International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1395
4
1
gregorian
2016
7
1
14
7
online
1
fulltext
en
تاثیر هورمون های استروئیدی بر بیان Oct4 و Sox2 در بافت رحم موش اوریکتومی مدل یائسگی
The effect of steroid hormones on the mRNA expression of oct4 and sox2 in uterine tissue of the ovariectomized mice model of menopause
Original Article
Original Article
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه:</span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> رحم بافتی پویا در طول چرخه تولیدمثلی در پاسخ به تغییرات هورمونی است. به این ترتیب، سلول­های بنیادی رحم در سال­های اخیر مورد مطالعه قرار گرفته است.</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"></span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف:</span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> تحقیق حاضر، بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">Oct4</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">Sox2</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در بافت رحم موش اوریکتومی تیمار شده با هورمون­های استروئیدی را ارزیابی کرد.</span></span><span dir="LTR"><span style="font-size:11.0pt;"></span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مواد و روش­ها:</span></span></strong><span style="font-family: 'b mitra'; font-size: 11pt; "> در این مطالعه تجربی، موش­های ماده باکره اوریکتومی شدند و با استرادیول</span><span style="font-family:cambria,serif;"><span style="font-size:10.0pt;">β</span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> 17</span></span></span><span style="font-family: 'b mitra'; font-size: 11pt; "> </span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)</span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">E<sub>2</sub></span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:11.0pt;">(</span></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، پروژسترون (</span></span><span dir="LTR"><span style="font-size:10.0pt;">P4</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و ترکیب </span></span><span dir="LTR"><span style="font-size:10.0pt;">E<sub>2</sub></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">P4</span></span> <span dir="LTR"><span style="font-size:11.0pt;">(</span></span><span dir="LTR"><span style="font-size:10.0pt;">E<sub>2</sub>&P4</span></span><span dir="LTR"><span style="font-size:11.0pt;">)</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> برای مدت 5 روز تیمار شدند. بافت­های رحم جدا شدند و رنگ آمیزی ایمونوهیستوشیمی (</span></span><span dir="LTR"><span style="font-size:10.0pt;">IHC</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و </span></span><span dir="LTR"><span style="font-size:10.0pt;">PCR</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> کمی زمان واقعی مارکرهای </span></span><span dir="LTR"><span style="font-size:10.0pt;">Oct4</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">Sox2</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> انجام شد.</span></span><span dir="LTR"><span style="font-size:11.0pt;"></span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج:</span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> نتایج </span></span><span dir="LTR"><span style="font-size:10.0pt;">IHC</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> نشان داد که هر دو </span></span><span dir="LTR"><span style="font-size:10.0pt;">Oct4</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">Sox2</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در آندومتر و میومتر رحم تمام گروه</span></span><span dir="LTR"><span style="font-size:11.0pt;">­</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ها بیان شد. بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">Oct4</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">Sox2</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در گروه تیمار شده با </span></span><span dir="LTR"><span style="font-size:10.0pt;">E<sub>2</sub></span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> به طور معنی</span></span><span dir="LTR"><span style="font-size:11.0pt;">­</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">داری در مقایسه با گروه کنترل کاهش یافت. در مقابل، بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">Oct4</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">Sox2</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در گروه های تیمار شده با </span></span><span dir="LTR"><span style="font-size:10.0pt;">P4</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10pt;">E<sub>2</sub>&P4</span></span><span style="font-family:b mitra;"><span style="font-size:11pt;"> تفاوت معنی</span></span><span dir="LTR"><span style="font-size:11pt;">­</span></span><span style="font-family:b mitra;"><span style="font-size:11pt;">داری در مقایسه با گروه کنترل نشان ندادند.</span></span><span dir="LTR"><span style="font-size:11.0pt;"></span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه­گیری: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج نشان می</span></span><span dir="LTR"><span style="font-size:11.0pt;">­</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">دهد هورمون</span></span><span dir="LTR"><span style="font-size:11.0pt;">­</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های استروئیدی تخمدان بیان </span></span><span dir="LTR"><span style="font-size:10.0pt;">Oct4</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">Sox2</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> را در بافت رحم موش تغییر می</span></span><span dir="LTR"><span style="font-size:11.0pt;">­</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">دهد که بیانگر دخالت تنظیم هورمون استروئیدی در سلول</span></span><span dir="LTR"><span style="font-size:11.0pt;">­</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">های بنیادی رحم می</span></span><span dir="LTR"><span style="font-size:11.0pt;">­</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">باشد.</span></span>
<strong>Background:</strong> The uterus is a dynamic tissue responding to hormonal changes during reproductive cycles. As such, uterine stem cells have been studied in recent years. Transcription factors oct4 and sox2 are critical for effective maintenance of pluripotent cell identity. <br>
<strong>Objective: </strong>The present research evaluated the mRNA expression of oct4 and sox2 in the uterine tissues of ovariectomized mice treated with steroid hormones. <br>
<strong>Materials and Methods: </strong>In this experimental study, adult virgin female mice were ovariectomized and treated with estradiol 17β (E2), progesterone (P4), and a combination of E2 and P4 (E2 & P4) for 5 days. Uterine tissues were removed, and immunofluorescent (IF) staining and quantitative real-time PCR of oct4 and sox2 markers were performed. <br>
<strong>Results: </strong>IF showed oct4 and sox2 expression in the uterine endometrium and myometrium among all groups. The mRNA expression of oct4 (p=0.022) and sox2 (p=0.042) in the E2-treated group significantly were decreased compared to that in the control group. By contrast, the mRNA expression of oct4 and sox2 in the P4 (p=0.641 and 0.489 respectively) and E2 & P4-treated groups (p=0.267 and 0.264 respectively) did not show significant differences compared to the control group. <br>
<strong>Conclusion: </strong>The results indicate ovarian steroid hormones change the expression of oct4 and sox2 in the mice uterine tissues, which suggest the involvement of steroid hormonal regulation in uterine stem cells.
موش, استرادیول, پروژسترون, نشانگرهای پرتوان.
Mice, Uterine, Estradiol, Progesterone, stem cells
471
476
http://ijrm.ir/browse.php?a_code=A-10-1-135&slc_lang=en&sid=1
Marzieh
Davoudi
مرضیه
داودی