International Journal of Reproductive BioMedicine International Journal of Reproductive BioMedicine IJRM Medical Sciences http://ijrm.ir 1 admin 2476-4108 2476-3772 10.29252/ijrm en jalali 1386 4 1 gregorian 2007 7 1 5 4 online 1 fulltext
en In vitro application of Matrigel enhances human blastocyst formation and hatching Original Article Original Article <strong>Background:</strong>&nbsp;Matrigel (extracellular matrix) can improve the growth of many cell types <em>in vitro</em>.<br> <strong>Objective:</strong> The aim of the present study was to determine the effect of Matrigel on the development of 2-4 cells human embryos in culture.<br> <strong>Material and Methods:</strong> Surplus 2-4 cells human embryos, resulting from ICSI, were divided into two groups (control and test). Quality of embryos in both groups was morphologically similar. The test group (n=140) was cultured in Hams&rsquo; F10 supplemented with 10% human serum albumin and 150 &micro;l liquid Matrigel. The control group (n=140) was cultured in the same medium devoid of Matrigel. Embryos were cultured for an additional 4 days and their morphology was assessed every 24 hours. Both groups were then statistically compared.<br> <strong>Results:</strong> The percentage of the human embryos that reached the morula stage in the control and test groups were 79.2% and 80%, respectively (p>0.05).&nbsp; However, 36.4% of embryos reached the blastocyst stage in the test group as compared to 5.7% in the control group after 144 hours in culture. This difference was statically significant (p <0.01). In addition, culture of embryos on Matrigel and medium versus medium alone significantly improved in vitro hatching (25.7% versus 3.5%; p <0.01).<br> <strong>Conclusion:</strong>&nbsp;Matrigel at low concentration enhances human blastocyst formation and hatching <em>in vitro</em>. <h1 style="text-align:justify"><br> <span style="font-weight:normal"></span></h1> &nbsp; <h1></h1> Matrigel, Extracellular matrix, Human embryos, Blastocyst, Hatching 103 107 http://ijrm.ir/browse.php?a_code=A-10-549-284&slc_lang=en&sid=1 Marefat Ghaffari Novin mghaffarin@yahoo.com Yes Reproductive Biotechnology Research Centre, Avesina Research Institute, ACECR, Tehran, Iran Mahnaz Heidari No Nanobiotechnology Research Centre, Avesina Research Institute, ACECR, Tehran, Iran Mahdi A Akhondi No Reproductive Biotechnology Research Centre, Avesina Research Institute, ACECR, Tehran, Iran Mahmood Jeddi Tehrani No Monoclonal Antibody Research Center, Avesina Research Institute, ACECR, Tehran, Iran