International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1393
10
1
gregorian
2015
1
1
13
1
online
1
fulltext
en
ارتباط بین ناباروری و پلی مورفیسم +331G/A یا progins در ژن گیرنده پروژسترون در یک جمعیت چینی
Association between male infertility and either the +331G/A or the progins polymorphism of the progesterone receptor gene in a Chinese population
Original Article
Original Article
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">پیشنهاد شده که پروژسترون در تنظیم اسپرماتوژنز نقش داشته و تولید اسپرم­های زنده را تسهیل می­کند. پژوهشگران نشان دادند که پلی­مورفیسم گیرنده پروژسترون (</span></span><span dir="LTR"><span style="font-size:10.0pt;">PGR</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) در ارتیاط با برخی از بیماری­ها می­باشد. </span></span><span dir="LTR"></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف از انجام مطالعه، آنالیز ارتباط احتمالی بین ناباروری مردان و پلی­مورفیسم </span></span><span dir="LTR"><span style="font-size:10.0pt;">+331G/A</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">progins</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ژن </span></span><span dir="LTR"><span style="font-size:10.0pt;">PGR</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> بود. </span></span><span dir="LTR"><span style="font-size:11.0pt;"></span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مواد و روش­ها:</span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> این مطالعه در دپارتمان تولیدمثل مردان، مرکز پزشکی تولیدمثل دومین بیمارستان دانشگاه </span></span><span dir="LTR"><span style="font-size:10.0pt;">jilin</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> انجام شد. متد </span></span><span dir="LTR"><span style="font-size:10.0pt;">RFLP</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> برای مشخص کردن جهش­های نقطه­ای ژن استفاده شد. ار 145 نمونه منی آزمایش شده، 35 مورد استنوزسپرمیک، 50 مورد اولیگواستنوزوسپرمیک، 21 مورد آزواسپرمیک، 11 تراتوزوسپرمیک و 28 مورد متعلق به مردان بارور بود.</span></span><strong><span style="font-family:b lotus;"></span></strong><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">آنالیز آماری نشان داد که ژنوتیپ </span></span><span dir="LTR"><span style="font-size:10.0pt;">+331G/A</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در تعادل هاردی-وینبرگ در مردان بارور (534/0=</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، 0=</span></span><span dir="LTR"><span style="font-family:symbol;"><span style="font-size:10.0pt;">c</span></span></span><sup><span dir="LTR"><span style="font-size:10.0pt;">2</span></span></sup><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و گروه اولیگواسپرمیک بود (537/0=</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، 021/0=</span></span><span dir="LTR"><span style="font-family:symbol;"><span style="font-size:10.0pt;">c</span></span></span><sup><span dir="LTR"><span style="font-size:10.0pt;">2</span></span></sup><span style="font-family:b mitra;"><span style="font-size:11.0pt;">). بطور مشابه ژنوتیپ </span></span><span dir="LTR"><span style="font-size:10.0pt;">progins</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> نیز در تعادل هاردی-وینبرگ در مردان بارور (1=</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، 0=</span></span><span dir="LTR"><span style="font-family:symbol;"><span style="font-size:10.0pt;">c</span></span></span><sup><span dir="LTR"><span style="font-size:10.0pt;">2</span></span></sup><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و اولیگواسپرمیک بود (1=</span></span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، 005/0=</span></span><span dir="LTR"><span style="font-family:symbol;"><span style="font-size:10.0pt;">c</span></span></span><sup><span dir="LTR"><span style="font-size:10.0pt;">2</span></span></sup><span style="font-family:b mitra;"><span style="font-size:11.0pt;">). </span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه­گیری: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج ما نشان داد که پلی­مورفیسم </span></span><span dir="LTR"><span style="font-size:10.0pt;">+331G/A</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">progins</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در ژن </span></span><span dir="LTR"><span style="font-size:10.0pt;">PGR</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> بدون ارتباط با ناباروری مردان حداقل در یک جمعیت چینی می­باشد. </span></span><span dir="LTR"><span style="font-size:11.0pt;"></span></span>
<strong>Background: </strong>Progesterone has been suggested to contribute to the regulation of spermatogenesis and to facilitate the production of viable sperm. Investigations have showed that polymorphism of progesterone receptor (PGR) is associated with some diseases.<br>
<strong>Objective:</strong> To analyze the potential relationship between male infertility and the +331G/A and progins polymorphisms of PGR gene.<br>
<strong>Materials and Methods: </strong>The cross-sectional study was carried out at the Department of Male Reproduction, Reproductive Medical Center, the Second Hospital of Jilin University. The restriction fragment length polymorphism (RFLP) technique was used to detect gene point mutations. Of the 145 semen samples analyzed, 35 were asthenozoospermic, 50 were oligoasthenozoospermic, 21 were azoospermic, 11 were teratozoospermic and 28 were from fertile male subjects.<br>
<strong>Results: </strong>Statistical analyses revealed that the genotypes of the +331G/A polymorphisms were in Hardy-Weinberg equilibrium in both the fertile (χ2=0, p=0.534) and oligospermic groups (χ2=0.021, p=0.537). Similarly, the genotypes of the progins polymorphisms were also in Hardy–Weinberg equilibrium in both the fertile (χ2=0, p=1) and oligospermic groups (χ2=0.005, p=1).<br>
<strong>Conclusion: </strong>Our results indicated that polymorphisms of the +331G/A and progins of the PGR gene are unrelated to male infertility, at least in a Chinese population.
گیرنده پروژسترون, پلیمورفیسم +331G/A, پلی مورفیسم progins, ناباروری مردان.
Progesterone receptor, +331G/A polymorphism, Progins polymorphism, Male infertility.
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http://ijrm.ir/browse.php?a_code=A-10-1-276&slc_lang=en&sid=1
Dandan
Li