en سلول های T ثابت مرتبط با مخاط V𝜶7.2-J𝜶33 انسان در بافت های نابجای آندومتر تمایل به تولید اینترفرون گاما دارند: بازیگری جدید در علت آندومتریوز: یک مطالعه مورد-شاهدی Reproductive Genetics Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">آندومتریوز یک اختلال التهابی مزمن مرتبط با استروژن است که با تکثیر سلول</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های آندومتر در مکانی خارج از رحم شناخته می</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">شود. حضور زیاد&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;سلول</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های ایمنی در مایع صفاقی زنان مبتلا به اندومتریوز، دخالت سیستم ایمنی در پاتوژنز بیماری را تأیید می&shy;کند. سلول&shy;های </span></span><span dir="LTR" style="font-size:10.0pt">MAIT</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">با تولید </span></span><span dir="LTR" style="font-size:10.0pt">IL-17</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt">IFN-&gamma;</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">TNF-&alpha;</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، تأثیر غیرقابل انکاری بر ایمنی مخاط ایفا می</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کنند. عملکرد این سلول</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها در پاتوژنز اندومتریوز کمتر مورد بررسی قرار گرفته است.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف از این مطالعه بررسی نفوذ سلول </span></span><span dir="LTR" style="font-size:10.0pt">MAIT</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با استفاده از تعیین سطح بیان ژن‌های </span></span><i><span dir="LTR" style="font-size:10.0pt">Va7.2-J&alpha;33</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در بافت یوتوپیک و اکتوپیک ضایعات آندومتریوز است.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش&shy; ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در مطالعه مورد-شاهدی، نمونه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های مورد آزمایش شامل 20 بافت اوتوپیک و 20 بافت نابجا زنان مبتلا به اندومتریوز و 20 بافت آندومتر رحم زنان در گروه شاهد بود. بیان ژن</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">های </span></span><i><span dir="LTR" style="font-size:10.0pt">TNF-&alpha;</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><i><span dir="LTR" style="font-size:10.0pt">IL-17A</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><i><span dir="LTR" style="font-size:10.0pt">IFN-&gamma;</span></i><i> </i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">توسط (</span></span><span dir="LTR" style="font-size:10.0pt">q-RT-PCR</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) مورد تجزیه و تحلیل قرار گرفت.</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بر اساس نتایج مطالعه، بیان ژن‌های </span></span><i><span dir="LTR" style="font-size:10.0pt">Va7.2-J&alpha;33</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در رحم و بافت‌های یوتوپیک آندومتر در مقایسه با کنترل داخلی ژن و همچنین در بافت‌های نابجا، افزایش قابل ‌توجهی را نشان نداد. تجزیه و تحلیل همبستگی یک رابطه مثبت بین ژن&shy;های بیان </span></span><i><span dir="LTR" style="font-size:10.0pt">V&alpha;7.2-J&alpha;33</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و سطوح </span></span><i><span dir="LTR" style="font-size:10.0pt">IFN-&gamma;</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در بافت&shy;های نابجا تأیید کرد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه&shy; گیری: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">با توجه به بیان کم ژن اختصاصی سلول‌های </span></span><span dir="LTR" style="font-size:10.0pt">MAIT</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در بافت نابجا، می‌توان نتیجه گرفت که این سلول‌ها به میزان کمی در محیط اندومتریوز حضور دارند و با ترشح </span></span><span dir="LTR" style="font-size:10.0pt">IFN-&gamma;</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> احتمال نقش آنها در پیشرفت اندومتریوز وجود دارد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Endometriosis is a chronic estrogen-related inflammatory disorder that is known by proliferating endometrial cells in a place outside the uterus. The high presence of immune cells in the peritoneal fluid of women with endometriosis confirms the involvement of the immune system in the pathogenesis of the disease. Mucosal-associated invariant T (MAIT) cells play an undeniable impact on mucosal immunity by the production of interleukin-17, interferon-gamma <span style="background:white">(</span>IFN-&gamma;), and tumor necrosis factor-alpha. The function of the cells in the pathogenesis of endometriosis is less investigated. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> This study aims to investigate the infiltration of MAIT cells by using the determination levels of <i>V&alpha;7.2-J&alpha;33</i> gene expression in eutopic and ectopic tissue of endometriosis lesions.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> In this case-control study, the tested samples include 20 eutopic and 20 ectopic tissues of women with endometriosis and 20 uterine endometrial tissues of women in the control group. Expressions of the V&alpha;7.2-J&alpha;33 tumor necrosis factor-alpha, interleukin-17A, and <i>IFN-&gamma;</i> genes were analyzed by quantitative reverse transcriptase-polymerase chain reaction. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> According to the results, <i>V&alpha;7.2-J&alpha;33</i> gene expression did not show substantial elevation in the uterine and eutopic endometrial tissues compared to internal gene control as well as in ectopic tissues. Correlation analysis approved a positive relationship between <i>V&alpha;7.2-J&alpha;33</i> expression genes and <i>IFN-&gamma;</i> levels in ectopic tissues.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Considering the low-expression specific gene of MAIT cells in ectopic tissue, it can be concluded that these cells are present in the endometriotic environment to a certain extent, and there is a possibility of their role in the progression of endometriosis by secreting IFN-&gamma;.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><span dir="RTL" lang="AR-SA" style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><i><span new="" roman="" style="font-family:" times=""> </span></i></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:5.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></div> آندومتریوزیس, MAIT, IFN-γ, TNF-α, TCR V alpha 7.2 J alpha33 , IL-17. Endometriosis, MAIT, IFN-γ, TNF-α, TCR V alpha 7.2-J alpha33, IL-17. 235 244 http://ijrm.ir/browse.php?a_code=A-10-2613-1&slc_lang=en&sid=1 Maryam Zare Moghaddam zmary1371@GMAIL.COM 0000-0002-4429-6400 No Department of Immunology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Fateme Zare Fatemezare91@gmail.com 0000-0003-0436-9924 No Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Reyhane Sandoghsaz Reyhane.sandoghsaz@gmail.com 0000-0001-9224-6443 No Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Abbas Khalil Email:abbas_khalili_30@yahoo.com 0000-0001-9027-5144 No Department of Pediatrics, Shahid Sadoughi Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Ali Shams alis743@yahoo.com 0000-0002-3914-4568 Yes Department of Immunology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.