International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
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admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1402
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gregorian
2024
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افزایش فولیکولوژنز و بهبود علایم در مدل ذخیره تخمدانی کاهش یافته در موش Balb/c توسط عصاره دانه گیاه انجره: یک مطالعه تجربی
Urtica pilulifera L. seed extract promotes folliculogenesis and alleviates the diminished ovarian reserve in the Balb/c mice model: An experimental study
Fertility & Infertility
Original Article
Original Article
<div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">بر اساس منابع طب سنتی ایرانی، دانه انجره یا (</span></span><span dir="LTR" style="font-size:10.0pt">UPS</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) </span></span><i><span dir="LTR" style="font-size:10.0pt">Urtica pilulifera </span></i><span dir="LTR" style="font-size:10.0pt">L.</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> اثرات مثبتی بر ناباروری زنان دارد.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">این مطالعه با هدف بررسی اثرات سودمند و مکانیسم زمینه­ای عصاره هیدروالکلی دانه انجره بر روی مدل ذخیره تخمدان کاهش یافته (</span></span><span dir="LTR" style="font-size:10.0pt">DOR</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) القا شده توسط سیکلوفسفامید (</span></span><span dir="LTR" style="font-size:10.0pt">CTX</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) در موش </span></span><span dir="LTR" style="font-size:10.0pt">Balb/c</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> انجام شد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">مواد و روش­ ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">در این مطالعه حیوانی، یک تک دوز داخل صفاقی سیکلوفسفامید ( 75 میلیگرم بر کیلوگرم) برای ایجاد مدل </span></span><span dir="LTR" style="font-size:10.0pt">DOR</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> تجویز شد. 25 موش ماده </span></span><span dir="LTR" style="font-size:10.0pt">Balb/c</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> به طور تصادفی به 5 گروه شامل کنترل سالم (سالین نرمال)، مدل (</span></span><span dir="LTR" style="font-size:10.0pt">DOR</span><span lang="FA" style="font-size:10.0pt">)</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">،</span></span> <span dir="LTR" style="font-size:10.0pt">DOR+50, DOR+100, DOR+200 (mg/kg UPS)</span><span style="font-size:11.0pt"><span style="font-family:"B Mitra""> تقسیم شدند و به­مدت 14 روز گاواژ انجام شد. سطح هورمون­های استروئیدی، نشانگرهای استرس اکسیداتیو، آپوپتوز و تغییرات هیستوپاتولوژیک مورد بررسی قرار گرفت. شناسایی ترکیبات موجود در دانه گزنه با روش </span></span><span dir="LTR" style="font-size:10.0pt">GC/Mass</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> انجام شد.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتایج نشان داد که عصاره انجره </span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""> (</span></span><span dir="LTR" style="font-size:10.0pt">UPS)</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">باعث کاهش غلظت مالون­دی­آلدهید (</span></span><span dir="LTR" style="font-size:10.0pt">MDA</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) و آپوپتوز و همچنین افزایش فعالیت آنزیم سوپراکساید دیسموتاز (</span></span><span dir="LTR" style="font-size:10.0pt">SOD</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">) در مدل </span></span><span dir="LTR" style="font-size:10.0pt">DOR</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> به صورت وابسته به دوز می­شود. علاوه بر این، اثر تعدیل­کننده بر روی هورمون­های استروئیدی مانند </span></span><span dir="LTR" style="font-size:10.0pt">FSH</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">، </span></span><span dir="LTR" style="font-size:10.0pt">LH</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> و </span></span><span dir="LTR" style="font-size:10.0pt">E2</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> اعمال می­کند. تجزیه و تحلیل هیستوپاتولوژیک پتانسیل درمانی عصاره </span></span><span dir="LTR" style="font-size:10.0pt">UPS</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">را نشان داد. ترکیبات شیمیایی اصلی </span></span><span dir="LTR" style="font-size:10.0pt">UPS</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> عبارت بودند از لینولئیک اسید (25/%59)، هگزادکانوییک اسید (36/%10) و اولئیک اسید (29/%8).</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتیجه ­گیری: </span></span></b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتایج نشان داد که عصاره </span></span><span dir="LTR" style="font-size:10.0pt">UPS</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> دارای پتانسیل درمانی در مدل ذخیره تخمدانی کاهش­یافته یا </span></span><span dir="LTR" style="font-size:10.0pt">DOR</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Mitra""> است و یک گزینه درمانی جایگزین ارائه میکند. این پتانسیل به کاهش استرس اکسیداتیو، تعدیل آپوپتوز و تنظیم هورمون­های استروئیدی نسبت داده می­شود که می­تواند با اثرات مفید مشاهده شده از اسیدهای چرب بر بهبود باروری مرتبط باشد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span></div>
<p style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt"><span new="" roman="" times="">Background:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> <i>Urtica pilulifera L.</i> seed (UPS) is a Persian traditional medicine prescription that positively affects female infertility. </span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt"><span new="" roman="" times="">Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""> This study aimed to evaluate the beneficial effects of UPS on a diminished ovarian reserve (DOR) model induced by cyclophosphamide in Balb/c mice.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span class="fontstyle01"><span style="color:#231f20"><span style="font-weight:normal"><span style="font-style:normal"><b><span style="font-size:12.0pt"><span new="" roman="" times="">Materials and Methods:</span></span></b></span></span></span></span><i> </i><span style="font-size:12.0pt"><span new="" roman="" times="">A single intraperitoneal (75 mg/kg) of </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">cyclophosphamide</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> was administered to establish a DOR model. </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">25 female Balb/c mice (6-8 wk, 25 ± 2 gr) were randomly divided into 5 groups (n = 5/each)</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">, including</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> control (normal saline), model (DOR), DOR+50, DOR+100, and DOR+200 (mg/kg UPS, gavage) groups for 14 days. The levels of </span></span><span lang="EN" style="font-size:12.0pt"><span new="" roman="" times="">follicle-stimulating hormone</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">, luteinizing hormone, estradiol, malondialdehyde,</span></span><span lang="EN" style="font-size:12.0pt"><span new="" roman="" times=""> superoxide dismutases</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">, apoptosis, and histopathological alterations were analyzed. </span></span><span lang="EN" style="font-size:12.0pt"><span new="" roman="" times="">Gas chromatography-mass spectrometry </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">analysis performed to identify the phytochemicals </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">of</span></span> <span style="font-size:12.0pt"><span new="" roman="" times="">the </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">UPS.</span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt"><span new="" roman="" times="">Results:</span></span></b><i> </i><span style="font-size:12.0pt"><span new="" roman="" times="">It was observed that </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">the </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">UPS extract </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">reduced </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">malondialdehyde concentration and apoptosis in the DOR model</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> as well as enhanced</span></span> <span lang="EN" style="font-size:12.0pt"><span new="" roman="" times="">superoxide dismutases</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> activity in </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">the </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">ovaries</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> in a dose-dependent manner.</span></span> <span style="font-size:12.0pt"><span new="" roman="" times="">Moreover, it exerted a modulatory effect on</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> steroidal hormones such as </span></span><span lang="EN" style="font-size:12.0pt"><span new="" roman="" times="">follicle-stimulating hormone</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">, luteinizing hormone, and estradiol. The histopathological analysis </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">revealed</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> the therapeutic potential of</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> the</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> UPS extract. The main chemical components of UPS were linoleic acid (59.25%), n-</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">hexadecanoic</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> acid (10.36%), and oleic acid (8.29%).</span></span></span></span><br>
<b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">Conclusion:</span></span></span></b> <span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">The</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times=""> results </span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">indicated that</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times=""> the </span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">UPS extract has </span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">therapeutic potential in the DOR model</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">. This potential is attributed to the reduction of </span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">oxidative stress</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">,</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times=""> modulation</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times=""> of apoptosis</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">, and </span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">regulation of </span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">steroidal </span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">hormones that may</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times=""> be </span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">associated with the observed </span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">beneficial effects of fatty acids </span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times="">on</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times=""> fertility</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" times=""> improvement</span></span></span></span></p>
آپوپتوز, اسیدهای چرب, ناباروری زنان, داروهای گیاهی, طب ایرانی, استرس اکسیداتیو.
Apoptosis, Fatty acids, Female infertility, Herbal medicine, Persian medicine, Oxidative stress.
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http://ijrm.ir/browse.php?a_code=A-10-2632-1&slc_lang=en&sid=1
Sharareh
Hekmat
hekmat7193@yahoo.com