1 2476-4108 Shahid Sadoughi University of Medical Sciences, Yazd, Iran 1023 Basic and clinical genetic studies on male infertility in Iran during 2000-2016: A review Moghbelinejad Sahar b Mozdarani Hossein c Ghoraeian Pegah d Asadi Reihaneh e b Department of Genetics, School of Sciences, Qazvin University of Medical Sciences, Qazvin, Iran c Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran d Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran e Cellular and Molecular Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran 1 3 2018 16 3 131 148 12 04 2018 12 04 2018 The male factor contributes to 50% of infertility. The cause of male infertility is idiopathic and could be congenital or acquired. Among different factors which are involved in idiopathic male infertility, genetic factors are the most prevalent causes of the disease. Considering, the high prevalence of male infertility in Iran and the importance of genetic factors in the accession of it, in this article we reviewed the various studies which have been published during the last 17 yr on the genetic basis of male infertility in Iran. To do this, the PubMed and Scientific information database (SID) were regarded for the most relevant papers published in the last 17 yr referring to the genetics of male factor infertility using the keywords „„genetics‟‟, “cytogenetic”, „„male infertility”, and “Iranian population”. Literatures showed that among the Iranian infertile men Yq microdeletion and chromosomal aberrations are two main factors that intervene in the genetics of male infertility. Also, protamine deficiency (especially P2) is shown to have an influence on fertilization rate and pregnancy outcomes. The highest rate of sperm DNA damages has been found among the asthenospermia patients. In several papers, the relation between other important factors such as single gene mutations and polymorphisms with male infertility has also been reported. Recognition of the genetic factors that influence the fertility of Iranian men will shed light on the creation of guidelines for the diagnosis, consultation, and treatment of the patients."
1025 The effect of resveratrol on oxidative stress in the liver and serum of a rat model of polycystic ovary syndrome: An experimental study Ghowsi Mahnaz f Khazali Homayoun g Sisakhtnezhad Sajjad h f Department of Animal Physiology, Faculty of Biological Sciences and Technology, Shahid Beheshti University, Tehran, Iran g Department of Animal Physiology, Faculty of Biological Sciences and Technology, Shahid Beheshti University, Tehran, Iran. h Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran 1 3 2018 16 3 149 158 12 04 2018 12 04 2018 Background: Studies of oxidative status in polycystic ovarian syndrome (PCOS) patients are limited with inconsistent results. The effects of resveratrol as a natural antioxidant on oxidative status in PCOS aren’t clear. Objective: This study evaluated effects of resveratrol on oxidative stress in the liver and serum of the PCOS rats. Materials and Methods: Fifteen female Wistar rats (3 wk old) were divided into 3 groups (n=5/each e): Control group, PCO-Control group, and PCO-Resveratrol group. For induction of polycystic ovary phenotype, testosterone enanthate 10 mg/kg was injected for 35 days subcutaneously. Then, resveratrol 10 mg/kg was injected intraperitoneally for 28 days to rats of the PCO-Resveratrol group. Ovarian sections were stained with hematoxylin/eosin. The serum glucose and insulin and the levels of malondialdehyde (MDA) and total antioxidant capacity (TAC) in serum and liver were measured. Results: Control animals showed normal ovarian morphology and PCO-Control animals exhibited cystic follicles. There were no significant differences in liver TAC between groups. The serum MDA (p=0.034), and homeostatic model assessment insulin resistance (HOMA-IR) (p=0.014) levels in PCO-Control rats were higher than the controls. The liver MDA in PCO-Control rats was more than that of controls (p=0.001). The HOMA-IR (p=0.008) and serum MDA (p=0.006) levels in PCO-Control rats were more than those of PCO-Resveratrol rats (p=0.008). In PCO-Resveratrol group, serum TAC was higher than that of PCO-Control group (p=0.022) and liver MDA was more than controls (p=0.01). Conclusion: Results indicated that the induction of PCOS in rats increased lipid peroxidation and insulin resistance and resveratrol improved these complications. 1027 The non-preventive effects of human menopausal gonadotropins on ovarian tissues in Nandrolone decanoate-treated female rats: A histochemical and ultra-structural study Mesbah Fakhroddin i Bordbar Hossein j Talaei Khozani Tahereh k Dehghani Farzaneh l Mirkhani Hossein m i Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran j Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran k Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran l Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran m Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 1 3 2018 16 3 159 174 12 04 2018 12 04 2018 Background: The follicular growth and development may be affected by abused drugs. Nandrolone decanoate (ND) as an anabolic androgenic steroid can damage the morphological and functional features of the ovary and may lead to reproductive failure. Objective: This study was designed to evaluate the effects of synchronized and non-synchronized administration of Human Menopausal Gonadotropins (hMG) with ND on ovarian tissue and level of sex hormones in the adult female rat. Materials and Methods: Forty adult female Sprague Dawley rats were divided into eight groups. The five experimental groups received 3 and/or 10 mg/kg of ND synchronized and non-synchronized with 10 IU of hMG and hMG alone. The two shams and control groups received solvents of ND and hMG. The animals' serum levels of Follicle-stimulating hormone, Luteinizing hormone, progesterone and estrogen and the weight, volume and dimensions of the ovaries were measured. The ovaries were prepared for apoptosis assessment and morphological study. Results: The ovarian volume and sex hormones in the experimental groups were decreased, but ovarian weight and dimensions didn’t change. The rate of apoptosis was increased in the experimental groups as follows; a low and high dose of ND synchronized with hMG 48.80±18.70 and 65.20±14.20 respectively vs. Sham 1, 33.20±17.80, a low and high dose of ND non-synchronized with hMD 55.80±17.20 and 75.20±14.30 respectively vs. Sham 2, 31.60±32.40 groups, p˂0.01. Follicular and stromal cells were damaged in the experimental groups except for the hMG group. Conclusion: Administration of ND decreased the serum level of Luteinizing hormone, Follicle-stimulating hormone, progesterone and estrogen and damaged ovarian tissue irreversibly and irreparably and hMG cannot prevent the destruction of the follicles in the adult female rats. This can be a serious warning to women who abuse ND. 1029 Endogenous luteinizing hormone concentration and IVF outcome during ovarian stimulation in fixed versus flexible GnRH antagonist protocols: An RCT Depalo Raffaella n Trerotoli Paolo o Chincoli Annarosa p Vacca Margherita Patrizia Lamanna Giuseppina Cicinelli Ettore n Department of Obstetrics-Gynecology-Neonatology and Anesthesiology, Unit of Medically Assisted Reproduction and Gametes Cryopreservation, University Hospital of Bari, Bari, Italy. o Department of Biomedical and Human Oncological Science (DIMO), 2nd Unit of Obstetrics and Gynecology, University of Bari, Bari, Italy p Department of Obstetrics-Gynecology-Neonatology and Anesthesiology, Unit of Medically Assisted Reproduction and Gametes Cryopreservation, University Hospital of Bari, Bari, Italy Department of Obstetrics-Gynecology-Neonatology and Anesthesiology, Unit of Medically Assisted Reproduction and Gametes Cryopreservation, University Hospital of Bari, Bari, Italy Consultant Gynaecologist and IVF Specialist, London Women's Clinic, London, UK Department of Obstetrics-Gynecology-Neonatology and Anesthesiology, Unit of Medically Assisted Reproduction and Gametes Cryopreservation, University Hospital of Bari, Bari, Italy 1 3 2018 16 3 175 182 12 04 2018 12 04 2018 Background: Luteinizing hormone (LH) is essential for normal follicular development and oocyte maturation. In particular, fluctuations of LH during the follicular phase have a significant impact on morphological and functional changes of the oocyte and determine its meiotic status and ability to be fertilized. Objective: This prospective randomized controlled trial examined effects of endogenous follicular phase LH levels on oocyte maturity and IVF outcomes in fixed vs. flexible in vitro fertilization. Materials and Methods: Normo-ovulatory women age <39 yr (n=213) were randomized to fixed or flexible gonadotrophin-releasing hormone (GnRH) antagonist protocols. Follicular phase LH, estradiol, and progesterone profiles were measured. Oocytes retrieved, implantation rate, and pregnancy rate were compared between the two groups. Results: LH profiles were similar in both protocols. A lower trend of LH values at the end of ovarian stimulation correlated significantly with a higher pregnancy rate, regardless of protocol (p=0.02). Estradiol levels were statistically different with respect to time points within treatment groups (p<0.0001), but not between groups (p=0.43), or pregnancy outcomes (p=0.2595). Progesterone profiles were similar between groups. No differences were found in retrieved oocytes numbers, fertilization rate or embryos obtained. Significantly, younger age and a higher number of antral follicles were correlated with positive results. Conclusion: Fixed and flexible GnRH antagonist protocols did not produce an oscillation of endogenous LH values correlated to the outcome of ovarian stimulation. 1030 Alteration in CatSper1 and 2 genes expression, sperm parameters and testis histology in varicocelized rats Zohour Soleimani Maryam Jalali Mashayekhi Farideh Mousavi Hasanzade Morteza Baazm Maryam Students Research Committee, Arak University of Medical Sciences, Arak, Iran Department of Biochemistry and Genetics, School of Medicine, Arak University of Medical Sciences, Arak, Iran Students Research Committee, Arak University of Medical Sciences, Arak, Iran Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran. 1 3 2018 16 3 183 190 12 04 2018 12 04 2018 Background: CatSper gene, a member of cation channel sperm family, has an essential role in sperm motility and male fertility. Following varicocele, sperm parameters especially sperm movement decreases. For this reason, we hypothesized that CatSper gene expression might be reduced after varicocele induction in an animal model. Objective: The aim of this study was to evaluate the expression of CatSper 1 and 2 genes, sperm parameters and testis histology following varicocele induction. Materials and Methods: A total of 30 Wistar male rats were randomly divided into three following groups (n=10/ each): control, sham, and varicocele group. Experimental varicocele was induced by partial ligation of the left renal vein. The epididymal sperm parameters, CatSper1 and 2 genes expression, and testes histology were studied two months after varicocele induction. Results: Our results revealed that motility (32.73±16.14%), morphology (48.80±17%) and viability (31.23±9.82%) of sperms significantly reduced following varicocele induction. In addition, we showed a significant decrease in the number of spermatogonia (43.63±5.31) and seminiferous tubules diameters (190.51±19.23 mm) in experimental varicocele rats. The level of CatSper1 and 2 genes expression evaluated using real-time polymerase chain reaction was significantly downregulated 2 months after varicocele induction. Conclusion: Our data indicated that experimental varicocele has deleterious effects on sperm parameters, testis structure as well as the expression of CatSper 1 and 2 genes. 1028 Psychometrics properties of the Iranian version of fertility quality of life tool: A cross- sectional study Hekmatzadeh Seyedeh-Fatemeh Bazarganipour Fatemeh Hosseini Nazafarin Allan Helen Jalali Somayeh Abbasian Zahra Barani Akram Balochi Fereshteh Khademi Saeideh Mahmoudi Tahereh Niknam Roghayeh Khashavi Zahra Taghavi Seyed Abdolvahab Social Determinants of Health Research Center, Yasuj Uneversity of Medical Sciences, Yasuj, Iran Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran Centre for Critical Research in Nursing and Midwifery, School of Health and Education, Middlesex University, London Student Research Committee, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Student Research Committee, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Student Research Committee, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Student Research Committee, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Student Research Committee, Hormozgan University of Medical Sciences, Bandar Abbas, Iran. Student Research Committee, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Student Research Committee, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Hazrate-zahra Infertility Center, Bandar Abbas, Iran Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran 1 3 2018 16 3 191 198 12 04 2018 12 04 2018 Background: Clinical measurement of quality of life (QoL) for assessing reproductive problems should be considered as a standard investigation at the initial and continuing medical consultations with infertile people. Objective: The purpose of this study was comprehensive testing the psychometric properties of the Iranian version of fertility quality of life (FertiQoL). Materials and Methods: This cross-sectional study was conducted on300 women referred to infertility clinic. After linguistic validation, a semi-structured interview was conducted to assess face validity. Consequently exploratory factor analysis was performed to indicate the scale constructs. Discriminate validity was assessed using the known groups comparison. Convergent validity was evaluated by assessing the correlation between similar content on the 12-Item Short Form Health Survey (SF12), Hospital Anxiety and Depression Scale and FertiQol. In addition, reliability analysis was carried out with internal consistency. Results: The reliability of the Iranian version of the FertiQoL was satisfactory in all dimensions (0.77-0.83). Six factors (emotional, mind/body, relational, social, environmental, and tolerability) were extracted from the results of exploratory factor analysis. Discrimination validity showed that FertiQoL can differentiate between female patients with differing duration of infertility and number of children. Moreover, the results of convergent validity showed a favorable correlation between the related dimensions of SF12 (0.43-0.68), Hospital Anxiety and Depression Scale (0.47-0.52) and FertiQoL. Conclusion: The Iranian version of FertiQoL is valid and reliable for assessing infertility problems and the effects of treatment on QoL of infertile patients referred for diagnosis and treatment at infertility clinic. 1026 The effect of supernatant product of adipose tissue derived mesenchymal stem cells and density gradient centrifugation preparation methods on pregnancy in intrauterine insemination cycles: An RCT Fazaeli Hoda Davoodi Faezeh Kalhor Naser Tabatabaii Qomi Reza Research Department, Highly Specialized Jihad Daneshgahi Infertility Treatment Center, Academic Center of Education, Culture and Research (ACECR), Qom, Iran Research Department, Highly Specialized Jihad Daneshgahi Infertility Treatment Center, Academic Center of Education, Culture and Research (ACECR), Qom, Iran Research Department, Highly Specialized Jihad Daneshgahi Infertility Treatment Center, Academic Center of Education, Culture and Research (ACECR), Qom, Iran Research Department, Highly Specialized Jihad Daneshgahi Infertility Treatment Center, Academic Center of Education, Culture and Research (ACECR), Qom, Iran 1 3 2018 16 3 199 208 12 04 2018 12 04 2018 Background: One of the most important involved factors in pregnancy occurrence following intrauterine insemination (IUI) is semen sample preparation. Recently, supernatant product of adipose tissue derived mesenchymal stem cells (SPAS) method has been shown to improve semen parameters. Objective: To compare the effect of preparation methods in order to IUI, SPAS and density gradient centrifugation (DGC). Materials and Methods: This trial was done on 80 couples with male factor infertility who attend jihad daneshgahi infertility treatment center of Qom province, undergoing ovarian stimulation and IUI cycle. Various semen parameters including motility, count, DNA fragmentation and capacitation were evaluated before and after preparation. The effect of semen preparation methods and influence of various semen parameters on pregnancy occurrence were examined. Results: The overall clinical pregnancy rate was 17.5% per patient with no miscarriage. The pregnancy rate for DGC and SPAS were 5% (2 of 40) and 30% (12 of 40) respectively. Since there is no significant difference in improving motion parameters between two groups (except recovery of total number of motile spermatozoa), it seems that these parameters alone are not sufficient to predict IUI pregnancy outcome whereas in samples with >25 million motile spermatozoa in inseminate, there was a clear trend for a higher pregnancy rate for the sample processed using SPAS. Conclusion: Considering SPAS as a new and effective method leading to provide a combination of various improved semen parameters, is expected in near future. 1024 Undesired effect of excessive betamethasone administration during pregnancy: A rare case Mirzaei Alireza Piri Solmaz Gharanizadeh Kaveh Zabihiyeganeh Mozhdeh Bone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, Iran National Association of Iranian Gynecologists and Obstetrician, Tehran, Iran Bone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, Iran Bone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, Iran 1 3 2018 16 3 209 212 12 04 2018 12 04 2018 Background: Postpartum bilateral femoral neck fracture (BFNF) is a rare condition. We here report a case of BFNF due to excessive corticosteroid consumption, twin pregnancy, immobility, and vitamin D deficiency. Case: This is a report of a 32-yr-old woman with bilateral femoral insufficiency fracture five days after emergency cesarean section due to preterm labor, twin pregnancy, and the history of a previous cesarean section at 33 wk. Antenatal repeated courses of betamethasone injections for fetal lung maturity, daily oral use of prednisolone for the history of miscarriage, immobilization, and vitamin D deficiency were the important contributing factors in her past medical history and lab investigations. The bone mineral density examination showed low bone density for the expected age. Conclusion: Clinicians, who deal with pregnant women, should consider the diagnosis of bilateral femoral insufficiency fracture in any pregnant women with pelvic pain. Awareness of risk factors of BFNF might help to reduce the rate of this complication.