International Journal of Reproductive BioMedicine
International Journal of Reproductive BioMedicine
IJRM
Medical Sciences
http://ijrm.ir
1
admin
2476-4108
2476-3772
10.29252/ijrm
en
jalali
1395
5
1
gregorian
2016
8
1
14
8
online
1
fulltext
en
اثرات آنتی اکسیدانی و حفاظتی ژل رویال بر تغییرات هیستوپاتولوژی بیضه موش های صحرایی دیابتی
Antioxidant and protective effects of Royal jelly on histopathological changes in testis of diabetic rats
Original Article
Original Article
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مقدمه: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">دیابت شایع­ترین بیماری</span> غدد درون ریز</span> است که اثرات نامطلوبی بر عملکرد دستگاه تناسلی مرد داراست. ژل رویال دارای اثر آنتی اکسیدانی و حفاظتی ضد دیابتی است<strong><span dir="LTR"><span style="font-size:11.0pt;"></span></span></strong><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">هدف: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مطالعه</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> حاضر به منظور ارزیابی اثر</span> ژل رویال</span> بر تغییرات هیستوپاتولوژی از بافت بیضه در موش صحرایی دیابتی شده با استرپتوزوتوسین انجام شد<span dir="LTR"><span style="font-size:11.0pt;">.</span></span><strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"></span></span></strong><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">مواد و روش­ها: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">در این مطالعه تجربی</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، 28</span> موش صحرایی بالغ نژاد ویستار</span> به طور تصادفی به گروه­های کنترل (<span dir="LTR"><span style="font-size:10.0pt;">C</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> ژل رویال (</span></span><span dir="LTR"><span style="font-size:10.0pt;">R</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)،</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> دیابتی (</span></span><span dir="LTR"><span style="font-size:10.0pt;">D</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span>دیابتی درمان شده با ژل­رویال (</span><span dir="LTR"><span style="font-size:10.0pt;">D+R</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) تقسیم شدند.</span> دیابت به وسیله یک تزریق منفرد</span> داخل صفاقی استرپتوزتوسین با دوز <span dir="LTR"><span style="font-size:10.0pt;">mg/kg</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">50 القاء شد.</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> موش­های</span> گروه</span>­های <span dir="LTR"><span style="font-size:11.0pt;">R</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">و </span></span><span dir="LTR"><span style="font-size:10.0pt;">D+R</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">،</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> روزانه </span></span><span dir="LTR"><span style="font-size:10.0pt;">mg/kg</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">100 ژل رویال را به مدت 6 هفته</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> دریافت کرده­اند. رنک </span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">آ</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">میزی</span></span> <span dir="LTR"><span style="font-size:10.0pt;">H&E</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">برای تعیین تغییرات</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> پاتولوژیک بافت بیضه</span> شامل ضخامت</span> تونیکا آلبوژینه (<span dir="LTR"><span style="font-size:10.0pt;">TAT</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)، قطر</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> لوله های منی­ساز (</span></span><span dir="LTR"><span style="font-size:10.0pt;">STsD</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)،معیار جانسون،</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> شاخص های: تمایز لوله­ای (</span></span><span dir="LTR"><span style="font-size:10.0pt;">TDI</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، اسپرماتوژنز (</span></span><span dir="LTR"><span style="font-size:10.0pt;">SPI</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، سلول سرتولی</span> (</span><span dir="LTR"><span style="font-size:10.0pt;">SCI</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)، میوز</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> (</span></span><span dir="LTR"><span style="font-size:10.0pt;">MI</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">و سلول های ایمنی</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> تک هسته</span></span><span dir="LTR"><span style="font-size:11.0pt;">­</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ای (</span></span><span dir="LTR"><span style="font-size:10.0pt;">MIC</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)استفاده شد. وضعیت آنتی اکسیدانی</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> با سنجش سطح ظرفیت تام آنتی­اکسیدان (</span></span><span dir="LTR"><span style="font-size:10.0pt;">FRAP</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">) و کاتالاز (</span></span><span dir="LTR"><span style="font-size:10.0pt;">CAT</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">)مشخص شدند.</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"></span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتایج: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">یافته­های هیستوپاتولوژیک</span></span><strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">موشهای دیابتی،</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> کاهش</span> معنی­داری</span> در شاخص <span dir="LTR"><span style="font-size:10.0pt;">STsD</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">،</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">معیار جانسون</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">،</span></span><span dir="LTR"><span style="font-size:10.0pt;">TDI</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، </span></span><span dir="LTR"><span style="font-size:10.0pt;">SPI</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">، </span></span><span dir="LTR"><span style="font-size:10.0pt;">SCI</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">MI</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و افزایش معنی­دار در </span></span><span dir="LTR"><span style="font-size:10.0pt;">TAT</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:10.0pt;">MIC</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> داشتند، در حالیکه</span></span> تجویز ژل رویال به طور معنی­دار این تغییرات را معکوس نمود (05/0<span style="font-size:11.0pt;">˂</span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">). درمان با ژل رویال به طور قابل توجهی فعالیت</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> آنزیم </span></span><span dir="LTR"><span style="font-size:10.0pt;">CAT</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> و</span></span> <span dir="LTR"><span style="font-size:10.0pt;">FRAP</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> در بافت بیضه</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> را افزایش داد</span></span><span dir="LTR"><span style="font-size:11.0pt;">.</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> اختلاف معنی­داری در سطوح </span></span><span dir="LTR"><span style="font-size:10.0pt;">FRAP</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"> بین گروه­های</span></span><span dir="LTR"><span style="font-size:10.0pt;">C </span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> (5/0</span></span><span style="font-size:10.0pt;">±</span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">13)، </span></span><span dir="LTR"><span style="font-size:10.0pt;">RJ</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> (3/0 </span></span><span style="font-size:10.0pt;">±</span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">4/13)، </span></span><span dir="LTR"><span style="font-size:10.0pt;">D</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> (6/0</span></span><span style="font-size:10.0pt;">±</span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">8/7) و </span></span><span dir="LTR"><span style="font-size:10.0pt;">D+RJ</span></span><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> (07/</span></span><span style="font-size:10.0pt;">±</span><span style="font-family:b mitra;"><span style="font-size:10.0pt;">4/12) وجود دارد</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">(05/0</span></span><span style="font-size:11.0pt;">˂</span><span dir="LTR"><span style="font-size:10.0pt;">p</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;">).</span></span><span style="font-family:b mitra;"><span style="font-size:11.0pt;"></span></span><br>
<strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">نتیجه­ گیری: </span></span></strong><span style="font-family:b mitra;"><span style="font-size:11.0pt;">ژل رویال</span></span> <span style="font-family:b mitra;"><span style="font-size:11.0pt;">اختلات بیضه ی ناشی از دیابت را احتمالاً از طریق خاصیت آنتی­اکسیدانی بهبود می دهد</span></span>.
<strong>Background:</strong> Diabetes is the most common endocrine disease. It has adverse effects on male reproductive function. Royal Jelly (RJ) has antioxidant and anti-diabetic effects and show protective effects against diabetes.<br>
<strong>Objective:</strong> This study was conducted to evaluate the effect of RJ on histopathological alterations of the testicular tissue in streptozotocin (STZ)-induced diabetic rats.<br>
<strong>Materials and Methods: </strong>In this experimental study, 28 adult Wistar rats were randomly divided into control (C), royal jelly (R), diabetic (D) and RJ-treated diabetic (D+R) groups. Diabetes was induced by a single intraperitoneal injection of STZ at 50 mg/kg body weight (BW). The rats from the R and D+R groups received daily RJ (100 mg/kg BW) for 6 wks orally. Hematoxylin-Eosin staining was used to analyze histopathological changes including: tunica albuginea thickness (TAT), seminiferous tubules diameter (STsD), Johnsen’s score, tubular differentiation index (TDI), spermiogenesis index (SPI), Sertoli cell index (SCI), meiotic index (MI), and mononuclear immune cells (MICs) in testes. The antioxidant status was examined by evaluating testicular levels of ferric reducing antioxidant power (FRAP) and catalase (CAT) activity.<br>
<strong>Results:</strong> Histological results of the testis from diabetic rats showed significant decrease in STsD, Johnsen’s score, TDI, SPI, SCI and MI, and significant increase in TAT and MICs, while administration of RJ significantly reverted these changes (p<0.05). RJ treatment markedly increased activity of CAT and FRAP. There were significant differences in FRAP levels among C (13.0±0.5), RJ (13.4±0.3), D (7.8±0.6) and D+R (12.4±0.7) groups (p<0.05).<br>
<strong>Conclusion:</strong> RJ improved diabetes-induced impairment in testis, probably through its antioxidant property.
دیابت ملیتوس, ژل رویال, استرپتوزتوسین, بیضه, هیستوپاتولوژی.
Diabetes mellitus, Royal jelly,Streptozotocin,Testis,Histopathology
519
526
http://ijrm.ir/browse.php?a_code=A-10-1-126&slc_lang=en&sid=1
Elham
Ghanbari
الهام
قنبری
e_ghanbari90@yahoo.com