en ارتباط بیان ژن‌های BAX و BCL-2 اسپرم با نتایج باروری در بیماران مبتلا به الیگواستنوتراتوزوسپرمی تحت تزریق داخل سیتوپلاسمی اسپرم: یک مطالعه مورد-شاهدی Fertility & Infertility Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">پروتئین </span></span><i><span dir="LTR" style="font-size:10.0pt">BCL-2</span></i> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">یکی از اعضای خانواده پروتئین </span></span><i><span dir="LTR" style="font-size:10.0pt">BAX</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> است که به عنوان القاکننده آپوپتوز عمل می&shy;کند.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف از مطالعه حاضر بررسی ارتباط بیان ژن‌های </span></span><i><span dir="LTR" style="font-size:10.0pt">BAX</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:10.0pt">BCL-2</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> اسپرم با نتایج باروری در بیماران تحت تزریق داخل سیتوپلاسمی اسپرم بود.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش&shy;ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مطالعه مورد-شاهدی بر روی 50 مرد انجام شد که به گروه‌های زیر تقسیم شدند: مردان بارور سالم (25 نفر) و مردان نابارور اولیگواستنوتراتوزوسپرمی (25 نفر). آنها تحت گرفتن شرح حال، معاینه بالینی و تجزیه و تحلیل مایع منی قرار گرفتند. بیان ژن‌های </span></span><i><span dir="LTR" style="font-size:10.0pt">BAX</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:10.0pt">BCL-2</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با استفاده از </span></span><span dir="LTR" style="font-size:10.0pt">Real Time-PCR</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> اندازه‌گیری شد. شاخص قطعه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">قطعه شدن </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با استفاده از روش </span></span><span dir="LTR" style="font-size:10.0pt">SCD Assay</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> اندازه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">گیری شد. با استفاده از معیارهای </span></span><span dir="LTR" style="font-size:10.0pt">WHO</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، پارامترهای اسپرم مورد ارزیابی قرار گرفت.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ارزیابی بیان ژن‌های مرتبط با آپوپتوز نشان داد که در بیماران الیگوآستنوتراتوزواسپرمی به طور معنی‌داری بیان </span></span><i><span dir="LTR" style="font-size:10.0pt">mRNA</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> ژن </span></span><i><span dir="LTR" style="font-size:10.0pt">BAX</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> را افزایش یافته و بیان </span></span><span dir="LTR" style="font-size:10.0pt">mRNA</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> ژن </span></span><i><span dir="LTR" style="font-size:10.0pt">BCL-2</span></i><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> را به طور معنی‌داری در مقایسه با کنترل کاهش یافته است. همچنین نسبت </span></span><i><span dir="LTR" style="font-size:10.0pt">BAX/BCL-2</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در الیگوآستنوتراتوزواسپرمی نسبت به گروه کنترل به طور معنی‌داری بالاتر بود (01/0 </span></span><span lang="FA" style="font-size:10.0pt">=</span><span dir="LTR" style="font-size:10.0pt">p</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). همچنین، این مطالعه نشان داد که بیان ژن‌های </span></span><i><span dir="LTR" style="font-size:10.0pt">BAX</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:10.0pt">BCL-2</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با کیفیت اسپرم، آسیب </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در گروه اولیگوآستنوتراتوزوسپرمی همبستگی معنی‌داری داشت (01/0 </span></span><span lang="FA" style="font-size:10.0pt">=</span><span dir="LTR" style="font-size:10.0pt">p</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). در مردان الیگواستنوتراتوزواسپرم میزان قابل توجهی کاهش در میزان لقاح و جنین&shy;های با کیفیت خوب در مرحله تقسیم نسبت به افراد بارور مشاهده شد (01/0 </span></span><span lang="FA" style="font-size:10.0pt">=</span><span dir="LTR" style="font-size:10.0pt">p</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). بین بیان ژن‌های </span></span><i><span dir="LTR" style="font-size:10.0pt">BAX</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:10.0pt">BCL-2</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، لقاح و کیفیت جنین همبستگی معنی‌داری وجود داشت (01/0 </span></span><span lang="FA" style="font-size:10.0pt">=</span><span dir="LTR" style="font-size:10.0pt">p</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه&shy;گیری: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ما به این نتیجه رسیدیم که نسبت </span></span><i><span dir="LTR" style="font-size:10.0pt">BAX/BCL-2</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> اسپرم ارتباط معنی</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">داری را با میزان لقاح و کیفیت جنین نشان می</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">دهد.</span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The B-cell lymphoma 2 (<i>BCL-2</i>) protein is one of the members of the <i>BCL-2 </i>associated X (<i>BAX</i>) protein family that acts as an inducer of apoptosis.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The present study aims to investigate the association between <i>BAX</i> and <i>BCL-2</i> gene expression with reproductive outcome, in cases undergoing intracytoplasmic sperm injection.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></b><span style="font-size:12.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""> In this case-control study, </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">50 men were divided into the healthy fertile and oligoasthenoteratozoospermic infertile men (n = 25/each). They were subjected to history taking, clinical examination, and semen analysis. Expression of <i>BAX</i> and <i>BCL-2</i> genes were measured using real-time polymerase chain reaction. The DNA fragmentation index was measured using the sperm chromatin dispersion assay technique. Using World Health Organization criteria, sperm parameters were evaluated.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Evaluation of apoptosis-related genes showed that oligoasthenoteratozoospermic significantly increased mRNA expression of <i>BAX</i>, and significantly decreased mRNA expression of <i>BCL-2</i>, when compared with control. Moreover, the <i>BAX/BCL-2</i> ratio was significantly higher in oligoasthenoteratozoospermic compared to the normozoospermic group (p = 0.01). Also, this study showed that the <i>BAX</i> and <i>BCL-2</i> genes expression had a significant correlation with sperm quality, and DNA fragmentation in the oligoasthenoteratozoospermic group (p = 0.01). The oligoasthenoteratozoospermic men, had a considerably lower proportion of fertilization rate and good-quality embryos at the cleavage stage than the normozoospermic subjects (p = 0.01). A significant correlation was observed between the expression of <i>BAX</i> and <i>BCL-2</i> genes, fertilization, and embryo quality (p = 0.01).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> We concluded that the sperm <i>BAX/BCL-2</i> ratio demonstrates a significant correlation with fertilization rate and embryo quality.</span></span></span></span></span></div> BAX, BCL-2, لقاح, جنین. BAX, BCL-2, Fertilization, Embryo. 463 472 http://ijrm.ir/browse.php?a_code=A-10-918-1&slc_lang=en&sid=1 Fatemeh Babaei fatemebabaee86@gmail.com 0009-0001-3548-2842 No Biology Department, Islamic Azad University, Qom Branch, Qom, Iran. Maryam Khoshsokhan Muzaffar m.khoshm@gmail.com 0000-0002-1209-1662 No Biology Department, Islamic Azad University, Qom Branch, Qom, Iran. Rahil Jannatifar Rahiljanati2016@gmail.com 0000-0002-6913-4010 Yes Department of Reproductive Biology, Academic Center for Education, Culture, and Research (ACECR), Qom, Iran.