en بیان افتراقی microRNA-143,145 در اندومتریوز Original Article Original Article <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مطالعات اخیر نشان داده است که بیان نامناسب میکرو </span></span><span dir="LTR"><span style="font-size:10.0pt;">RNA</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ها (</span></span><span dir="LTR"><span style="font-size:10.0pt;">miRNA</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) به شدت با وقایع مرتبط با تومورها در انسان در ارتباط است.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف از انجام مطالعه، یافتن تغییرات میکرو </span></span><span dir="LTR"><span style="font-size:10.0pt;">RNA</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ها در اندومتریوز بود.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مواد و روش&shy;ها:</span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">miR-143</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">miR-145</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در اندومترویز یوتوپیک (2=</span></span><span dir="LTR"><span style="font-size:10.0pt;">n</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و اکتوپیک (11=</span></span><span dir="LTR"><span style="font-size:10.0pt;">n</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) (از زنان مبتلا به اندومتریوز) و همچنین اندومتر طبیعی (22 نفر) (از زنان بدون اندومتریوز اما مبتلا به لیومیوما) </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">توسط </span></span><span dir="LTR"><span style="font-size:10.0pt;">real time</span></span> <span dir="LTR"><span style="font-size:10.0pt;">RT-PCR</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> بررسی شد.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">تغییراتی در بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">miR-143</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">miR-145</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">در اندومتر نرمال یا اندومتریوز اکتوپیک همراه با تغییرات سیکل قاعدگی دیده نشد. اما نتایج ما نشان داد که سطح </span></span><span dir="LTR"><span style="font-size:10.0pt;">miR-143</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در اندومتریوط یوتوپیک در مقابل با اکتوپیک افزایش داشته است (0/000=</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">). همچنین سطح </span></span><span dir="LTR"><span style="font-size:10.0pt;">miR-143</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در اندومتریوز یوتوپیک افزایش نشان داد ولی این افزایش معنی دار نبود (0/053=</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">). بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">miR-143</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در اندومتریوز اکتوپیک نسبت به اندومتریوز یوتوپیک افزایش معنی داری را نشان داد</span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">(p=0/016</span></span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">).</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> </span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">miR-145</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&nbsp;خصوصیاتی شبیه </span></span><span dir="LTR"><span style="font-size:10.0pt;">miR-143</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> را نشان داد. </span></span><span dir="LTR"><span style="font-size:10.0pt;">miR-145</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در هر دو بافت اندومتریوز یوتوپیک (0/004=</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و اکتوپیک (0/000=</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) در مقایسه با اندومتر طبیعی افزایش معنی&shy;داری را نشان داد. </span></span><span dir="LTR"><span style="font-size:10.0pt;">miR-145</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در اندومتریوز اکتوپیک در مقایسه با اندومتریوز یوتوپیک افزایش معنی داری داشت. (0/008=</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">).</span></span><span style="font-size:9.0pt;"></span><br> &nbsp;<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه&shy;گیری: </span></span></strong><span dir="LTR"><span style="font-size:10.0pt;">miR-143</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">miR-145</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ممکن است نقش معینی در تکوین و پیشرفت اندومتریوز بازی کنند.&nbsp;</span></span><span dir="LTR"></span> <strong>Background:</strong> Recent studies showed that inappropriate expression of microRNAs (miRNAs) is strongly associated with tumor-related processes in humans (2-9,11-17).<br> <strong>Objective:</strong> To understand the changes of miRNAs in endometriosis.<br> <strong>Materials and Methods:</strong> With real-time RT-PCR, we investigated the miR-143 and miR-145 expression in eutopic (EU, n=2) and ectopic endometrium (EC, n=11) (from women with endometriosis) (as well as EU+EC, n=11), along with the normal endometrium (EN, n=22) (from women without endometriosis, but with leiomyoma).<br> <strong>Results: </strong>We did not find that the expression of miR-143 and/or miR-145 in EN or EC changed with menstrual cycle. But our results showed the miR-143 was up-regulated in EC (p=0.000) compared to EN. The miR-143 was also up-regulated in EU, but the difference did not reach statistically significance (p=0.053). Compared to EU, the expression of miR-143 in EC was up-regulated (p=0.016). MiR-145 had the similar characteristic to miR-143. The miR-145 was up-regulated in both EU (p=0.004) and EC (p=0.000) in compared to EN group. When compared with EU, the miR-145 in EC was up-regulated (p=0.008).<br> <strong>Conclusion:</strong> In conclusion, the miR-143 and miR-145 may play a certain role in the development and progression of endometriosis. RNA (miRNA), miR-143, miR-145, اندومتریوز. MiRNA (microRNA), MiR-143, MiR-145, Endometriosis 555 0 http://ijrm.ir/browse.php?a_code=A-10-1-385&slc_lang=en&sid=1 Bingbing Zheng No Department of Obstetrics, First Affiliated Hospital of Wenzhou Medical University, Zhejiang, China Xiangyang Xue No Department of Microbiology, Wenzhou Medical University, Zhejiang, China Yiping Zhao No Department of Gynecology, Zhuji People's Hospital, Zhejiang, China Jing Chen No Department of Reproductive Medicine, Second Affiliated Hospital of Wenzhou Medical University, Zhejiang, China Chao Yi Xu No Department of Gynecology, Second Affiliated Hospital of Wenzhou Medical University, Zhejiang, China Ping Duan duanping_2011@163.com Yes Department of Obstetrics, First Affiliated Hospital of Wenzhou Medical University, Zhejiang, China