International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1392
3
1
gregorian
2013
6
1
11
4
online
1
fulltext
en
تأثیر مواجهه طولانی مدت با سرب بر کیفیت اسپرم مردان مکزیکی
Chronic environmental exposure to lead affects semen quality in a Mexican men population
Original Article
Original Article
<strong><span style="font-family:b mitra;">مقدمه:</span></strong><span style="font-family:b mitra;"> ناباروری در مردان ناشی از عوامل مختلف ایجاد می شود. سرب به عنوان یکی از فلزات سنگین که بیش از میزان تأثیر آن بر اسپرم انسان در معرض آن هستیم یکی از این عوامل می باشد.</span><span dir="LTR"></span><br>
<strong><span style="font-family:b mitra;">هدف: </span></strong><span style="font-family:b mitra;">هدف از انجام این مطالعه بررسی نقش سرب بر کیفیت اسپرم مردان در مواجهه به این فلز بود.</span><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;"></span></span><br>
<strong><span style="font-family:b mitra;">مواد و روش­ها:</span></strong><span style="font-family:b mitra;"> مایع منی و نمونه خون از 2 گروه جمع­آوی گردید. گروهی که در تماس با سرب بودند (20=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">EG</span></span></span><span style="font-family:b mitra;">) و گروهی که در تماس نبودند (27=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">NG</span></span></span><span style="font-family:b mitra;"> .</span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">2</span></span></span><span style="font-family:b mitra;">نمونه مایع منی برای بررسی کیفیت اسپرم و دیگری برای بررسی غلظت سرب استفاده شد. اندازه­گیری میزان سرب مایع منی و خون به وسیله جذب اتمیک اسپکتوفتومتری انجام شد. </span><br>
<strong><span style="font-family:b mitra;">نتایج:</span></strong><span style="font-family:b mitra;"> غلضت سرب خون به طور معنی­داری در گروه </span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">EG</span></span></span><span style="font-family:b mitra;">بالاتر از گروه </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">NEG</span></span></span><span style="font-family:b mitra;"> بود (</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">μgdl-1</span></span></span><span style="font-family:b mitra;"> 10/10</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">0/97در مقابل 0/38</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">6/42) (0/01</span><span style="font-family:b mitra;">></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b mitra;">). همچنین غلظت سرب مایع منی نیز در گروه </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">EG</span></span></span><span style="font-family:b mitra;"> از </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">NEG</span></span></span><span style="font-family:b mitra;"> بالاتر بود (0/35</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">3/28 در مقابل 0/14</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">1/76) (0/043</span><span style="font-family:b mitra;">></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b mitra;">). یک ارتباط معنی­دار بین غلظت سرب مایع منی و غلظت سرب خون در گروه </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">EG</span></span></span><span style="font-family:b mitra;"> پیدا شد (0/05</span><span style="font-family:b mitra;">></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b mitra;"> و 0/573=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">r</span></span></span><span style="font-family:b mitra;">). به طور کلی کیفیت اسپرم در گروه </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">EG</span></span></span><span style="font-family:b mitra;"> از گروه </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">NEG</span></span></span><span style="font-family:b mitra;"> پایین تر بود. تفاوت معنی­دار در غلظت اسپرم (0/01</span><span style="font-family:b mitra;">></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b mitra;"> و </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">cellml-1</span></span></span><span style="font-family:b mitra;"> 10<sup>6</sup></span><span style="font-family:b mitra;">×</span><span style="font-family:b mitra;">8/5</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">68/78=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">NEG</span></span></span><span style="font-family:b mitra;"> و 6/26</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">43/98=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">EG</span></span></span><span style="font-family:b mitra;">)، حرکت اسپرم (0/05</span><span style="font-family:b mitra;">></span><span dir="LTR">p</span><span style="font-family:b mitra;"> و %4</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">67=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">NEG</span></span></span><span style="font-family:b mitra;"> و %7</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">49=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">EG</span></span></span><span style="font-family:b mitra;">)، قابلیت زنده ماندن (0/01</span><span style="font-family:b mitra;">></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b mitra;"> و %1/91</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">72/12=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">NEG</span></span></span><span style="font-family:b mitra;"> و %3/59</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">36/32=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">EG</span></span></span><span style="font-family:b mitra;">) و مورفولوژی غیرطبیعی (0/01</span><span style="font-family:b mitra;">></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b mitra;"> و %12</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">32=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">NEG</span></span></span><span style="font-family:b mitra;"> و %18</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">67=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">EG</span></span></span><span style="font-family:b mitra;">) در بین دو گروه دیده شد. در سلول­های ژرمینال نابالغ (</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">IGC</span></span></span><span style="font-family:b mitra;">) تفاوت در غلظت تنها در سلول­های </span><font face="times new roman, serif" size="2">A</font><span style="font-family:b mitra;"> (100</span><span style="font-family:b mitra;">×</span><span style="font-family:b mitra;">1/9</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">3/2=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">NEG</span></span></span><span style="font-family:b mitra;"> و 100</span><span style="font-family:b mitra;">×</span><span style="font-family:b mitra;">1/1</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">8/1=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">EG</span></span></span><span style="font-family:b mitra;">) </span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">(0/01</span></span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">></span></span></span><span dir="RTL"><span style="font-size:small;"><span style="font-family:times new roman,serif;">p)</span></span></span><span style="font-family:b mitra;"> و سلول­های </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Sab</span></span></span><span style="font-family:b mitra;"> (100</span><span style="font-family:b mitra;">×</span><span style="font-family:b mitra;">1</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">1/1=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">NEG</span></span></span><span style="font-family:b mitra;"> و 100</span><span style="font-family:b mitra;">×</span><span style="font-family:b mitra;">2/2</span><span style="font-family:times new roman,serif;">±</span><span style="font-family:b mitra;">3/4=</span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">EG</span></span></span><span style="font-family:b mitra;">) پیدا شد(0/05</span><span style="font-family:b mitra;">></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b mitra;">). </span><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;"></span></span><br>
<strong><span style="font-family:b mitra;">نتیجه­گیری:</span></strong> <span style="font-family:b mitra;">نتایج می­تواند بیانگر این باشد که تماس طولانی مدت با سرب می­تواند منجر به کاهش کیفیت اسپرم گردد. </span><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;"></span></span>
<strong>Background:</strong> Male infertility is affected by several factors. Lead is one of the heavy metals more bioavailable than usually modifies the sperm quality in humans.<br>
<strong>Objective:</strong> The aim of this study was to establish the role of lead in semen quality in environmentally exposed men.<br>
<strong>Materials and Methods:</strong> Semen and blood samples were obtained from two groups: the exposed group (EG=20) and the non-exposed group (NEG=27). Two semen aliquots were used, one to evaluate spermatic quality and the other for lead determination. Blood (PbB) and semen lead (PbS) determination was performed by atomic absorption spectrophotometry.<br>
<strong>Results:</strong> The PbB concentration was significantly greater in the EG, 10.10±0.97 μgdL-1 than in the NEG, 6.42±0.38 μgdL-1 (p<0.01), as well as the PbS concentration, with 3.28±0.35 and 1.76±0.14μgdL-1 in the EG and NEG respectively (p=0.043). A significant correlation between PbS and PbB concentration in the EG was found (r=0.573, p=0.038). Overall, the spermatic quality was lower in the EG than in the NEG. Specifically, there were significant differences in the spermatic concentration [EG=43.98±6.26 and NEG=68.78±8.51X106 cellmL-1 (p<0.01)], motility [EG=49±7 and NEG=67±4% (p=0.029)], viability [EG=36.32±3.59 and NEG=72.12±1.91% (p<0.01)] and abnormal morphology [EG=67±18 and NEG=32±12% (p<0.01)]. In the immature germ cells (IGC) concentration differences were found only for A cells (EG=8.1±1.1x100 and NEG=3.2±1.9X100 spermatozoa) (p<0.01) and for Sab cells (EG=3.4±2.2x100 and NEG=1.1±1.0X100 spermatozoa) (p=0.041).<br>
<strong>Conclusion: </strong>These results suggest that chronic environmental exposure to low levels of lead adversely affect the spermatic quality.
سرب, کیفیت اسپرم, ناباروری, محیطی.
Lead, Spermatic quality, Infertility, Environmental
267
0
http://ijrm.ir/browse.php?a_code=A-10-1-464&slc_lang=en&sid=1
Javier
Morán-Martínez
javmoran@yahoo.com