International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1404
8
1
gregorian
2025
11
1
23
11
online
1
fulltext
en
بررسی تجربی تأثیر پپتیدهای زیستفعال میگوی سفید بر بهبود عملکرد بیضه در رتهای مبتلا به بیماری کبد چرب غیرالکلی از طریق اتوفاژی، تعادل اکسید و احیا و ناقلهای انرژی
An experimental study on shrimp bioactive peptides restoring testicular function in a rat model of fatty liver disease via autophagy, redox balance, and energy transporters
Cellular and Molecular Biology of Reproduction
Original Article
Original Article
<div style="text-align: justify;"><span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مقدمه: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بیماری کبد چرب غیرالکلی یکی از بیماریهای شایع امروزی است که میتواند باروری و عملکرد دستگاه تولیدمثل را مختل کند.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">هدف این مطالعه بررسی اثرات جیره پرچرب (</span></span><span dir="LTR" style="font-size:10.0pt">HFD</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) بر ساختار بیضه و نقش پپتیدهای زیستفعال حاصل از میگوی پاسفید در کاهش این اثرات بود.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">مواد و روش­ ها: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ضایعات میگوی پاسفید (</span></span><span dir="LTR" style="font-size:10.0pt">HPs</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">با آنزیم آلکالاز در </span></span><span dir="LTR" style="font-size:10.0pt">C</span><sup><span dir="LTR" style="font-size:10.0pt">o</span></sup><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">50 به مدت 3 ساعت هیدرولیز شدند. 24 رت نر ویستار (1/23 </span></span><span lang="FA" style="font-size:10.0pt">±</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 230 گرم، 8 هفته) بهطور تصادفی در 4 گروه (6 حیوان در هر قفس) تقسیم شدند: کنترل، </span></span><span dir="LTR" style="font-size:10.0pt">HFD</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt">HFD+HP20</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">HFD+HP300</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> (جیره پرچرب </span></span><span lang="FA" style="font-size:10.0pt">+</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 20 و 300 </span></span><span dir="LTR" style="font-size:10.0pt">mg/kg BW</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) پس از 10 هفته، بافت بیضه تحت بررسیهای بافتشناسی، مولکولی و بیوشیمیایی قرار گرفت.</span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در گروه </span></span><span dir="LTR" style="font-size:10.0pt">HFD</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، مقادیر </span></span><span dir="LTR" style="font-size:10.0pt">MDA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">GSH</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> افزایش و نسبت </span></span><span dir="LTR" style="font-size:10.0pt">GSH/GSSG</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> حدود 40% کاهش یافت (001/0 </span></span><span lang="FA" style="font-size:10.0pt">> </span><span dir="LTR" style="font-size:10.0pt">p</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">)، که بیانگر استرس احیایی بود. مصرف پپتیدها بهویژه در غلضت بالاتر موجب کاهش استرس، بهبود مورفولوژی لولههای اسپرمساز، و افزایش شاخصهای </span></span><span dir="LTR" style="font-size:10.0pt">TDI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">SPI</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> شد. بیان ژنهای اتوفاژی (</span></span><i><span dir="LTR" style="font-size:10.0pt">Beclin1</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><i><span dir="LTR" style="font-size:10.0pt">Atg7</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><i><span dir="LTR" style="font-size:10.0pt">LC3-I</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><i><span dir="LTR" style="font-size:10.0pt">P62</span></i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) در </span></span><span dir="LTR" style="font-size:10.0pt">HFD</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> افزایش یافت اما با مصرف پپتیدها بیان این ژن ­ها کاهش پیدا کرد. همچنین، کاهش ناقلهای انرژی </span></span><span dir="LTR" style="font-size:10.0pt">GLUT-1</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt">GLUT-3</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt">MCT-4</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در </span></span><span dir="LTR" style="font-size:10.0pt">HFD</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در گروههای دریافتکننده پپتید بازیابی شد.</span></span><span dir="LTR" style="font-size:11.0pt"><span style="font-family:"Arial",sans-serif"></span></span></span></span></span></span></span><br>
<b><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتیجه­گیری: </span></span></b><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">پپتیدهای زیستفعال با بهبود شاخصهای </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TDI</span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPI </span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و بازیابی بیان ناقلهای انرژی در سلولهای سرتولی، حمایت متابولیکی مؤثرتری از سلولهای زایا فراهم کردند. این نتایج نشان میدهد که استفاده از پپتیدهای حاصل از میگو میتواند رویکردی نوین برای بهبود اختلالات تولیدمثلی ناشی از NAFLD</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">N </span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">باشد.</span></span></div>
<div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="line-height:normal"><b><span new="" roman="" times="">Background: </span></b><span new="" roman="" times="">Non-alcoholic fatty liver disease is a widely prevalent condition in the modern era, with potential adverse effects on fertility and the reproductive system.</span><span style="font-family:"Times New Roman",serif"></span></span><br>
<span style="line-height:normal"><b><span new="" roman="" times="">Objective:</span></b><span new="" roman="" times=""> This experimental study investigated the adverse effects of non-alcoholic fatty liver disease induced by a high-fat diet (HFD) on testicular structural integrity, with a focus on oxidative stress and metabolic alterations.</span></span><br>
<span style="line-height:normal"><b><span new="" roman="" times="">Materials and Methods:</span></b><span new="" roman="" times=""> To produce bioactive peptides (HPs), whiteleg shrimp by-products were hydrolyzed using alcalase at 50°C for 3 hr. For this experimental study, 24 male Wistar rats (230 ± 23.1 gr, 8 wk) were randomly assigned to 4 groups (n = 6/each): control (standard diet), HFD-sole, HFD+HP20 (HFD supplemented with 20 mg/kg bodyweight of bioactive peptides), and HFD+HP300 (HFD supplemented with 300 mg/kg BW of bioactive peptides). After 10 wk of dietary intervention, testicular tissues were subjected to histological, molecular, and oxidative stress analyses.</span></span><br>
<span style="line-height:normal"><b><span new="" roman="" times="">Results: </span></b><span new="" roman="" times="">After 70 days, the HFD group showed higher malondialdehyde and glutathione, but a lower glutathione/glutathione disulphide ratio (40%, p < 0.001), indicating reductive stress. HPs, especially at higher doses, alleviated stress, improved seminiferous tubule morphology, and increased tubular differential index and spermiogenesis index indices. Autophagy genes (<i>Beclin1</i>, <i>Atg7</i>, <i>LC3-I</i>, <i>p62</i>) rose in HFD but were downregulated by HPs. Glucose transporter 1, 3 (GLUT-1+, GLUT-3+) and monocarboxylate transporter 4 cell distributions decreased in HFD but were restored in HP-received groups.</span></span><br>
<span style="line-height:normal"><b><span new="" roman="" times="">Conclusion:</span></b><span new="" roman="" times=""> HPs improved tubular differential index and spermiogenesis index values associated with restored GLUT-1/3 and monocarboxylate transporter 4 expression in Sertoli cells, suggesting that Sertoli cells provided enhanced metabolic support for germ cell development.</span></span></span></span></div>
کبد چرب غیرالکلی, پپتیدهای زیستفعال, استرس اکسیداتیو, اتوفاژی, اسپرماتوژنز, بیان ژن, رت.
NAFLD, Bioactive peptides, Oxidative stress, Autophagy, Spermatogenesis, Gene expression, Rat.
937
952
http://ijrm.ir/browse.php?a_code=A-10-2974-1&slc_lang=en&sid=1
Sahar
Javanmard
jvnmrdsahar@gmail.com