International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1403
5
1
gregorian
2024
8
1
22
8
online
1
fulltext
en
نقش کربن مونواکساید در اصلاح تغییرات ساختاری تخمدان و کاهش استرس اکسیداتیو از طریق تعدیل سیستم هم-اکسیژناز در مدل موش سندرم تخمدان پلیکیستیک: یک مطالعه تجربی
Carbon monoxide refines ovarian structure changes and attenuates oxidative stress via modulating of heme oxygenase system in a rat model of polycystic ovary syndrome: An experimental study
Reproductive Biology
Original Article
Original Article
<div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کربن مونواکساید (</span></span><span dir="LTR" style="font-size:10.0pt">CO</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">موجود در تخمدان و رحم بر عملکرد تخمدان، بارداری و سلامت جفت تأثیر می­گذارد. هم اکسیژناز (</span></span><span dir="LTR" style="font-size:10.0pt">HO-1</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و محصولات آن، از جمله </span></span><span dir="LTR" style="font-size:10.0pt">CO</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، ویژگی­های محافظتی و ضدالتهابی را نشان می­دهند.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه اثرات محافظتی</span></span> <span dir="LTR" style="font-size:10.0pt">CO</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">که توسط مولکول</span></span> <span dir="LTR" style="font-size:10.0pt">CORM-2</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">آزاد می­شود را در برابر استرس اکسیداتیو، تغییرات عملکردی و ساختاری تخمدان و همچنین میزان بیان پروتیئن</span></span><span dir="LTR" style="font-size:10.0pt">HO-1 </span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در موش­های ماده مبتلا به سندرم تخمدان پلی­کیستیک (</span></span><span dir="LTR" style="font-size:10.0pt">PCOS</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) مورد بررسی قرار داد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش ­ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در این مطالعه تجربی، 24 موش ماده آلبینوس (</span></span><span dir="LTR" style="font-size:10.0pt">wk</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 8، </span></span><span dir="LTR" style="font-size:10.0pt">gr</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 180-200)</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به طور تصادفی به 4 گروه تقسیم شدند (6 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">n</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">):</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کنترل، </span></span><span dir="LTR" style="font-size:10.0pt">CORM-2-PCOS</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و</span></span><span dir="LTR" style="font-size:10.0pt">PCOS+CORM-2</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">. </span></span><span dir="LTR" style="font-size:10.0pt">PCOS</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با تزریق عضلانی و تک دوز استرادیول والرات 4 (</span></span><span dir="LTR" style="font-size:10.0pt">mg/kg</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 4) القا شد. تزریق</span></span> <span dir="LTR" style="font-size:10.0pt">CORM-2</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">با دوز </span></span><span dir="LTR" style="font-size:10.0pt">mg/kg</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 10 به صورت زیرجلدی انجام شد. نمونه­های بافت تخمدان پس از رنگ­آمیزی با کریستال ویولت ارزیابی شدند. سطح مالوندیآلدهید (</span></span><span dir="LTR" style="font-size:10.0pt">MDA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و فعالیت سوپراکساید دیسموتاز (</span></span><span dir="LTR" style="font-size:10.0pt">SOD</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بافت تخمدان با استفاده از تست الایزا ارزیابی شد. بیان </span></span><span dir="LTR" style="font-size:10.0pt">HO-1</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با استفاده از </span></span><span dir="LTR" style="font-size:10.0pt">Western blot</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> ارزیابی شد.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تشکیل</span></span> <span dir="LTR" style="font-size:10.0pt">corpus luteal</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در گروه</span></span> <span dir="LTR" style="font-size:10.0pt">PCOS</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نسبت به گروه کنترل به طور قابل توجهی کاهش یافت. با تزریق </span></span><span dir="LTR" style="font-size:10.0pt">CORM-2</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به گروه</span></span> <span dir="LTR" style="font-size:10.0pt">PCOS</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به میزان قابل توجهی تشکیل جسم زرد افزایش یافت (05/0 </span></span><span dir="LTR" style="font-size:10.0pt"><</span> <span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). میزان بیان پروتئین </span></span> <span dir="LTR" style="font-size:10.0pt">HO-1</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تخمدان در گروه</span></span> <span dir="LTR" style="font-size:10.0pt">PCOS</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نسبت به گروه کنترل کاهش یافت (01/0 </span></span><span dir="LTR" style="font-size:10.0pt">p <</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و تزریق</span></span> <span dir="LTR" style="font-size:10.0pt">CORM</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در موش­های</span></span> <span dir="LTR" style="font-size:10.0pt">PCOS</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به طور قابل توجهی سبب افزایش میزان</span></span> <span dir="LTR" style="font-size:10.0pt">HO-1</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">شد (0001/0 </span></span><span dir="LTR" style="font-size:10.0pt"><</span> <span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">علاوه بر این، استفاده از</span></span> <span dir="LTR" style="font-size:10.0pt">CORM</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به طور قابل توجهی سطح </span></span><span dir="LTR" style="font-size:10.0pt">MDA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> تخمدان را کاهش داد و فعالیت </span></span><span dir="LTR" style="font-size:10.0pt">SOD</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> را افزایش داد (0001/0 </span></span><span dir="LTR" style="font-size:10.0pt"><</span> <span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه­ گیری: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">استفاده از</span></span> <span dir="LTR" style="font-size:10.0pt">CORM-2</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در موش­های</span></span> <span dir="LTR" style="font-size:10.0pt">PCOS</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">با کاهش سطح</span></span> <span dir="LTR" style="font-size:10.0pt">MDA</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و استرس اکسیداتیو، فعالیت</span></span> <span dir="LTR" style="font-size:10.0pt">SOD</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">را زیاد می­کند که سبب افزایش میزان پروتئین </span></span><span dir="LTR" style="font-size:10.0pt">HO-1</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در تخمدان­ها می­شود.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Carbon monoxide (CO), influences ovarian function, pregnancy, and placental health. Heme oxygenase (HO)-1 and its products, including CO, exhibit protective and anti-inflammatory properties.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> This study investigate the protective effects of CO released by the carbon dioxide-releasing molecule (CORM)-2 against oxidative stress, functional and structural changes of the ovaries, and HO-1 expressions in female rats suffering from polycystic ovary syndrome (PCOS). </span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">In this experimental study, 24 <i>Rattus norvegicus var. Albinus</i> female rats (180-200 gr, 8 wk) were randomly divided into 4 groups (n = 6/each): control, CORM-2 (10 mg/kg), PCOS (induced by 4 mg/kg, intramuscular injection and a single dose of estradiol valerate), PCOS + CORM-2. Ovary histological changes were evaluated by</span></span> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">crystal violet staining. Malondialdehyde (MDA) level and superoxide dismutase (SOD) activity of ovarian tissue were assessed using enzyme-linked immunosorbent assay. HO-1 expression was evaluated using Western blot. </span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Corpus luteal formation significantly decreased in the PCOS group and was significantly restored with CORM-2 administration compared to the control group (p < 0.05). The expression of ovarian HO-1 protein was reduced in the PCOS group compared to controls (p < 0.01), and administration of CORM in PCOS rats significantly increased its expression (p < 0.0001). In addition, CORM administration markedly reduced ovarian MDA levels and restored SOD activity (p < 0.0001).</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> CORM-2 administration to PCOS rats created protective effects by reducing oxidative stress (reducing MDA level and restoring SOD activity) and increasing ovarian HO-1 protein.</span></span><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></div>
تخمدان پلیکیستیک, رت, استرس اکسیداتیو, هم اکسیژناز, کورم.
PCOS, Rat, Oxidative stress, Heme oxygenase, CORM.
627
638
http://ijrm.ir/browse.php?a_code=A-10-2623-1&slc_lang=en&sid=1
Bahareh
Asadi
bahareh.asadi13@gmail.com