en ارزیابی کاربرد شدت نوار ژل الکتروفورز DNA مایع بلاستوسل تکثیرشده به عنوان یک رویکرد کم تهاجمی اضافی در انتخاب جنین: یک مطالعه مقطعی Reproductive Genetics Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">انتخاب جنین برای انتقال در درمان‌های کمک‌باروری اهمیت حیاتی دارد. وجود </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در حفره بلاستوسل در بلاستوسیست&shy;های انسانی به عنوان نتیجه‌ای از ناهنجاری‌های کروموزومی رایج پیش از لانه‌گزینی در نظر گرفته می‌شود.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه به بررسی رابطه بین مقدار </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مایع بلاستوسل (</span></span><span dir="LTR" style="font-size:10.0pt">BF-DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و شدت نوار ژل الکروفورز </span></span><span dir="LTR" style="font-size:10.0pt">BF-DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> تکثیرشده، با در نظر گرفتن اثر وضعیت پلوئیدی بر آن می‌پردازد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش&shy;ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در این مطالعه مقطعی، جنین‌های بلاستوسیست بر اساس نتایج هیبریداسیون ژنومی مقایسه‌ای (</span></span><span dir="LTR" style="font-size:10.0pt">aCGH</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) از طریق نمونه&shy;برداری تروفواکتودرم به دو گروه یوپلوئید و آنوپلوئید تقسیم‌بندی شدند. پس از امتیازدهی مورفولوژیک، نمونه</span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">برداری از مایع بلاستوسل برای تکثیر کل ژنوم انجام شد و سپس شدت نوار و میزان </span></span><span dir="LTR" style="font-size:10.0pt">BF-DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> ارزیابی گردید. رابطه&shy;ی بین مقدار </span></span><span dir="LTR" style="font-size:10.0pt">BF-DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، شدت نوار، وضعیت پلوئیدی مورد تجزیه و تحلیل قرار گرفت.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدار </span></span><span dir="LTR" style="font-size:10.0pt">BF-DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در گروه آنوپلوئید نسبت به گروه یوپلوئید بالاتر بود، اما رابطه معنی&shy;داری بین آنها وجود نداشت (2/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). ما مشاهده کردیم که شدت نوار تحت تأثیر وضعیت پلوئیدی جنین‌ها قرار می‌گیرد، اگرچه این تفاوت از نظر آماری معنی‌دار نبود (059/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). تمام بلاستوسیست‌های آنوپلوئیدی که دچار از دست دادن کروموزوم بودند، نوارهای قوی‌ روی ژل نشان دادند. بین مورفولوژی جنین و پلوئیدی کروموزوم ارتباطی وجود نداشت (8/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه&shy; گیری: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">یافته‌های ما نشان می‌دهد که شدت نوار </span></span><span dir="LTR" style="font-size:10.0pt">BF-DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> تکثیرشده در حال حاضر به‌عنوان یک روش برای ارزیابی کیفیت جنین قبل از لانه‌گزینی کاربرد ندارد. به نظر می&shy;رسد جنین&shy;های دچار از دست دادان کروموزوم، بیشتر مستعد رهاکردن </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> به </span></span><span dir="LTR" style="font-size:10.0pt">BF</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> هستند. عدم ارتباط مورفولوژی جنین با پلوئیدی نیاز به توسعه روش&shy;های کم&shy;تهاجمی غربالگری مبتنی بر وضعیت کروموزومی را نشان می&shy;دهد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span></div> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Embryo selection for transfer is critical in assisted reproduction. The presence of DNA in the blastocoel cavity of human blastocysts is assumed to be a consequence of common preimplantation chromosomal abnormalities.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> This study examined the relationship between the amount of blastocoel fluid (BF) DNA and the band intensity of amplified BF-DNA in gel electrophoresis, considering the influence of ploidy status.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">This cross-sectional study categorizes blastocysts into 2 groups based on the array comparative genomic hybridization results by trophectoderm biopsy -the euploid and aneuploid group. After morphological scoring, a biopsy of BF was performed for whole genome amplification, followed by an assessment of band intensity and BF-DNA quantification. The relationship between BF-DNA levels, band intensity, and ploidy status were analyzed. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The level of BF-DNA was higher in the aneuploid group compared to the euploid group, but the difference was not statistically significant (p = 0.2). We observed that the band intensity was affected by the ploidy status of the embryos, although this difference was not statistically significant (p = 0.059). Notably, electrophoresis band of all embryos with chromosomal loss was strong. No correlation was observed between embryo morphology and chromosomal ploidy (p = 0.8).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Our findings indicate that BF-DNA band intensity on agarose gel is not currently applicable for preimplantation embryo selection. It seems that embryos with chromosomal loss are more prone to DNA release to BF. The disrelation between embryo morphology and ploidy status represents the necessity of minimally invasive embryo screening methods based on ploidy status.&nbsp; </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span> : آنوپلوئیدی, بلاستوسیست, مایع بلاستوسل, جنین انسانی, آزمایش ژنتیکی پیش از لانه‌گزینی. Aneuploidy, Blastocyst, Blastocoel fluid, Human embryo, Preimplantation genetic testing. 907 918 http://ijrm.ir/browse.php?a_code=A-10-2746-1&slc_lang=en&sid=1 Fattaneh Khajehoseini Fa_skh@yahoo.com 0009-0007-1069-6100 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Zahra Noormohammadi marjannm@yahoo.com 0000-0003-3890-9001 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Poopak Eftekhari-Yazdi peyazdi50@gmail.com 0000-0003-0730-2530 No Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Hamid Gourabi gourabi@royaninstitute.org 0000-0001-7277-4898 No Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Reza Pazhoomand pazhoomand@yahoo.com 0000-0002-5300-6233 No Medical Genetics Laboratory, Shiraz Fertility Center, Shiraz, Iran. Shirzad Hosseinishenatal shirzad.hoseini@gmail.com 0000-0002-2453-8767 No Department of Embryology, Shiraz Fertility Center, Shiraz, Iran. Masood Bazrgar mbazrgar@royaninstitute.org 0000-0002-5453-9576 Yes Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.