International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1392
6
1
gregorian
2013
9
1
11
6
online
1
fulltext
en
روی و کادمیوم با دوز پایین از سمیت کادمیوم با دوز بالا در سلول های سرتولی موشی محافظت می کند: نقش متالوتیونین
Zinc and low-dose of cadmium protect sertoli cells against toxic-dose of cadmium: The role of metallothionein
Original Article
Original Article
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه:</span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> اثرات کادمیوم (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Cd</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) در ایجاد نا باروری مردان ممکن است به میان­کنش این عنصر با پروتئین متصل شونده به فلز دارای وزن مولکولی پایین، متالوتیونین (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Mt</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) ارتباط داشته باشد. همچنین نقش حفاظتی روی (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Zn</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) در مقابل سمیت </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Cd</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> نیز در بیضه ها شناخته شده می­باشد.</span></span><span dir="LTR"><span style="font-size:11.0pt;"></span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف از مطالعه حاضر بررسی تأثیر مواجهه اولیه با </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Zn</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Cd</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با دوز پایین قبل از مواجهه با </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Cd</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با دوز بالا بر میزان بیان ژن­های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Mt1</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Mt2</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در سلول­های سرتولی موشی می­باشد.</span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مواد و روش­ها: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ابتدا سلولهای سرتولی </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">TM4</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> موشی کشت داده شده، به مدت 18 ساعت تحت مواجهه با عناصر </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Zn</span></span></span> (<span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">50</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> میکرومولار، گروه </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">ZnPT</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) یا </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Cd</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> (</span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">2</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> میکرومولار، گروه </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">CdPT</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و یا آب مقطر ( </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">DWPT</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) قرار گرفتند. سپس هر سه گروه برای مدت یک یا دو یا سه و یا شش ساعت با </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Cd</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با دوز بالا (100 میکرو مولار) مواجه شدند. ضمنا یکسری از سلول­ها نیز به عنوان گروه کنترل بعد از مواجهه اولیه با آب مقطر به مدت 18 ساعت، فقط با آب مقطر (به مدت یک یا دو یا سه و یا شش ساعت) مواجه گردیدند.</span></span><span style="font-family:times new roman,serif;"><span style="font-size:12pt;"></span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">میزان بیان </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Mt1</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Mt2</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در گروه­های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">ZnPT</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">CdPT</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و نیز </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">DWPT</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> بالاتر از گروه کنترل بود (به ترتیب0/02=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و0/01=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">). غلظت </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Cd</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> فقط در دو گروه </span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">CdPT</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">DWPT</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با لاتر از گروه کنترل بود (0/00=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">). ضمنا بیان هر دو ژن در گروه­های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">ZnPT</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">CdPT</span></span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">حدود سه ساعت بعد از تیمار به بالاترین سطح خود رسیده و همزمان غلظت </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Cd</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در این گروه­ها کاهش یافت که البته این تغییرات در گروه </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">ZnPT</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> واضح­تر بود.</span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه­گیری:</span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> یافته­های حاصل نشان می­دهد که مواجهه اولیه با </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Zn</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Cd</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با دوز پایین می­تواند از طریق افزایش بیان ژن متالوتیونین در سلول­های سرتولی باعث کاهش اثرات سمی </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Cd</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در مواجهه بعدی گردد که البته نقش روی بارزتر می­باشد.</span></span>
<strong>Background:</strong> The impact of cadmium (Cd) on male infertility may be related to the interaction with metal-binding proteins known as metallothioneins (Mts). Trace elements like zinc (Zn) have protective effects on testicular damage induced by Cd.<br>
<strong>Objective:</strong> We determined the effect of Zn and low-dose Cd pre-treatment on the expression of Mt1 and Mt2 genes on testicular Sertoli cells.<br>
<strong>Materials and Methods:</strong> The cultured TM4 mouse sertoli cells were treated with 50 μM ZnSO4 (Zn pre-treated group; ZnPG), 2 μM CdCl2 (Cd pre-treated group; CdPG), or distilled water (DW pre-treated group; DWPG). After 18 hour, all of these groups were exposed to 100 μM CdCl2 for different periods of time (1, 2, 3, and 6 hours). There was also a control group for all three groups, which was treated only with distilled water (without Cd or Zn pre-treatment). Cellular viability, Zn and Cd concentrations and gene expression were assessed by MTT, atomic absorption spectrometry and real time PCR methods, respectively.<br>
<strong>Results:</strong> The expression of Mt1 and Mt2 genes in ZnPG, CdPG, and DWPG was greater than the control group (p=0.02 and p=0.01, respectively). Cd concentrations in CdPG and DWPG were greater than the control group (p=0.00). Expression of both genes in ZnPG and CdPG increased after 3 hours of treatment and Cd concentration decreased simultaneously, which was more obvious in ZnPG.<br>
<strong>Conclusion:</strong> Zn and short term low-dose Cd pre-treatment might reduce the adverse effects of Cd by increasing expression of Mts genes in Sertoli cells. The protective effect of Zn was stronger than Cd.
کادمیوم, بیان ژن, متالوتیونین, روی, سلول های سرتولی.
Cadmium, Gene expression, Metallothionein, Sertoli cells, Zinc
487
0
http://ijrm.ir/browse.php?a_code=A-10-1-489&slc_lang=en&sid=1
Fatemeh
Kheradmand
فاطمه
خردمند