en ارتباط فتوئین A، آدینوپکتین، اینترلوکین 10 و ظرفیت کامل آنتی اکسیدان با نتایج IVF Original Article Original Article <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">اثرات احتمالی فاکتورهای ضدالتهابی بعلاوه ظرفیت آنتی اکسیدانی در پروسه تولیدمثل در خانم</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هایی که تحت </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> قرار می</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">گیرند هنوز تحت بررسی است و برخی از نتایج بدست آمده ضد و نقیض می</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">باشد. </span></span><span style="font-family:b nazanin;"></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف مطالعه حاضر بررسی ارتباط بین پارامترهای ضدالتهابی و ظرفیت کامل آنتی اکسیدانی </span></span><span dir="LTR"><span style="font-size:10.0pt;">TAC</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در بدن در حین </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> می باشد. در این رابطه آدینوپکتین، اینترلوکین 10 (</span></span><span dir="LTR"><span style="font-size:10.0pt;">IL-10</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)، گیرنده آنتاگونیست اینترلوکین 1 (</span></span><span dir="LTR"><span style="font-size:10.0pt;">IL-1RA</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) فتوئین </span></span><span dir="LTR"><span style="font-size:10.0pt;">A</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">TAC</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> مورد آنالیز و مطالعه قرار گرفت.</span></span><span dir="LTR"></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مواد و روش&shy;ها:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">در این مطالعه آینده نگر غیر تصادفی و کنترل شده بالینی، سرم از 26 زن بارور (گروه 1) و 26 زن نابارور قبل (گروه 2) و بعد (گروه 3) درمان ناباروری با </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> مورد مطالعه قرار گرفت. </span></span><span dir="LTR"><span style="font-size:10.0pt;">IL-1RA</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، </span></span><span dir="LTR"><span style="font-size:10.0pt;">IL-10</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، فتوئین </span></span><span dir="LTR"><span style="font-size:11.0pt;">A</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، آدینوپکتین و انسولین به وسیله الیزا اندازه گیری شد. </span></span><span dir="LTR"><span style="font-size:10.0pt;">TAC</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> بوسیله اسپتوفتومتر بررسی شد. تست مان ویتنی و </span></span><span dir="LTR"><span style="font-size:10.0pt;">t-test</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و </span></span><span dir="LTR"><span style="font-size:11.0pt;">wilcoxon</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> بعلاوه </span></span><span dir="LTR"><span style="font-size:11.0pt;">pearson</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و نرم افزار </span></span><span dir="LTR"><span style="font-size:11.0pt;">spss</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> جهت مطالعات آماری استفاده شد. </span></span><span style="font-size:11.0pt;"></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">حاملگی بالینی و میزان تولد زنده به ترتیب 30/8% و 23/1% گزارش شد. در زنان باردار یک اثر غیرمستقیم معنی داری بین فتوئین </span></span><span dir="LTR"><span style="font-size:10.0pt;">A</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و آدینوپکتین (0/035=</span></span><span dir="LTR"><span style="font-size:10.0pt;">r</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">=0/843,</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) به همراه (</span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">IL-10</span></span><span dir="RTL"></span><font face="B Mitra"><span style="font-size: 14.545454025268555px;">(r=0/846,p=0/034</span></font><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">&nbsp;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&nbsp;در گروه دو گزارش شد. ارتباط بین آدینوپکتین و </span></span><span dir="LTR"><span style="font-size:10.0pt;">IL-10</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در حاملگی 2 برابر از رنان غیر حامله بود (</span><span style="font-size:14.545454025268555px;">0/033=</span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">r=</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">0/478,</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&nbsp;در برابر 0/007=</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">=0/929,=</span></span><span dir="LTR"><span style="font-size:10.0pt;">r</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) ارتباط بین فتوئین </span></span><span dir="LTR"><span style="font-size:10.0pt;">A</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">TAC</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در خانم های حامله هم در گروه 2 (0/017=</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&nbsp;r=0/892</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و هم در گروه 3 (0/022=</span></span><span dir="LTR"><span style="font-size:10.0pt;">r</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">= 0/875,</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) مشاهده شد. هیچ ارتباطی بین فتوئین </span></span><span dir="LTR"><span style="font-size:10.0pt;">A</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با این متغیرها در خانم</span></span><span dir="LTR"><span style="font-size:11.0pt;">&shy;</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های غیرحامله مشاهده نشد. </span></span><span style="font-family:b nazanin;"><span style="font-size:11.0pt;"></span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه&shy;گیری: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">فتوئین </span></span><span dir="LTR"><span style="font-size:10.0pt;">A</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، </span></span><span dir="LTR"><span style="font-size:10.0pt;">TAC</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ، </span></span><span dir="LTR"><span style="font-size:10.0pt;">IL-10</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">،</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> آدینوپکتین و ازتباط آنها ممکن ایت به عنوان متغیرهای پیش بینی کننده جهت موفقیت </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> مهم باشند. متغیرهای ضدالتهابی و آنتی اکسیدانی به نظر میرسد که باعث موفقیت بیشتر در درمان </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> می&shy;شود.&nbsp;</span></span><br> &nbsp; <strong>Background:</strong> Possible roles of anti-inflammatory factors as well as total antioxidative capacity in reproductive processes of women undergoing in vitro fertilization (IVF) are still being investigated and the contributions by some of them remain controversial.<br> <strong>Objective:</strong> The aim of this study is to investigate the relationship between anti-inflammatory parameters and total antioxidative capacity (TAC) of the body during IVF. In this respect, adiponectin, interleukin-10 (IL-10), interleukin-1 receptor antagonist (IL-1RA), fetuin A and TAC analyses have been performed.<br> <strong>Materials and Methods: </strong>In this prospective, non-randomized, controlled clinical study, sera obtained from 26 fertile (Group-1), and 26 infertile women before (Group-2) and after (Group-3) IVF treatment were analyzed. IL-1RA, IL-10, fetuin A, adiponectin and insulin were determined by ELISA. TAC was determined spectrophotometrically. Mann-Whitney U test, paired sample t-test, Wilcoxon signed-rank test as well as Pearson correlation analysis by SPSS were performed for statistical analysis.<br> <strong>Results:</strong> Clinical pregnancy and live birth rates were determined as 30.8% and 23.1%, respectively, in pregnant group. For the pregnant, significant indirect correlations were detected between fetuin A and adiponectin (r=-0.843; p=0.035) as well as IL-10 (r=-0.846; p=0.034) in Group 2. The correlation between adiponectin and IL-10 doubled in pregnant compared to non-pregnant (r=0.929; p=0.007 vs. r=0.478; p=0.033). The correlations between fetuin A and TAC in pregnant were noted both in Group 2 (r=0.892; p=0.017) and Group 3 (r=0.875; p=0.022). No correlation of fetuin A with these parameters was detected in non-pregnant group.<br> <strong>Conclusion: </strong>Fetuin A, TAC, IL-10, adiponectin and their associations may be important from their predictive values for IVF success point of view. Parameters with anti-inflammatory or antioxidant property appear to improve pregnancy in women undergoing IVF. آدینوپکتین, اینترلوکین 10, فتوئین 10, اینترلوکین RA1, ناباروری. Adiponectin, Interleukin 10, Fetuin A, Interleukin 1RA, Infertility 747 0 http://ijrm.ir/browse.php?a_code=A-10-1-418&slc_lang=en&sid=1 Mustafa Yen No Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey Orkide Donma odonma@gmail.com Yes Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey Ferdag Yildizfer No Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey Ozlem Ekmekci No Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey Asli Karatas Kul No Ministry of Health, Diyarbakir Education and Research Hospital, Diyarbakir, Turkey A. Esat Imal No Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey Zafer Keser No Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey Emin Cagil No Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey Murat Mengi No Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey Hakan Ekmekci No Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey Sezai Sahmay No Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey Metin Donma No Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey