2026-10-06T17:07:40+03:30 http://ijrm.ir/browse.php?mag_id=92&slc_lang=en&sid=1

92-820 2026-10-06 10.1002

International Journal of Reproductive BioMedicine IJRM 2476-4108 2476-3772 10.29252/ijrm 2017 15 5 Fatemeh Akyash Fatemeh Sadeghian-Nodoushan Somayyeh Sadat Tahajjodi Habib Nikukar Ehsan Farashahi Yazd Mostafa Azimzadeh Harry D Moore Behrouz Aflatoonian b.aflatoonian@ssu.ac.ir This report explains briefly the minutes of a 1-day workshop entitled; “human embryonic stem cells (hESCs) and good manufacturing practice (GMP)” held by Stem Cell Biology Research Center based in Yazd Reproductive Sciences Institute at Shahid Sadoughi University of Medical Sciences, Yazd, Iran on 27th April 2017. In this workshop, in addition to the practical sessions, Prof. Harry D. Moore from Centre for Stem Cell Biology, University of Sheffield, UK presented the challenges and the importance of the biotechnology of clinical-grade human embryonic stem cells from first derivation to robust defined culture for therapeutic applications Cell Therapy Good Manufacturing Practice Human Embryonic Stem Cell Stem Cells. 2017 6 01 255 256 http://ijrm.ir/article-1-820-en.pdf 10.29252/ijrm.15.5.255

92-827 2026-10-06 10.1002

International Journal of Reproductive BioMedicine IJRM 2476-4108 2476-3772 10.29252/ijrm 2017 15 5 Azita Faramarzi Mohammad Ali Khalili khalili59@hotmail.com Giulietta Micara Azam Agha- Rahimi High implantation success following in vitro fertilization cycles are achieved via thetransfer of embryos with the highest developmental competence. Multiplepregnancies as a result of the transfer of several embryos per cycle accompany withvarious complication. Thus, single-embryo transfer (SET) is the preferred practice inassisted reproductive technique (ART) treatment. In order to improve the pregnancyrate for SET, embryologists need reliable biomarkers to aid their selection ofembryos with the highest developmental potential. Time-lapse technology is anoninvasive alternative conventional microscopic assessment. It providesuninterrupted and continues the survey of embryo development to transfer day.Today, there are four time-lapse systems that are commercially available for ARTcenters. In world and Iran, the first time lapse babies were born in 2010 and 2015,respectively, conceived by SET. Here, we review the use of time-lapse monitoring inthe observation of embryogenesis as well as its role in SET. Although, the findingsfrom our review support common use of time-lapse monitoring in ART centers; but,future large studies assessing this system in well-designed trials are necessary Embryo selection Kinetic Assisted reproductive technology Time-lapse. 2017 6 01 257 264 http://ijrm.ir/article-1-827-en.pdf 10.29252/ijrm.15.5.257

92-826 2026-10-06 10.1002

International Journal of Reproductive BioMedicine IJRM 2476-4108 2476-3772 10.29252/ijrm 2017 15 5 Mohammadreza Gholami Seyyed Amir Yasin Ahmadi Abolfazl Abaszadeh Arash Khaki khaki@iaut.ac.ir Spermatocytogenesis starts from lumens of seminiferous cords and after migrationto the basal membrane ends to the lumens again. We attempt to review the protectiveeffects of melatonin and ghrelin on Spermatocytogenesis and in particular onspermatogonial stem cells, as two rather newly-discovered hormones. Testicularfreezing prior to chemotherapy and radiotherapy is one of the ways of preservingfertility in children with cancer. The freezing has two methods of slow-freezing(cryopreservation) and rapid-freezing (vitrification). Administration of melatonincan maintain the quality of the germ cells underwent such processes, as well asghrelin, can protect germ cells from the toxicities secondary to ischemic injuries,and pathologic apoptosis. This review indicates that in vitro or in vivoadministration of melatonin or ghrelin, could be effective to preserve fertilizationand also they can be used in assisted reproductive technologies to improve thequality of sperms. Future original studies should be propelled toward human studies,of course with observing the ethics. Spermatogonia Cryopreservation Vitrification Melatonin Ghrelin. 2017 6 01 265 272 http://ijrm.ir/article-1-826-en.pdf 10.29252/ijrm.15.5.265

92-825 2026-10-06 10.1002

International Journal of Reproductive BioMedicine IJRM 2476-4108 2476-3772 10.29252/ijrm 2017 15 5 Milad Azami Tayebe Azadi Sepidezahra Farhang Shoobo Rahmati Khadijeh Pourtaghi kh.pourtaghi@yahoo.com Background: Several studies have reported the uncertain role of multi-minerals andvitamins in the prevention of preeclampsia. Objective: The present study aims to investigate the effect of multimineral-vitaminD supplements (calcium, magnesium, zinc and Vitamin D) and vitamins (C+ E) inthe prevention of preeclampsia. Materials and Methods: In this randomized clinical trial, 90 pregnant women weredivided into three groups: group A received Ferrous sulfate (1 tablet/day) + onetablet of Claci-care multimineral-vitamin D containing 800mg calcium, 200mgmagnesium, 8mg zinc and 400 IU Vitamin D3 per day; group B received Ferroussulfate (1 tablet/day) + 250 mg vitamin C + 55 mg vitamin E; and the controlsreceived only one Ferrous sulfate tablet daily. Results: The incidence of preeclampsia in group A was significantly lower than thecontrol group (p=0.03), while there was no significant difference between group Band controls (p=0.50), as well as groups A and B (p=0.063). The incidence ofneonatal complications in the group A was significantly lower than the control group(p=0.01), while there was no significant difference between group B and control(p=0.48). Conclusion: According to the results, calcium, magnesium, and zinc supplementshave a significant effect on the prevention of preeclampsia. In addition, prescriptionof multimineral-vitamin D during pregnancy can be a low-cost and affordable wayto reduce the incidence of preeclampsia in women who are at high risk ofpreeclampsia Prevention Preeclampsia Calcium Magnesium Zinc Vitamin D Vitamin C Vitamin E. 2017 6 01 273 278 http://ijrm.ir/article-1-825-en.pdf 10.29252/ijrm.15.5.273

92-824 2026-10-06 10.1002

International Journal of Reproductive BioMedicine IJRM 2476-4108 2476-3772 10.29252/ijrm 2017 15 5 Parvin Sabeti Fardin Amidi Seyed Mahdi Kalantar Mohammad Ali Sedighi Gilani Soheila Pourmasumi Atefeh Najafi Ali Reza Talebi prof_talebi@ssu.ac.ir Background: Teratoasthenozoospermia (TA) is a severe form of male infertilitywith no clear etiology. Objective: To compare the level of intracellular anion superoxide (O2–), heat shockprotein A2 (HSPA2) and protamine deficiencies in ejaculated spermatozoa betweenteratoasthenozoospermic and normozoospermic men. Materials and Methods: In this case- control study, semen samples of 20 infertilemen, with TA (with normal morphology lower than 4%_ and total motility lowerthan 40% ) as the case group and 20 normozoospermic fertile men as the controlgroup were evaluated for intracellular O2– and HSPA2 by flow cytometry andprotamine deficiency by Chromomycin A3 (CMA3) test. Results: The rate of CMA3+ spermatozoa in the case group was higher thancontrols (p=0.001). The percentages of HSPA2+ spermatozoa in the cases weresignificantly lower than controls (p=0.001). Also, intracellular O2– levels in the casegroup were significantly higher than controls (p=0.001) and had positivecorrelations with sperm apoptosis (r=0.79, p=0.01) and CMA3 positive sperm(r=0.76, p=0.01), but negative correlations with normal morphology (r=-0.81,p=0.01) and motility (r=-0.81, p=0.01). There was no significant correlation betweenintracellular O2– and HSPA2 in the case group (r=0.041, p=0.79). Conclusion: We suggest that the increase in intracellular O2–, decrease inspermatozoa HSPA2+, and high percentages of spermatozoa with immaturechromatin might be considered as etiologies of infertility in TA patients. Male infertility Sperm chromatin HSPA2 Protamine deficiency. 2017 6 01 279 286 http://ijrm.ir/article-1-824-en.pdf 10.29252/ijrm.15.5.279

92-823 2026-10-06 10.1002

International Journal of Reproductive BioMedicine IJRM 2476-4108 2476-3772 10.29252/ijrm 2017 15 5 Payam Amini Saman Maroufizadeh Reza Omani Samani samani@royaninstitute.org Background: The hospital anxiety and depression scale (HADS) is a commonscreening tool designed to measure the level of anxiety and depression in differentfactor structures and has been extensively used in non-psychiatric populations andindividuals experiencing fertility problems. Objective: The aims of this study were to evaluate the factor structure, itemanalyses, and internal consistency of HADS in Iranian infertile patients. Materials and Methods: This cross-sectional study included 651 infertile patients(248 men and 403 women) referred to a referral infertility Center in Tehran, Iranbetween January 2014 and January 2015. Confirmatory factor analysis was used todetermine the underlying factor structure of the HADS among one, two, and threefactormodels. Several goodness of fit indices were utilized such as comparative,normed and goodness of fit indices, Akaike information criterion, and the root meansquared error of approximation. In addition to HADS, the Satisfaction with LifeScale questionnaires as well as demographic and clinical information wereadministered to all patients. Results: The goodness of fit indices through CFAs exposed that three and one-factormodel provided the best and worst fit to the total, male and female datasetscompared to the other factor structure models for the infertile patients. TheCronbach’s alpha for anxiety and depression subscales were 0.866 and 0.753respectively. The HADS subscales significantly correlated with SWLS, indicating anacceptable convergent validity. Conclusion: The HADS was found to be a three-factor structure screeninginstrument in the field of infertility. Anxiety Depression Factor analysis Infertility. 2017 6 01 287 296 http://ijrm.ir/article-1-823-en.pdf 10.29252/ijrm.15.5.287

92-821 2026-10-06 10.1002

International Journal of Reproductive BioMedicine IJRM 2476-4108 2476-3772 10.29252/ijrm 2017 15 5 Farzaneh Fesahat Fatemeh Montazeri Mohammad Hasan Sheikhha Hojjatollah saeedi Razieh Dehghani Firouzabadi Seyed Mehdi Kalantar smkalantar@yahoo.com Background: Selection of the best embryo for transfer is very important in assistedreproductive technology (ART). Using morphological assessment for this selectiondemonstrated that the correlation between embryo morphology and implantationpotential is relatively weak. On the other hand, aneuploidy is a key genetic factorthat can influence human reproductive success in ART. Objective: The aim of this lab trial study was to evaluate the incidence ofaneuploidies in five chromosomes in the morphologically high-quality embryosfrom young patients undergoing ART for sex selection. Materials and Methods: A total of 97 high quality embryos from 23 women at theage of 37or younger years that had previously undergone preimplantation geneticscreening for sex selection were included in this study. After washing, the slides ofblastomeres from embryos of patients were reanalyzed by fluorescence in-situhybridization for chromosomes 13, 18 and 21. Results: There was a significant rate of aneuploidy determination in the embryosusing preimplantation genetic screening for both sex and three evaluated autosomalchromosomes compared to preimplantation genetic screening for only sexchromosomes (62.9% vs. 24.7%, p=0.000). The most frequent detectedchromosomal aneuploidy was trisomy or monosomy of chromosome 13. Conclusion: There is considerable numbers of chromosomal abnormalities inembryos generated in vitro which cause in vitro fertilization failure and it seems thatmorphological characterization of embryos is not a suitable method for choosing theembryos without these abnormalities. Preimplantation genetic screening Aneuploidy Sex chromosome Autosomal 2017 6 01 297 304 http://ijrm.ir/article-1-821-en.pdf 10.29252/ijrm.15.5.297

92-822 2026-10-06 10.1002

International Journal of Reproductive BioMedicine IJRM 2476-4108 2476-3772 10.29252/ijrm 2017 15 5 Mahin Jamshidi Makiani Sara Minaeian sara.minaeian@gmail.com Soheila Amini Moghaddam Seyed Akbar Moosavi Zahra Moeini Vajihe Zamani Mahnaz Karbalaei Sabbagh Hosein Forghani Background: Human papilloma virus (HPV) is one of the major public healthproblems and the main causes of cervical cancer. The prevalence HPV infection indeveloping countries with low financial resources is high. Objective: This study aimed to determine the relative frequency of HPV genotypesand its sociodemographic characteristics in women referred to a general hospital inTehran, Iran from 2014-2015. Materials and Methods: This cross-sectional study was performed in 400 womenwith Pap smear samples, referring to to a general hospital in Tehran, Iran from 2014-2015. The detection of 28 HPV genotypes was performed by using the MultiplexPCR technique. The sociodemographic survey was conducted for each HPV positivewoman. Results: HPV-positive infection was detected in 155 (38.75%) women aged 17-85years. HPV 16 (19.1%) was the most prevalent type, followed by HPV 39 (12.5%)and HPV 18 (8.9%). The highest rate of HPV infection was observed at the age of36 years (7.7%). The level of education and economic situation of each woman wereshowed most of HPV-positive women had a high school diploma (34.6%) andaverage economic situation (67,9%). 60.9% of these women were a housewife, and67.3% lived in the capital . Conclusion: Determination of HPV genotype and risk factor related to HPVinfection in each geographical region can lead to the production of effectivevaccines against the HPV virus. It can also be useful for disease management andhigh sensitivity diagnosis of cervical intraepithelial neoplasia. Human papilloma virus Frequency Multiplex PCR Socioeconomic factors. 2017 6 01 305 310 http://ijrm.ir/article-1-822-en.pdf 10.29252/ijrm.15.5.305