difference was observed in the IL-33 serum level, for at least 4-6 months after the last abortion in the case group and the final live birth in the control group. In contrast, serum levels of IgM were statistically significant. Finally, the need for more studies is felt according to the different results of the previous studies in this field.</span></span></div> اینترلوکین 33, سقط, ایمنوگلوبولین G, ایمنوگلوبولینA, ایمنوگلوبولین M. Interleukin-33, Abortion, IgG, IgA, IgM. 317 322 http://ijrm.ir/browse.php?a_code=A-10-2288-1&slc_lang=en&sid=1 Hossein Ali Khazaei h_khazaei118@yahoo.com 0000-0002-4336-4088 No Clinical Immunology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. Farahnaz Farzaneh f.farzaneh2019@gmail.com 0000-0002-2422-133X No Department of Obstetrics and Gynecology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Saeedeh Sarhadi Dr.sarhadi93@gmail.com 0000-0001-9692-6673 Yes Health Promotion Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. Department of Community Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. Javid Dehghan Haghighi javid_dehghan@yahoo.com 0000-0002-8553-4188 No Department of Community Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. Forough Forghani forghani_f@yahoo.com 0000-0001-7338-3522 No Department of Obstetrics and Gynecology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. Vahid Sheikhi vahid.sheikhie@Gmail.com 0000-0003-3833-6530 No Department of Pediatrics, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. Bahman Khazaei Dr.bahman11@gmail.com 0000-0001-8970-4722 No School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. Lida Asaollahi lida_asadollahi@yahoo.com 0000-0002-6628-8022 No School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.