International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1403
6
1
gregorian
2024
9
1
22
9
online
1
fulltext
en
بیان ژنهای مسیر استروئیدوژنز در سلولهای کومولوس زنان مبتلا به کاهش ذخیره تخمدانی پس از تجویز گنادوتروپین: یک مطالعه مورد-شاهدی
Expression of steroidogenesis pathway genes in cumulus cells from women with diminished ovarian reserve after gonadotropin administration: A case-control study
Reproductive Genetics
Original Article
Original Article
<div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">افراد با کاهش ذخیره تخمدانی (</span></span><span dir="LTR" style="font-size:10.0pt">DOR</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) به داروهای گنادوتروپین پاسخی متفاوت می­دهند.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">این مطالعه با هدف بررسی ارتباط بین بیان ژن­های مؤثر در مسیر استروئیدزایی و پاسخ­دهی به گنادوتروپین در </span></span><span dir="LTR" style="font-size:10.0pt">DOR</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> انجام شد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">مواد و روش­ ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">در این مطالعه مورد-شاهدی، سلولهای کومولوس از زنان مبتلا به </span></span><span dir="LTR" style="font-size:10.0pt">DOR</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> پس از تجویز گنادوتروپین (20 نفر) و ذخیره طبیعی تخمدان (20 نفر) تهیه شد. زنان به 2 گروه با تعداد تخمک کمتر از 3 و بیشتر از 3 تقسیم شدند. پس از استخراج </span></span><span dir="LTR" style="font-size:10.0pt">RNA</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> و سنتز </span></span><span dir="LTR" style="font-size:10.0pt">cDNA</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">،</span></span> <span dir="LTR" style="font-size:10.0pt">qPCR</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> برای ارزیابی سطوح بیان ژنهای آروماتاز سیتوکروم </span></span><span dir="LTR" style="font-size:10.0pt">P450</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> (</span></span><i><span dir="LTR" style="font-size:10.0pt">CYP19A1</span></i><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">)، پروتئین کیناز </span></span><span dir="LTR" style="font-size:10.0pt">A</span><span style="font-size:11.0pt"><span style="font-family:"B Mitra""> (</span></span><i><span dir="LTR" style="font-size:10.0pt">PKA</span></i><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) و گلیکوژن سنتاز کیناز 3 بتا (</span></span><i><span dir="LTR" style="font-size:10.0pt">GSK3B</span></i><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) انجام شد.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتایج نشان داد که زنان مبتلا به </span></span><span dir="LTR" style="font-size:10.0pt">DOR</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> بیان آماری معنیداری کمتری از ژنهای </span></span><i><span dir="LTR" style="font-size:10.0pt">CYP19A1</span></i><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> و </span></span><i><span dir="LTR" style="font-size:10.0pt">PKA</span></i><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> در سلولهای کومولوس خود نسبت به گروه کنترل داشتند (به ترتیب 04/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">، و 001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">). همچنین بیان ژن </span></span><i><span dir="LTR" style="font-size:10.0pt">GSK3B</span></i><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> در </span></span><span dir="LTR" style="font-size:10.0pt">DOR</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> در مقایسه با گروه کنترل کمتر بود، اما معنی­دار نبود. اگرچه بیان ژنهای </span></span><i><span dir="LTR" style="font-size:10.0pt">CYP19A1</span></i><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">، </span></span><i><span dir="LTR" style="font-size:10.0pt">PKA</span></i><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> و </span></span><i><span dir="LTR" style="font-size:10.0pt">GSK3B</span></i><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> در زنان با تخمک کمتر از 3 در مقایسه با زنان با تخمک بیشتر کمتر بود، اما این تفاوت از نظر آماری معنیدار نبود.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتیجه ­گیری: </span></span></b><span dir="LTR" style="font-size:10.0pt">DOR</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> ممکن است با بیان کمتر ژنهای </span></span><i><span dir="LTR" style="font-size:10.0pt">CYP19A1</span></i><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> و </span></span><i><span dir="LTR" style="font-size:10.0pt">PKA</span></i><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> مرتبط باشد. همچنین با توجه به کاهش بیان این ژن­ها در افراد مبتلا به </span></span><span dir="LTR" style="font-size:10.0pt">DOR</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> می</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif"">­</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">توان از بیان این ژن­ها به عنوان ابزاری برای پیش­بینی درمان استفاده کرد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt"><span style="background:white">Background:</span></span></b> <span style="font-size:12.0pt">Women with <a name="_Hlk172967206">diminished </a>ovarian reserve (DOR) respond differently to gonadotropin medications.</span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt">Objective:</span></b><span style="font-size:12.0pt"> This study investigates the relationship between effective gene expression in the steroidogenesis pathway and gonadotropin responsiveness in DOR.</span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><a name="_Hlk173425848"><b><span style="font-size:12.0pt"><span style="background:white">Materials and Methods</span></span></b></a><b><span style="font-size:12.0pt">:</span></b><span style="font-size:12.0pt"> In this </span><span style="font-size:12.0pt">case-control study<i>,</i></span><span style="font-size:12.0pt"> cumulus cells were obtained from women with DOR after gonadotropin administration (n = 20) and normal ovarian reserve (n = 20). They were divided into the following groups,</span><span style="font-size:12.0pt"> oocyte number < 3 and oocyte number > 3. </span><span style="font-size:12.0pt">After RNA extraction and cDNA synthesis, quantitative polymerase chain reaction was performed to assess the expression levels of cytochrome P450 aromatase (<i>CYP19A1</i></span><span style="font-size:12.0pt">)</span><span style="font-size:12.0pt">, protein kinase A<i> </i>(<i>PKA</i>)<i>,</i> and </span><span style="font-size:12.0pt">glycogen synthase kinase 3 beta </span><span style="font-size:12.0pt">(</span><i><span style="font-size:12.0pt">GSK3B</span></i><span style="font-size:12.0pt">)<i> </i>genes.</span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt">Results:</span></b><span style="font-size:12.0pt"> The women with DOR had statistically significant lower expression of <i>CYP19A1</i> and <i>PKA</i> genes in their cumulus cells compared to control group (p = 0.04, and p < 0</span><span style="font-size:12.0pt">.001, respectively). There was also lower expression of the <i>GSK3B</i> gene in DOR compared to control group, but it was not significant. Although the expression of the <i>CYP19A1</i>, <i>PKA</i>, and <i>GSK3B</i> genes was lower in women with < 3 oocytes compared to women with more oocytes, this difference was not statistically significant.</span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt">Conclusion: </span></b><span style="font-size:12.0pt">In conclusion, DOR may be associated with lower expression of <i>CYP19A1</i> and <i>PKA</i> genes. Also, considering the decrease in the expression of these genes in people with DOR, the expression of these genes can be used as a tool to predict the treatment. </span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,"sans-serif""><span style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></div>
تخمدان, ذخیره, استروئیدوژنز, CYP19A1, PKA, GSK3B, گنادوتروپین.
Ovarian, Reserve, Steroidogenesis, CYP19A1, PKA, GSK3B, Gonadotropin.
701
708
http://ijrm.ir/browse.php?a_code=A-10-1558-2&slc_lang=en&sid=1
Zahra
Ahmadnia
zahraahmadnia9@gmail.com