en بررسی پتانسیل درمانی بافت بیضه محصور شده در غشای آمنیون در مدل موش: یک مطالعه تجربی Reproductive Urology Original Article Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بازگرداندن باروری در افراد سرطانی مرد از طریق پیوند بافت بیضه به دلیل از دست دادن سلول&shy;های بنیادی اسپرماتوگونیال (</span></span><span dir="LTR" style="font-size:10.0pt">SSCs</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ناشی از هیپوکسی با چالش&shy;هایی مواجه است. کپسولاسیون هیدروژل برای به حداقل رساندن آسیب هیپوکسیک در پیوند بافت بیضه مورد بررسی قرار گرفته است. برای این منظور، هیدروژل مشتق از غشای آمنیون انسان (</span></span><span dir="LTR" style="font-size:10.0pt">hAM</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) می&shy;تواند یک جایگزین باشد.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">پتانسیل هیدروژل مشتق شده از آمنیون برای حمایت از پیوند بافت بیضه مورد بررسی قرار گرفت.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش&shy; ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در این مطالعه تجربی، نمونه‌های بافت بیضه (</span></span><span dir="LTR" style="font-size:10.0pt">mm³</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 1-3) از 16 موش نر نژاد </span></span><span dir="LTR" style="font-size:10.0pt">NMRI</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (4-5 هفته، 2 </span></span><span lang="FA" style="font-size:10.0pt">&plusmn;</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 22 گرم) تهیه شد. این قطعات بافتی با یا بدون (کنترل) کپسول کردن در هیدروژل مشتق از پرده آمنیون، بصورت اتولوگ در زیر پوست پشتی موش‌هایی که در معرض اخته یک طرفه یا دوطرفه قرار گرفتند بودند، پیوند شد. بافت‌های بیضه پیوند شده از نظر بافت‌شناسی برای پارامترهای کلیدی، از جمله یکپارچگی لوله‌های منی&shy;ساز (</span></span><span dir="LTR" style="font-size:10.0pt">STs</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، بقای </span></span><span dir="LTR" style="font-size:10.0pt">SSCs</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، عملکرد سلول سرتولی و همچنین هیپوکسی و آپوپتوز در روز 21 از پیوند مورد ارزیابی قرار گرفتند.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تفاوت معنی&shy;داری</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بین گروه&shy;ها از نظر یکپارچگی </span></span><span dir="LTR" style="font-size:10.0pt">ST</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، تعداد </span></span><span dir="LTR" style="font-size:11.0pt"><span arial="" style="font-family:">&shy;</span></span><span dir="LTR" style="font-size:10.0pt">SSC</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ها، عملکرد سلول سرتولی یا سطوح آپوپتوز ناشی از فاکتور 1-آلفا و هیپوکسی مشاهده نشد (05/0</span></span><span dir="LTR" style="font-size:10.0pt">p &le;</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه &shy;گیری: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">این مطالعه هیچ تأثیری از کپسوله کردن بافت&shy;های بیضه در هیدروژل مشتق از پرده آمنیون انسان بر نتایج پیوند بافت بیضه نشان نداد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial","sans-serif""></span></span></b></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt">Background: </span></b><span lang="EN-GB" style="font-size:12.0pt">Restoring fertility in male cancer individuals through testicular tissue transplantation faces challenges due to hypoxia-induced loss of spermatogonial stem cells (SSCs).</span> <span lang="EN-GB" style="font-size:12.0pt">Hydrogel encapsulation was explored to minimize hypoxic damage in testicular tissue transplantation. For this purpose, human amnion membrane (hAM)-derived hydrogel could be an alternative.</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span lang="EN-GB" style="font-size:12.0pt">Objective:</span></b><span lang="EN-GB" style="font-size:12.0pt"> The potential of hAM-derived hydrogel to support testis tissue grafts was evaluated.</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt">Materials and Methods:</span></b><span lang="EN-GB" style="font-size:12.0pt"> In this experimental study, testicular tissue samples (1-3 mm³) were obtained from 16 male NMRI mice (4-5 wk, 22 &plusmn; 2 gr). These tissue fragments were either encapsulated within a hydrogel derived from a hAM or left unencapsulated (control) prior to being autologously transplanted beneath the dorsal skin of mice subjected to hemilateral or bilateral orchiectomy. The grafted testicular tissues were histologically evaluated for key parameters, including the integrity of seminiferous tubules, survival of SSCs, Sertoli cell functionality, as well as hypoxia and apoptosis on day 21.</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt">Results:</span></b><span style="font-size:12.0pt"> No significant differences were observed between groups regarding ST integrity, number of SSCs, Sertoli cell functionality, or the rate of hypoxia-inducible factor 1-alpha and apoptosis (p &le; 0.05).</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:12.0pt">Conclusion:</span></b><span lang="EN-GB" style="font-size:12.0pt"> In conclusion, this study demonstrated no effect of hAM hydrogel encapsulation on the outcomes of testicular tissue transplantation.</span></span></span></span><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></b></span></span></span></div> غشای آمنیوتیک, ECM سلول‌زدایی شده, هیدروژل, کپسولاسیون, بیضه. Amniotic membrane, Decellularized ECM, Hydrogel, Encapsulation, Testis. 171 184 http://ijrm.ir/browse.php?a_code=A-10-2811-1&slc_lang=en&sid=1 Keykavoos Gholami keykavosgholami@yahoo.com 0000-0001-9907-6344 No Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran. 2. Department of Plastic & Reconstructive Surgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA. Elahe Asheghmadine e.asheghmadine99@gmail.com 0009-0001-0654-7715 No Students’ Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran. Fateme Guitynavard f_guitynavard@ymail.com 0000-0002-7664-5329 No Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran. Leila Zareian Baghdadabad l-zareian@farabi.tums.ac.ir 0000-0002-3018-6032 No Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran. Diana Taheri Diana1380@yahoo.com 0000-0002-8131-1120 No Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran. Parisa Zahmatkesh parisa.zhmtksh@gmail.com 0000-0002-7424-0539 No Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran. Leonardo Oliveira Reis Reisleo@unicamp.br 0000-0003-2092-414x No UroScience and Department of Surgery (Urology), School of Medical Sciences, University of Campinas, Unicamp, and Pontifical Catholic University of Campinas, PUC-Campinas, Campinas, São Paulo, Brazil. Seyed Mohammad Kazem Aghamir mkaghamir@tums.ac.ir 0000-0003-1611-0362 Yes Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran.