International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1403
4
1
gregorian
2024
7
1
22
7
online
1
fulltext
en
اثرات استریولوژیک و بیوشیمیایی تیموکینون بر سمیت القا شده توسط نانوذرات نقره در بافت تخمدان موشهای NMRI : یک مطالعه تجربی
Stereological and biochemical effects of thymoquinone on ovarian tissue toxicity induced by silver nanoparticles in NMRI mice: An experimental study
Reproductive Biology
Original Article
Original Article
<div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه:</span></span></b><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> سمیت نانوذرات نقره (</span></span><span dir="LTR" style="font-size:10.0pt">AgNPs</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) در دستگاه تناسلی زنان به اثبات رسیده است. تیموکینون (</span></span><span dir="LTR" style="font-size:10.0pt">TQ</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) یک آنتیاکسیدان طبیعی و جزء فعال زیستی </span></span><i><span dir="LTR" style="font-size:10.0pt">Nigella sativa</span></i><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> است.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف:</span></span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ما اثربخشی </span></span><span dir="LTR" style="font-size:10.0pt">TQ</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> را بر روی بافت تخمدان موش­های ماده به دنبال سمیت القا شده توسط </span></span><span dir="LTR" style="font-size:10.0pt">AgNPs</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> را ارزیابی کردیم.</span></span><b><span dir="LTR" style="font-size:11.0pt"></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش­ ها:</span></span></b><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 24 موش ماده نژاد </span></span><span dir="LTR" style="font-size:10.0pt">NMRI</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">با سن 6-5 هفته­ای و میانگین وزنی 33 گرم</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) به طور تصادفی به 4 گروه (6 موش/در هرگروه) تقسیم شدند: کنترل، </span></span><span dir="LTR" style="font-size:10.0pt">AgNPs</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (500 میلیگرم/کیلوگرم، به صورت گاواژ)، </span></span><span dir="LTR" style="font-size:10.0pt">TQ</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (5/2 میلیگرم/کیلوگرم به صورت تزریق داخل صفاقی) و </span></span><span dir="LTR" style="font-size:10.0pt">TQ+AgNPs</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">. موشها روزانه به مدت 35 روز تیمار شدند. سطوح سرمی مالوندیآلدئید (</span></span><span dir="LTR" style="font-size:10.0pt">MDA</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، ظرفیت آنتیاکسیدانی کل</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (</span></span><span dir="LTR" style="font-size:10.0pt">TAC</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، هورمون لوتئینهکننده و هورمون محرک فولیکولی اندازهگیری شد. برای تخمین تعداد فولیکولها و حجم آنها در مراحل مختلف رشد و ساختار بافت تخمدان از روشهای اپتیکال دایسکتور و استریولوژیک استفاده گردید.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج:</span></span></b><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در گروه </span></span><span dir="LTR" style="font-size:10.0pt">AgNPs</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، غلظت سرمی </span></span><span dir="LTR" style="font-size:10.0pt">TAC</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">(01/</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" mitra="" style="font-family:">0 </span></span><span lang="AR-SA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، هورمون لوتئینهکننده (001/0 </span></span><span lang="AR-SA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، هورمون محرک فولیکولی، حجم جسم زرد (001/0 </span></span><span lang="AR-SA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و تعداد فولیکولها در مقایسه با گروه کنترل به طور معنیداری کاهش یافت. با این وجود، </span></span><span dir="LTR" style="font-size:10.0pt">AgNPs</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> سطح </span></span><span dir="LTR" style="font-size:10.0pt">MDA</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> را به طور معنیداری افزایش داد. در گروه </span></span><span dir="LTR" style="font-size:10.0pt">TQ+AgNPs</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> نسبت به گروه </span></span><span dir="LTR" style="font-size:10.0pt">AgNPs</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، کاهش معنیدار در سطح </span></span><span dir="LTR" style="font-size:10.0pt">MDA</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (001/</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" mitra="" style="font-family:">0 </span></span><span lang="AR-SA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و بهبود قابل</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">توجه در </span></span><span dir="LTR" style="font-size:10.0pt">TAC</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (03/0 </span></span><span lang="AR-SA" style="font-size:10.0pt">=</span> <span dir="LTR" style="font-size:10.0pt">p</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و سطح هورمونها، تعداد فولیکولهای اولیه، پریآنترال و آنترال (04/0 </span></span><span lang="AR-SA" style="font-size:10.0pt">=</span> <span dir="LTR" style="font-size:11.0pt">p</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و حجم جسم زرد (01/0 </span></span><span lang="AR-SA" style="font-size:10.0pt">=</span> <span dir="LTR" style="font-size:11.0pt">p</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) مشاهده گردید.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه ­گیری:</span></span></b> <span dir="LTR" style="font-size:10.0pt">TQ</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با کاهش استرس اکسیداتیو و پراکسیداسیون لیپیدی در بافت تخمدان آسیبدیده با </span></span><span dir="LTR" style="font-size:10.0pt">AgNPs</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، تعداد فولیکولها را بهبود بخشید.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><strong>Background:</strong> The toxicity of silver nanoparticles (AgNPs) has been proven in the female reproductive system. Thymoquinone (TQ) is a natural antioxidant and bioactive component of <i>Nigella sativa</i>.</span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><strong>Objective:</strong> We evaluated the efficacy of TQ on ovarian tissue following toxicity induced by AgNPs in female mice.</span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Materials and Methods</b><b>:</b> 24 female NMRI mice (5-6 wk, an average weight of 33 gr) were randomly divided into 4 groups (n = 6/each): control, AgNPs (500 mg/kg, gavage), TQ (2.5 mg/kg, intraperitoneal injection), and TQ+AgNPs. Mice were treated every day for 35 days. Serum levels of malondialdehyde (MDA), total antioxidant capacity (TAC), luteinizing hormone, and follicle-stimulating hormone were measured. The optical disector and stereological techniques were utilized to estimate the follicular count, their volume at different developmental stages, and the structure of ovarian tissue.</span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Results:</b> In the AgNPs group, the serum concentrations of TAC (p = 0.01), luteinizing hormone (p < 0.001), follicle-stimulating hormone, the volume of corpus luteum (p < 0.001), and the number of follicles decreased significantly compared to the control group. Nevertheless, AgNPs significantly increased the MDA level. In the TQ+AgNPs group compared to the AgNPs group, a significant decrease in MDA level (p < 0.001) and a significant improvement in TAC (p = 0.03), and hormonal levels, the number of primary, preantral, and antral follicles (p = 0.04), and the volume of corpus luteum (p = 0.01) were observed.</span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><strong>Conclusion:</strong> TQ improved the number of follicles by reducing oxidative stress and lipid peroxidation in AgNPs-damaged ovarian tissue.</span></span></div>
نانوذرات, نقره, تیموکینون, تخمدان, موش.
Nanoparticles, Silver, Thymoquinone, Ovary, Mouse.
553
566
http://ijrm.ir/browse.php?a_code=A-10-1561-1&slc_lang=en&sid=1
Seyed Mohammad Ali
Shariatzadeh
s-shariatzadeh@araku.ac.ir