en پتانسیل تشخیصی اسیدهای نوکلئیک جنینی بدون سلول در پیش‌بینی عوارض بارداری: یک مرور سیستماتیک و متاآنالیز بر روی تریزومی، پره‌اکلامپسی و دیابت بارداری Reproductive Genetics Review article Review Article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span b="" lang="FA" mitra="" style="font-family:">مقدمه: </span></b><span b="" lang="FA" mitra="" style="font-family:">مطالعات اخیر ارتباط بین افزایش اسید&shy;نوکلئیک جنینی بدون سلول (</span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">cff</span></span><span b="" lang="FA" mitra="" style="font-family:">) در خون مادر و مشکلات بارداری مانند از دست دادن جنین، پره&shy;اکلامپسی، محدودیت رشد و زایمان زودرس را نشان می&shy;دهد.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span b="" lang="FA" mitra="" style="font-family:">هدف: </span></b><span b="" lang="FA" mitra="" style="font-family:">این مقاله نقش اسیدهای نوکلئیک </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">cff</span></span> <span b="" lang="FA" mitra="" style="font-family:">را به عنوان نشانگرهای تشخیصی بالقوه برای پیش‌بینی و نظارت بر پیشرفت عوارض شدید مرتبط با بارداری ارزیابی می‌کند.</span><b><span dir="LTR" style="font-family:"Arial","sans-serif""></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span b="" lang="FA" mitra="" style="font-family:">مواد و روش &shy;ها: </span></b><span b="" lang="FA" mitra="" style="font-family:">در این مرور</span> <span b="" lang="FA" mitra="" style="font-family:">سیستماتیک و متاآنالیز، پایگاه‌های اطلاعاتی مختلفی جستجو شد. مقاله‌های اصیل که در مورد نقش اسیدهای نوکلئیک </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">cff</span></span><span b="" lang="FA" mitra="" style="font-family:"> در پیش‌بینی عوارض بارداری انجام شده بود، انتخاب گردید. برای تجزیه و تحلیل ناهمگنی و سوگیری انتشار به ترتیب از آزمون </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">I square</span></span><span b="" lang="FA" mitra="" style="font-family:"> و </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">funnel plot</span></span><span b="" lang="FA" mitra="" style="font-family:"> استفاده شد. کیفیت مطالعات با استفاده از چک لیست&shy;های ارزیابی انتقادی برای مطالعات که توسط مؤسسه جوانا بریگز ایجاد شده بود ارزیابی شد.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span b="" lang="FA" mitra="" style="font-family:">نتایج: </span></b><span b="" lang="FA" mitra="" style="font-family:">70 مقاله برای تحلیل کیفی نهایی که بین سال&shy;های 2010 تا 2023 منتشر شده و اکثر مطالعات در ایالات متحده آمریکا و چین انجام شده بود انتخاب شد. اکثر مطالعات بر روی مقدار </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">cff-DNA</span></span> <span b="" lang="FA" mitra="" style="font-family:">(40 </span><span lang="FA" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">= </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n</span></span><span b="" lang="FA" mitra="" style="font-family:">)، و بقیه بر روی </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">microRNA</span></span><span b="" lang="FA" mitra="" style="font-family:"> (18 </span><span lang="FA" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">=</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n</span></span><span b="" lang="FA" mitra="" style="font-family:">)، پیام&shy;رسان </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RNA</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> (</span></span><span b="" lang="FA" mitra="" style="font-family:">11</span><span lang="FA" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> = </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n</span></span><span lang="FA" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">)</span></span><span b="" lang="FA" mitra="" style="font-family:">، و </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RNA</span></span><span b="" lang="FA" mitra="" style="font-family:"> بدون سلول (1 </span><span lang="FA" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">=</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n</span></span><span b="" lang="FA" mitra="" style="font-family:">) انجام شده بود. حساسیت ترکیبی اسیدهای نوکلئیک </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">cff</span></span><span b="" lang="FA" mitra="" style="font-family:"> برای تشخیص تریزومی 9/90 (%100-9/80 </span><span lang="FA" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">:</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">CI</span></span> <span b="" lang="FA" mitra="" style="font-family:">95%) بود. سطح </span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MicroRNA</span></span><span b="" lang="FA" mitra="" style="font-family:"> در شرکت‌کنندگان مبتلا به دیابت بارداری با میانگین استاندارد شده 22/1 (34/3-90/0- </span><span lang="FA" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">:</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">CI</span></span> <span b="" lang="FA" mitra="" style="font-family:">%95) به طور قابل توجهی افزایش یافته بود.</span><b><span dir="LTR" style="font-family:"Arial","sans-serif""></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span b="" lang="FA" mitra="" style="font-family:">نتیجه&shy; گیری: </span></b><span b="" lang="FA" mitra="" style="font-family:">اسیدهای نوکلئیک جنین می‌تواند یک تشخیص غیرتهاجمی دقیق برای پیش‌بینی عوارض جدی دوران بارداری باشد.</span><b><span dir="LTR" style="font-family:"Arial","sans-serif""></span></b></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="background:#fcfcfc"><span style="line-height:normal"><span sans-serif=""><b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times="">Background: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" times="">Recent studies reveal an association between increased cell-free fetal (cff) nucleic acid in maternal blood and pregnancy challenges like loss, pre-eclampsia, growth restriction, and preterm labor.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:#fcfcfc"><span style="line-height:normal"><span sans-serif=""><b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times="">Objective:</span></span></b> <span style="font-size:12.0pt"><span new="" roman="" times="">This article</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> assesses the role of cff nucleic acids as </span></span><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times="">potential diagnostic markers for the prediction and monitoring progression of severe pregnancy-related complications</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:#fcfcfc"><span style="line-height:normal"><span sans-serif=""><b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times="">Materials and Methods:</span></span></b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times=""> In this systematic review and meta-analysis, </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">various databases were searched. Original articles reporting on the role of cff nucleic acids in predicting the</span></span><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times=""> complications of pregnancy </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">were included. </span></span><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times="">I square test and funnel plot were used to analyze heterogeneity and publication bias, respectively. </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">The quality of studies was assessed using the critical appraisal checklists for studies created by the Joanna Briggs Institute.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times="">Results:</span></span></b> <span style="font-size:12.0pt"><span new="" roman="" times="">70 publications were selected for the final qualitative analysis. Articles were published between 2010 and 2023, and most studies were conducted in the USA and China. </span></span><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times="">The majority of studies were conducted on the quantity of cff-DNA </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">(n = 40), and the remaining on microRNA (n = 18), messenger RNA (n = 11), and cell-free RNA (n = 1). T</span></span><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times="">he pooled sensitivity of </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">cff nucleic acids for detecting trisomy </span></span><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times="">was found to be 90.9 (95% CI: 80.9-100%). MicroRNA levels were significantly increased in participants with gestational diabetes mellitus, with a standardized mean difference of 1.22 (95% CI: -0.90-3.34). </span></span><span lang="EN-IN" style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times="">Conclusion:</span></span></b><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times=""> F</span></span><span lang="EN-IN" style="font-size:12.0pt"><span new="" roman="" times="">etal nucleic acids can serve as accurate noninvasive diagnostic tools for predicting serious complications during pregnancy. </span></span></span></span></span></span></div> اسیدهای نوکلئیک بدون سلول, بارداری, پیام‌رسان RNA, MicroRNA, دیابت بارداری. Cell-free nucleic acid, Pregnancy, Messenger RNA, MicroRNA, Gestational diabetes. 111 130 http://ijrm.ir/browse.php?a_code=A-10-2715-1&slc_lang=en&sid=1 Jiut Ram Keshari jiutram@rediffmail.com 0000-0002-0531-3298 Yes Department of Biochemistry, Indira Gandhi Institute of Medical Sciences, Patna, Bihar, India. Pritam Prakash pritamprakash2050@gmail.com 0000-0001-6641-6664 No Department of Biochemistry, Indira Gandhi Institute of Medical Sciences, Patna, Bihar, India. Seema Rani Sinha drsrsinha@gmail.com MBBS, M.D. No Department of Biochemistry, Indira Gandhi Institute of Medical Sciences, Patna, Bihar, India. Prem Prakash drpremprakash01@gmail.com 0009-0005-8850-7512 No Department of General Surgery, Indira Gandhi Institute of Medical Sciences, Patna, Bihar, India. Kirti Rani kirtirani5282@gmail.com 0009-0009-3680-0265 No Department of Biochemistry, Indira Gandhi Institute of Medical Sciences, Patna, Bihar, India. Tarique Aziz tarique.aziz1963@gmail.com 0009-0000-3254-5599 No Department of Biochemistry, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India. Shaily Shilpa shailyshilpa1@gmail.com 0009-0008-6118-185X No Department of Biochemistry, Indira Gandhi Institute of Medical Sciences, Patna, Bihar, India.