International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1403
12
1
gregorian
2025
3
1
23
3
online
1
fulltext
en
اثر حفاظتی کرایزین بر بیان ژنهای پروتامین، Tsga10، Dazl و Akap4 در برابر سم دیازینون در موشهای صحرایی نر: یک مطالعه تجربی
Chrysin’s protective effect on the expression of Protamine, Tsga10, Dazl and Akap4 Genes against diazinon toxin in male rats: An experimental study
Fertility & Infertility
Original Article
Original Article
<div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">آلودگی محیط­زیست ناشی از آفت­کش­ها یک مشکل عمده بهداشتی در سراسر جهان است که باعث مسمومیت می­شود. کرایزین (</span></span><span dir="LTR" style="font-size:10.0pt">C</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) یک فلاونوئید است که اثرات نامطلوب ناشی از دیازینون (</span></span><span dir="LTR" style="font-size:10.0pt">D</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) را کاهش می­دهد.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به منظور بررسی اثرات محافظتی کرایزین بر بیان ژن­های <i>پروتامین</i>، آنتی­ژن 10 اختصاصی بیضه</span></span><i> </i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">(</span></span><i><span dir="LTR" style="font-size:10.0pt">Tsga10</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، ژن حذف شده آزواسپرمی (</span></span><i><span dir="LTR" style="font-size:10.0pt">Dazl</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و پروتئین لنگری </span></span><span dir="LTR" style="font-size:10.0pt">A-Kinase 4</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (</span></span><i><span dir="LTR" style="font-size:10.0pt">Akap4</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) در بافت بیضه رت دریافت­کننده </span></span><span dir="LTR" style="font-size:10.0pt">D</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش ­ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در این مطالعه تجربی، 42 سر رت ویستار (8-7 هفته، 250-180 گرم) انتخاب شدند. این مطالعه در سال 2021 در انستیتو پاستور ایران مرکز تحقیقات شمال آمل انجام شد. تزریقات 5 روز در هفته به مدت 4 هفته انجام شد. گروه­بندی تصادفی رت­ها: 1) کنترل: بدون تزریق. 2) شم: تزریق 10% محلول توئین 80 به عنوان حلال</span></span><span dir="LTR" style="font-size:10.0pt">D</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">. 3) </span></span><span dir="LTR" style="font-size:10.0pt">D</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">: تزریق در غلظت </span></span><span dir="LTR" style="font-size:10.0pt">mg/kg/BW</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 20. 4 و 5) </span></span><span dir="LTR" style="font-size:10.0pt">C</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با دوز کم (</span></span><span dir="LTR" style="font-size:10.0pt">mg/kg/BW</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 10 </span></span><span dir="LTR" style="font-size:10.0pt">C</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و دوز بالا (</span></span><span dir="LTR" style="font-size:10.0pt">mg/kg/BW</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 20 </span></span><span dir="LTR" style="font-size:10.0pt">C</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). 6 و 7) </span></span><span dir="LTR" style="font-size:10.0pt">D</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با دوز پایین </span></span><span dir="LTR" style="font-size:10.0pt">C</span><span lang="FA" style="font-size:10.0pt"> (</span><span dir="LTR" style="font-size:10.0pt">mg/kg/BW</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">10 </span></span><span dir="LTR" style="font-size:10.0pt">C</span> <span dir="LTR" style="font-size:10.0pt">mg/kg/BW/</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">20 </span></span><span dir="LTR" style="font-size:10.0pt">D</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) و </span></span><span dir="LTR" style="font-size:10.0pt">D</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با دوز بالای</span></span><span dir="LTR" style="font-size:10.0pt">C</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">: </span></span><span lang="FA" style="font-size:10.0pt">(</span><span dir="LTR" style="font-size:10.0pt">mg/kg/BW</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">20 </span></span><span dir="LTR" style="font-size:10.0pt">C</span> <span dir="LTR" style="font-size:10.0pt">mg/kg/BW/</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">20 </span></span><span dir="LTR" style="font-size:10.0pt">D</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). در نهایت بیضه­ها از نظر بافت­شناسی و بیان ژن مورد بررسی قرار گرفتند.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج بافتشناسی نشان داد که </span></span><span dir="LTR" style="font-size:10.0pt">D</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با کاهش سلولهای ژرمینال، قطر و سطح لومن، باعث بهبود اسپرماتوژنز پس از مداخله با کرایزین میشود. نتایج </span></span><span dir="LTR" style="font-size:10.0pt">SPSS</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> نشان داد که </span></span><span dir="LTR" style="font-size:10.0pt">D</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> بیان ژنهای (0001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) </span></span><i><span dir="LTR" style="font-size:10.0pt">Dazl</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، (01/0 </span></span><span lang="FA" style="font-size:10.0pt">= </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) </span></span><i><span dir="LTR" style="font-size:10.0pt">Tsga10</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و <i>پروتامین</i> (0001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) را کاهش داد، اما بیان ژن </span></span><i><span dir="LTR" style="font-size:10.0pt">Akap4</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> را افزایش داد (0001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">).</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial",sans-serif"></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه­ گیری: </span></span></b><span dir="LTR" style="font-size:10.0pt">D</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بر بیان ژنهای میوزی در بافت بیضه رت­ها اثر منفی دارد، در حالی که کرایزین اثرات نامطلوب ناشی از </span></span><span dir="LTR" style="font-size:10.0pt">D</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">را کاهش میدهد.</span></span><b><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial",sans-serif"></span></span></b></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Background:</b>Environmental pollution with pesticides is a major health problem worldwide that causes toxicity. Chrysin (C) is a flavonoid that reduces the adverse effects caused by diazinon (D).</span></span><br>
<span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b>Objective:</b></span><span new="" roman="" style="font-family:" times="">To investigate the protective effects of Chrysin on the expression of <i>Protamine</i>, <i>Tsga10</i>(Testis-specific gene antigen 10), <i>Dazl (</i><i>deleted azoospermia-like gene)</i><i>,</i> and <i>Akap4</i>(<i>A-Kinase anchoring protein 4</i>) genes in the testicular tissue of rats receiving D.</span></span><br>
<strong>Materials and methods:</strong><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">In this experimental study, 42 Wistar rats(7-8 wk, 180-250 gr) were selected. This study was conducted in<a> </a> 2021 at the Pasteur Institute of Iran, North Amol Research Center. Injections were performed 5 days a week for 4 wk. Random grouping of rats: 1. Control:without any injection. 2. Sham: Injection of 10% Tween 80 solution as D solvent. 3. D: Injection at a concentration of 20 mg/kg/BW. 4 and 5. C with low dose(C10 mg/kg/BW) and high dose(C20 mg/kg/BW). 6 and 7: D with low dose of C (D 20 mg/kg/BW/C10 mg/kg/BW) and D with high dose: </span></span><span style="font-size:12pt"><span new="" roman="" style="font-family:" times="">(D 20 mg/kg/BW/C20 mg/kg/BW). Finally, the testes were examined in terms of histology and gene expression.</span></span><br>
<b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b> <span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Histological results showed that D improved spermatogenesis after C intervention by reducing germinal cells, diameter, and lumen area. SPSS results showed that D reduced the expression </span></span><span style="font-size:12pt"><span new="" roman="" style="font-family:" times="">of <i>D</i><i>azl</i>(p < 0.0001), <i>Tsga10</i>(p = 0.01)<i>,</i> and <i>Protamine</i>(p < 0.0001) genes, but increased the expression of <i>Akap4</i> gene(p < 0.0001).</span></span><br>
<b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b> <span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">D has a negative effect on the expression of meiotic genes in rat testicular tissue,while C greatly reduces the adverse effects caused by D.</span></span></div>
دیازینون, کرایزین, اسپرماتوژنزیس, موش صحرایی نر, بیان ژن.
Diazinon, Chrysin, Spermatogenesis, Rat male. Gene Expression.
275
288
http://ijrm.ir/browse.php?a_code=A-10-2881-1&slc_lang=en&sid=1
Fatemeh
Mojibi
fatemeh.mojibi@gmail.com