en شیوع ریزحذف های کروموزوم Y در مردان نابارور پاکستانی Original Article Original Article <strong><span style="font-family:b mitra;">مقدمه: </span></strong><span style="font-family:b mitra;">ریزحذف&shy;های لکوس فاکتور آزواسپرمی بر روی بازوی بلند کروموزوم </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Y</span></span></span><span style="font-family:b mitra;"> یک عامل اینولوژیک اولیگوسپرمی شدید و یا آزوسپرمی می&shy;باشد.</span><br> <strong><span style="font-family:b mitra;">هدف:</span></strong> <span style="font-family:b mitra;">هدف از انجام مطالعه بررسی شیوع ریزحذف&shy;های کروموزوم </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Y</span></span></span><span style="font-family:b mitra;"> در ناحیه </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">AZF</span></span></span><span style="font-family:b mitra;"> و اثر آن بر ناباروری در جمعیت پاکستان بود.</span><span style="font-family:b mitra;"></span><br> <strong><span style="font-family:b mitra;">مواد و روش&shy;ها: </span></strong><span style="font-family:b mitra;">نوع حذف لکوس </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">AZF</span></span></span><span style="font-family:b mitra;"> و اثر آن بر ناباروری در 163 مرد مطالعه شد که 113 نفر نابارور و 50 نفر بارور به&shy;عنوان گروه کنترل بودند. &nbsp;&nbsp;&nbsp;ریزحذف&shy;های کروموزوم </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Y</span></span></span><span style="font-family:b mitra;"> به&shy;وسیله مولتی&shy;پلکس </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">PCR</span></span></span><span style="font-family:b mitra;"> و استفاده از </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">STS 10</span></span></span><span style="font-family:b mitra;"> به نام&shy;های </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">sY81</span></span></span><span style="font-family:b mitra;">، </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">sY130</span></span></span><span style="font-family:b mitra;">، </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">sY141</span></span></span><span style="font-family:b mitra;">، </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">sY142</span></span></span><span style="font-family:b mitra;">، </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">sY155</span></span></span><span style="font-family:b mitra;">، </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">sY157</span></span></span><span style="font-family:b mitra;">، </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">sY160</span></span></span><span style="font-family:b mitra;">، </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">sY182</span></span></span><span style="font-family:b mitra;">، </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">sY231</span></span></span><span style="font-family:b mitra;"> و </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">sY202</span></span></span> <span style="font-family:b mitra;">بود که تمام سه ناحیه </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">AZF</span></span></span><span style="font-family:b mitra;"> را پوشش می&shy;دهند.</span><span style="font-family:b nazanin;"><span style="font-size:14.0pt;"></span></span><br> <strong><span style="font-family:b mitra;">نتایج:</span></strong><span style="font-family:b mitra;"> افراد با اولیگواسپرمی شدید 2/86% شیوع حذف در ناحیه </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">AZFc</span></span></span><span style="font-family:b mitra;"> در مقابل 5/5% در مردان با آزواسپرمی را نشان دادند.</span><br> <strong><span style="font-family:b mitra;">نتیجه&shy;گیری:</span></strong><span style="font-family:b mitra;"> نتایج این مطالعه نشان داد که روش مولتی&shy;پلکس </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">PCR</span></span></span><span style="font-family:b mitra;"> ترجیحا می&shy;تواند برای تشخیص ریزحذف&shy;های کروموزوم </span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Y</span></span></span><span style="font-family:b mitra;"> در مردان نابارور استفاده شود.&nbsp;</span><span style="font-family:arial,sans-serif;"><span style="font-size:14.0pt;"></span></span> <strong>Background:</strong> Microdeletions of the azoospermia factor locus of the long arm of Y chromosome are an etiological factor of severe oligozoospermia or azoospermia.<br> <strong>Objective:</strong> The aim of this study was to investigate the prevalence of Y-chromosome microdeletions in AZF region and their role in infertility in Pakistani population.<br> <strong>Materials and Methods:</strong> The type of deletions in AZF locus were detected in infertile men (n=113) and the association of Y chromosome microdeletions with male infertility was assessed by including men (50) with normal karyotype and having children. Y chromosome microdeletions were detected by multiplex PCR using 10 sequence tagged sites namely sY81, sY130, sY141, sY142, sY155, sY157, sY160, sY182, sY231, and sY202 that covered all three regions of AZF.<br> <strong>Results:</strong> Individuals with severe oligozoospermia showed 2.86% deletion frequency in AZFc region as compared to azoospermic males (5.5%).<br> <strong>Conclusion:</strong> The results of our study showed that deletions in Y chromosome are not playing major part in male infertility. Moreover, multiplex-PCR strategy might preferably be employed for the detection of Y chromosome microdeletions allied to male infertility. ناباروری مردان, آزواسپرمی, لکوس Y کروموزوم, AZF. Male infertility, Azoospermia, Y Chromosome deletions 619 0 http://ijrm.ir/browse.php?a_code=A-10-1-514&slc_lang=en&sid=1 Rubina Tabassum Siddiqui tabassum.rubina@gmail.com Yes Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan Nosheen Mujtaba No Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan Mamoona Naz No Institute of Biochemistry and Biotechnology, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan