en Original Article Original Article <strong>Background:</strong> There is mounting evidence for HPV involvement in cervical cancer Human Papilloma Virus DNA is detected by hybridization techniques in 75 &ndash; 100% of patients with condylomas, precancerous cervical dysplasia, and invasive carcinoma.<br> <strong>Objective:</strong> The aim of this study was investigating factors that may contribute to false-negative colposcopic biopsy results in positive high-risk HPV DNA results.<br> <strong>Material and Methods:</strong> Patients positive for high-risk human papillomavirus (HPV) DNA with negative cervical histopathologic findings were examined between January 2004 and August 2006.<br> <strong>Results:</strong> Patients with atypical squamous cells of undetermined significance (ASC) in Papanicolaou smears, with positive HPV DNA results, but negative cervical histopathologic findings accounted for 4.5% of all ASC smears submitted for HPV DNA testing. We found 4% of the cases had focal HPV infection or mild dysplasia. When serial sectioning of the biopsy material were examined, we found that 29% had clinically significant lesions: HPV infection or cervical intraepithelial neoplasia CIN 1, 18%; CIN II/III, 8%; and dysplasia, not otherwise specified (which we can not categorize into any group), 3%. Of the remaining patients, follow-up revealed squamous abnormalities in 25%. About 5% of patients with positive HPV DNA results had a negative follow-up biopsy result. &quot;False-negative&quot; biopsies accounted for one third of cases.<br> <strong>Conclusion: </strong>In almost one third of cases, clinically significant lesions were found when additional levels were examined. Cervix, Dysplasia, Hybrid,Human papilloma virus 99 102 http://ijrm.ir/browse.php?a_code=A-10-549-298&slc_lang=en&sid=1 Ahmad M. Ragab Shalaby ahmad_shalaby20@hotmail.com Yes Department of Oncologic Pathology, Damanhour National Medical Institute, Damanhour, El-Behera, Egypt Ahmad Mostafa Shalaby No Department of Obstetric and Gynecology, Damanhour National Medical Institute, Damanhour, El-Behera, Egypt Saed M. Naguib No Department of Clinical Pathology and Immunology, Damanhour National Medical Institute, Damanhour, El-Behera, Egypt