en اثر محافظتی ملاتونین بر بیضه، کیفیت پارامترهای اسپرم و لقاح آزمایشگاهی در موش به دنبال تجویز آفلاتوکسین B1: یک مطالعه تجربی Reproductive Biology Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">آلودگی غذاها و خوراک دام به آفلاتوکسین </span></span><span dir="LTR" style="font-size:10.0pt">B1</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (</span></span><span dir="LTR" style="font-size:10.0pt">AFB1</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) یک مسئله بهداشت عمومی است. در معرض قرار گرفتن با </span></span><span dir="LTR" style="font-size:10.0pt">AFB1</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> باعث ایجاد استرس اکسیداتیو می&shy;شود و می&shy;تواند باعث آسیب سیستم تولید&shy;مثل در مردان شود. ملاتونین (</span></span><span dir="LTR" style="font-size:10.0pt">MLT</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) یک نورو هورمون است که توسط غده اپیفیز و بیضه تولید می&shy;شود و به عنوان یک آنتی&shy;اکسیدان قوی شناخته می&shy;شود.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف از مطالعه حاضر تعیین اثرات محافظتی </span></span><span dir="LTR" style="font-size:10.0pt">MLT</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> بر تغییرات بافت بیضه، شاخص‌های پارامترهای اسپرم و سنجش باروری آزمایشگاهی در موش‌های تحت درمان با </span></span><span dir="LTR" style="font-size:10.0pt">AFB1</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> است.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش&shy; ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در این مطالعه تجربی، 28 موش نر بالغ (10-8 هفته، 27-25 گرم) به طور تصادفی به 4 گروه کنترل، </span></span><span dir="LTR" style="font-size:10.0pt">MLT</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (20 میلی&shy;گرم بر کیلوگرم در روز، داخل صفاقی)، </span></span><span dir="LTR" style="font-size:10.0pt">AFB1</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (50 میکروگرم بر کیلوگرم در روز، داخل صفاقی) و گروه </span></span><span dir="LTR" style="font-size:10.0pt">MLT+AFB1</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تقسیم شدند. پس از 35 روز، تغییرات بافتی بیضه، پارامترهای کیفیت اسپرم، میزان آسیب </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> اسپرم و نتایج لقاح آزمایشگاهی تا مرحله بلاستوسیست بررسی و مابین گروه‌ها مقایسه شد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج نشان داد که تجویز </span></span><span dir="LTR" style="font-size:10.0pt">AFB1</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> باعث ایجاد تغییرات بافتی در بیضه شده و به طور قابل توجهی پارامترهای اسپرم و باروری آزمایشگاهی (درصد لقاح و تشکیل بلاستوسیست) را در مقایسه با گروه کنترل کاهش داد. علاوه بر این، درصد اسپرم&shy;های نابالغ و اسپرم&shy;های دارای آسیب </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در گروه تحت درمان با </span></span><span dir="LTR" style="font-size:10.0pt">AFB1</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> به طور معناداری (001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) افزایش یافت. درمان با </span></span><span dir="LTR" style="font-size:10.0pt">MLT</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در گروه </span></span><span dir="LTR" style="font-size:10.0pt">MLT+AFB1</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> به میزان معناداری کیفیت بیضه، پارامترهای اسپرم را افزایش داد و میزان لقاح آزمایشگاهی و رشد جنینی را بهبود بخشید.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه &shy;گیری: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این یافته&shy;ها نشان داد که </span></span><span dir="LTR" style="font-size:10.0pt">MLT</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> می&shy;تواند اثرات نامطلوب </span></span><span dir="LTR" style="font-size:10.0pt">AFB1</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> بر کیفیت بافت بیضه، پارامترهای اسپرم، </span></span><span dir="LTR" style="font-size:10.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> اسپرم و نتایج لقاح آزمایشگاهی را کاهش دهد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt">Background: </span></b><span style="font-size:12.0pt">Aflatoxin B1 (AFB1) contamination of foods and animal feeds is a public health issue. Exposure to AFB1 induces oxidative stress and can cause male reproductive toxicity. Melatonin (MLT) is a neuro-hormone produced by the pineal gland and the testis and is known as a potent antioxidant.</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt">Objective:</span></b><span style="font-size:12.0pt"> This study aims to determine the protective effect of MLT on testicular tissue alterations, sperm parameter indexes, and in vitro fertility assays in mice treated with AFB1.</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt">Materials and Methods: </span></b><span style="font-size:12.0pt">In this experimental study, 28 adult male NMRI mice (8-10 wk old, 25-27 gr) were divided randomly into 4 groups: control, MLT (20 mg/kg/day, intraperitoneally), AFB1 (50 &mu;g/kg/day, intraperitoneally) and MLT+AFB1. After 35 consecutive days, testis histological changes, sperm quality parameters, the rate of sperm with DNA damage, and in vitro fertilization outcomes up to the blastocyst stage were surveyed and compared between groups.<b> </b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt">Results: </span></b><span style="font-size:12.0pt">Our results showed that AFB1 administration induced histological alterations in the testis and significantly decreased all the sperm parameters and in vitro fertility (fertilization and blastocyst formation rates) compared to control. Additionally, the percentages of immature sperms and sperms with DNA damage significantly (p < 0.001) increased in the AFB1-treated group. MLT treatment in the MLT+AFB1<b> </b>group significantly increased testis quality and sperm parameters and improved in vitro fertilizaton rate and in vitro embryonic development.</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt">Conclusion:</span></b><span style="font-size:12.0pt"> These findings demonstrated that MLT can compensate for the adverse effects of AFB1 on the quality of testicular tissue, sperm parameters, sperm DNA, and in vitro fertilization outcomes.</span></span></span></span><br> <br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><strong><i><span style="font-weight:normal"></span></i></strong></span></span></span></div> ملاتونین, آفلاتوکسین B1, اسپرم, لقاح, موش. Melatonin, Aflatoxin B1, Sperm, Fertilization, Mice. 185 198 http://ijrm.ir/browse.php?a_code=A-10-162-2&slc_lang=en&sid=1 Maryam Sabahi sabahimaryam@gmail.com 0009-0004-0171-0722 No Department of Anatomical Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran. Mojtaba Karimipour mojtaba_karimipour@yahoo.com 0000-0002-2834-2892 Yes Department of Anatomical Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran. Abbas Ahmadi ahmadiabbas36@yahoo.com 0000-0002-3201-5480 No Laboratory of Embryology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. Bagher Pourheydar bagher.pourheydar@gmail.com 0000-0001-9823-6887 No Department of Anatomical Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran. Gholamhossein Farjah hfarjah@hotmail.com 0000-0002-5946-0904 No Department of Anatomical Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.