International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1403
3
1
gregorian
2024
6
1
22
6
online
1
fulltext
en
نقش واریانتهای ژنتیکی و بیان بتامیکروسمینوپروتئین در نتیجه لقاح داخل رحمی مردان با ناباروری ناشناخته: یک مطالعه مورد-شاهدی
Role of genetic variations and protein expression of β-Microsemino protein in intrauterine insemination outcome of unexplained infertile men: A case-control study
Reproductive Genetics
Original Article
Original Article
<div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">لقاح داخل رحمی (</span></span><span dir="LTR" style="font-size:10.0pt">IUI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) اغلب خط اول درمان ناباروری با علت نامشخص می­باشد. بتامیکروسمینوپروتئین (</span></span><span dir="LTR" style="font-size:10.0pt">MSMB</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) یکی از پروتئین­های فراوان در پلاسمای منی است که اثر مهاری بر واکنش آکروزوم خودبه­خودی دارد.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف از مطالعه حاضر بررسی تغییرات ژن </span></span><i><span dir="LTR" style="font-size:10.0pt">MSMB</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و بیان پروتئین بر میزان موفقیت </span></span><span dir="LTR" style="font-size:10.0pt">IUI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در مردان نابارور با علت نامشخص بود.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش­ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">یک مطالعه مورد-شاهدی بر روی 100 مرد ایرانی نابارور با علت نامشخص مراجعه­کننده به مؤسسه رویان، تهران، ایران جهت </span></span><span dir="LTR" style="font-size:10.0pt">IUI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> انجام شد (50 بیمار مرد با نتیجه +</span></span><span dir="LTR" style="font-size:10.0pt">IUI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و 50 بیمار مرد با نتیجه </span></span><span dir="LTR" style="font-size:10.0pt">IUI-</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">زوجهای مبتلا به عوامل ناباروری زنانه (مانند اختلالات هورمونی، دوره قاعدگی نادر، ناهنجاری در رحم، لولههای فالوپ یا تخمدانها) و مردان مبتلا به عفونت غدد جانبی مردانه، هیپوگنادیسم هیپوگنادوتروپیک، واریکوسل بالینی، بیضه عقبکشنده، ضربه به دستگاه تناسلی، مصرف مواد مخدر، ریزحذفهای کروموزوم</span></span> <span dir="LTR" style="font-size:10.0pt">Y</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و کاریوتیپ غیرطبیعی یا درمان هورمونی همزمان از مطالعه حذف شدند</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">.</span></span> <span dir="LTR" style="font-size:10.0pt">PCR-Sequencing</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> برای پروموتر و نواحی کدکننده دومین</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های عملکردی </span></span><i><span dir="LTR" style="font-size:10.0pt">MSMB</span></i> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">انجام شد. برای بررسی بیان پروتئین، پروتئین کامل اسپرم استخراج شد و الایزا ساندویچ انجام شد.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">4 واریانت شناسایی شد (</span></span><span dir="LTR" style="font-size:10.0pt">rs12770171</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt">rs10993994</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt">rs2075894</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">rs4517463</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). هیچ یک از آنها تفاوت معنی</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">­</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">داری بین گروه +</span></span><span dir="LTR" style="font-size:10.0pt">IUI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">IUI-</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> نشان ندادند. میانگین بیان پروتئین تفاوتی بین گروه­ها نشان نداد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه­گیری: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بر طبق دادههای ما هیچ ارتباطی بین تغییرات ژنتیکی نواحی پروموتر و نواحی کدکننده دومینهای عملکردی </span></span><i><span dir="LTR" style="font-size:10.0pt">MSMB</span></i> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و همچنین بیان آن با موفقیت </span></span><span dir="LTR" style="font-size:10.0pt">IUI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در مردان نابارور با علت نامشخص وجود ندارد.</span></span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Intrauterine insemination (IUI) is often the first-line treatment for unexplained infertility. </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">β-Microsemino protein </span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">)</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">MSMB</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">) </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">is an abundant protein in seminal plasma that has an inhibitory effect on spontaneous acrosome reaction.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The present study aimed to evaluate <i>MSMB</i> gene variations and protein expression on IUI success rate in unexplained infertile men. </span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> A case-control study was performed on 100 unexplained infertile Iranian men referred to the Royan Institute, Tehran, Iran for IUI (50 men with IUI positive result [IUI+], and 50 men with IUI negative result [IUI-]). </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Couples with female infertility factors (such as hormonal disorders, infrequent menstrual period, abnormality in uterus, fallopian tubes, or ovaries) and </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">men with infections of the male accessory glands, hypogonadotropic hypogonadism, clinical varicocele, retractile testis, genital trauma, drug use, or concurrent hormonal treatment Y chromosome microdeletions, and abnormal karyotype were excluded from the study. The polymerase chain reaction sequencing was performed for the promoter and the coding regions of <i>MSMB</i> functional domains. To study the protein expression, the total protein of sperm was extracted, and sandwich enzyme-linked immunosorbent assay was performed. </span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> 4 variations were detected (rs12770171, rs10993994, rs2075894, and rs4517463). None of them showed significant differences between the IUI+ and IUI- groups. The mean value of protein</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> expression </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">did not show any differences between the groups.</span></span><span style="font-size:12.0pt"><span style="background:white"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> In conclusion, there is no association between genetic variations of promoter and coding regions of <i>MSMB</i> functional domains as well as its expression with IUI success in</span></span><span style="font-size:12.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""> unexplained infertile men</span></span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">.</span></span></span></span></span></span></div>
MSMB, بتامیکروسمینوپروتئین, ناباروری, ناباروری مردان.
MSMB, Beta microseminoprotein, Infertility, Male infertility.
481
494
http://ijrm.ir/browse.php?a_code=A-10-902-1&slc_lang=en&sid=1
Elham
Bagherian
elham69b@gmail.com