en بررسی میزان سلولهای کشنده طبیعی CD-56bright و CD-56dim در خون محیطی زنان نابارورکاندید IVF Original Article Original Article <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ناباروری یک مشکل پزشکی و اجتماعی </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">در حال </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">افزایش است. اخیراً، </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> به یک درمان معمول و قابل دسترس برای طیف گسترده</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">ای از این نشانه</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">ها که نتایج متغیری دارند</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">،</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> تبدیل شده است. سلول</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">های کشنده طبیعی به عنوان عوامل ایمنی در خصوص موفقیت یا عدم موفقیت تولیدمثلی شناخته شده</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">اند</span></span><span dir="LTR"><span style="font-size:11.0pt;">.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف از این مطالعه مقایسه درصد سلول</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">های کشنده طبیعی </span></span><span dir="LTR"><span style="font-size:10.0pt;">CD-16</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">CD-56</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در خون محیطی و سطح سلول</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">های کشنده طبیعی در زنان با </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">&nbsp;ناموفق در مقایسه با زنان با </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> موفق بود</span></span><span dir="LTR"><span style="font-size:11.0pt;">.</span></span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:14.0pt;"></span></span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مواد و روش</span></span></strong> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ها: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">سطح</span></span><span dir="LTR"><span style="font-size:10.0pt;">CD56^dim CD16⁺ </span></span>&nbsp;<span style="font-family:b mitra;"><span style="font-size:11.0pt;">و </span></span>&nbsp;<span dir="LTR"><span style="font-size:10.0pt;">CD56^bright CD16^-</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">در 50 زن نابارور تحت درمان با </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> به روش سایتومتری ارزیابی و بین موارد موفق و ناموفق </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> مورد مقایسه قرار گرفت.</span></span><span dir="LTR"><span style="font-family:calibri,sans-serif;"><span style="font-size:14.0pt;"></span></span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج:</span></span></strong> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">در 68 درصد از زنان تحت مطالعه درمان </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ناموفق بوده و 32% آنان </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> موفق داشتند. سطح </span></span><span dir="LTR"><span style="font-size:10.0pt;">CD56^dim CD16⁺</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در زنان با </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ناموفق بطور معنی</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">داری از زنان با </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> موفق بالاتر بود (</span></span><span dir="RTL"></span><font face="B Mitra"><span style="font-size: 15px;">0/0001</span></font><span dir="LTR"><span style="font-size:11.0pt;">p <</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">). سطح </span></span><span dir="LTR"><span style="font-size:10.0pt;">CD56^bright CD16^-</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در زنان با </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ناموفق با زنان دارای </span></span><span dir="LTR"><span style="font-size:10.0pt;">IVF</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> موفق تفاوت معنی</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">داری نداشت</span></span><span dir="RTL"></span><font face="B Mitra"><span style="font-size: 15px;">(0/28=p).</span></font><br> <strong><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه گیری: </span></span></span></strong><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج مطالعه حاضر نشان داد که سطح </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">CD56^dim CD16⁺</span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با عدم حاملگی در زنان با </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">IVF </span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">&nbsp;ناموفق در ارتباط بوده اما ارتباطی بین سطح </span></span></span>&nbsp;<span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">CD56^bright CD16^-</span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در زنان با </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">IVF</span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ناموفق با زنان دارای </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">IVF</span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> موفق وجود نداشت. هرچند انجام مطالعات بیشتر با حجم نمونه بزرگتر ضروری می باشد. پیشنهاد می شود گزینه های درمانی در زنان با </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">IVF</span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ناموفق مکرر </span></span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">با</span></span></span> <span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">افزایش درصد </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">CD56^dim</span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و سطح سلول های کشنده طبیعی در خون محیطی مورد توجه قرار گیرد.</span></span></span> <strong>Background:</strong> Infertility is an increasing medical and social problem. In vitro fertilization (IVF) has become a common and accessible treatment for a wide variety of indications that have variable outcomes. Natural killer (NK) cells have been identified as relevant immunological factors involved in reproductive success or failure.<br> <strong>Objective:</strong> The aim of this study was to compare the percentage of peripheral blood CD56+ (CD56dim and CD56bright) cells and the level of NK cell in patients with IVF failure with those of successful IVF control women.<br> <strong>Materials and Methods:</strong> We assessed the level of CD56dim CD16+ and CD56bright CD16- cells in 50 women under IVF treatment and compared between successful IVF and IVF failure with the flowcytometry technique.<br> <strong>Results:</strong> Of studied women, 68% did not response to IVF therapy and 32% had successful IVF, the level of CD56dim CD16+ cells in women with IVF failure was significantly higher than successful IVF (p<0.0001) but the level of CD56bright CD16- cells was not significantly different between women with IVF failure and successful IVF (p=0.28).<br> <strong>Conclusion: </strong>The results of present study demonstrated that the level of NK cells as a risk factor is associated with pregnancy loss in women with IVF failure. However, number of sample in this study is low and further studies with more sample size are needed to be done. We suggest considering treatment option for women undergoing repeated IVF failure with increased percentage of CD56dim cells and the level of peripheral blood NK cell. سلول کشنده طبیعی, لقاح آزمایشگاهی, ناباروری. Natural killer cells,In vitro fertilization,Infertility 577 582 http://ijrm.ir/browse.php?a_code=A-10-1-203&slc_lang=en&sid=1 Farahnaz Mardanian فرحناز مردانیان No Department of Obstetrics and Gynecology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran گروه زنان و زایمان، دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران Moones Kazeroonizadeh مونس کازرونی زاده energyroham499@gmail.com Yes Department of Obstetrics and Gynecology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran گروه زنان و زایمان، دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران Bahman Rashidi بهمن رشیدی No Department of Anatomy, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran گروه آناتومی، دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران