International Journal of
Reproductive Biomedicine
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Volume 19, Issue 3 (March 2021)
IJRM 2021, 19(3): 245-254 |
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Okafor I, Nnamah U S, Ahiatrogah S, Serwaa D, Nnaka J. Reproductive toxicity potentials of Methanolic extract of Portulaca oleracea in male rats: An experimental study. IJRM 2021; 19 (3) :245-254
URL: http://ijrm.ir/article-1-1727-en.html
URL: http://ijrm.ir/article-1-1727-en.html
Izuchukwu Okafor * 
1, Uchenna Somtochukwu Nnamah2 , Selasie Ahiatrogah3 , Dorcas Serwaa3 , Jude Nnaka4
1, Uchenna Somtochukwu Nnamah2 , Selasie Ahiatrogah3 , Dorcas Serwaa3 , Jude Nnaka4
1- Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria. Department of Obstetrics and Gynecology, College of Medicine, University of Ibadan, Ibadan, Nigeria. Pan African University of Life and Earth Science Institute (Including Health and Agriculture), PAULESI, University of Ibadan, Ibadan, Nigeria. , okaforizuchukwu33@gmail.com
2- Hematology Department, Babcock University Teaching Hospital, Ilisan Remo, Ogun State, Nigeria.
3- Department of Obstetrics and Gynecology, College of Medicine, University of Ibadan, Ibadan, Nigeria. Pan African University of Life and Earth Science Institute (Including Health and Agriculture), PAULESI, University of Ibadan, Ibadan, Nigeria.
4- Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria.
2- Hematology Department, Babcock University Teaching Hospital, Ilisan Remo, Ogun State, Nigeria.
3- Department of Obstetrics and Gynecology, College of Medicine, University of Ibadan, Ibadan, Nigeria. Pan African University of Life and Earth Science Institute (Including Health and Agriculture), PAULESI, University of Ibadan, Ibadan, Nigeria.
4- Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria.
Abstract: (1577 Views)
Background: Purslane is an edible widely distributed shrub and one of the herbs used in decoctions for the treatment of different ailments including infertility. However, there is a shortage of evidence to validate its reproductive effects.
Objective: To investigate the effect of methanolic extract of Portulaca oleracea (MEPO) on the reproductive system of male rats.
Materials and Methods: Fifteen 10-wk old male Wistar rats with an average weight of 183 gr were randomly divided into three groups (n = 5/each). Group A (the control group) received distilled water only; group B received 400 mg/kg MEPO; and group C received 800 mg/kg MEPO for 14 days. The animals fasted overnight after the 14th day of administration and euthanized by cervical dislocation. Blood samples, sperm, testes, and epididymis were collected for serum hormones, sperm, and histological analyses.
Results: There was no significant change in the serum luteinizing hormone and testosterone levels across all groups when compared to the control. However, group C showed a significant increase (p = 0.020) in follicle-stimulating hormone levels when compared to the control. There was a significant reduction (p = 0.006) in the sperm count in group C when compared with the control group. There was also a significantly reduced (p = 0.003) sperm motility in MEPO-treated groups compared to the control. While the testis showed no abnormalities in its histoarchitecture across groups, the epididymis showed some blood congestion in MEPO-treated groups.
Conclusion: Portulaca oleracea showed the ability to reduce sperm count and motility at higher doses.
Objective: To investigate the effect of methanolic extract of Portulaca oleracea (MEPO) on the reproductive system of male rats.
Materials and Methods: Fifteen 10-wk old male Wistar rats with an average weight of 183 gr were randomly divided into three groups (n = 5/each). Group A (the control group) received distilled water only; group B received 400 mg/kg MEPO; and group C received 800 mg/kg MEPO for 14 days. The animals fasted overnight after the 14th day of administration and euthanized by cervical dislocation. Blood samples, sperm, testes, and epididymis were collected for serum hormones, sperm, and histological analyses.
Results: There was no significant change in the serum luteinizing hormone and testosterone levels across all groups when compared to the control. However, group C showed a significant increase (p = 0.020) in follicle-stimulating hormone levels when compared to the control. There was a significant reduction (p = 0.006) in the sperm count in group C when compared with the control group. There was also a significantly reduced (p = 0.003) sperm motility in MEPO-treated groups compared to the control. While the testis showed no abnormalities in its histoarchitecture across groups, the epididymis showed some blood congestion in MEPO-treated groups.
Conclusion: Portulaca oleracea showed the ability to reduce sperm count and motility at higher doses.
Type of Study: Original Article |
Subject:
Reproductive Biology
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