Volume 19, Issue 6 (June 2021)                   IJRM 2021, 19(6): 559-568 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Alizadeh M, Nasiri M, Samadi M, ghasemi N, Moradi A. Association of M55L and Q192R polymorphisms of paraoxonase 1 gene (PON1) with recurrent pregnancy loss risk: A case-control study. IJRM 2021; 19 (6) :559-568
URL: http://ijrm.ir/article-1-1864-en.html
Association of M55L and Q192R polymorphisms of paraoxonase 1 gene (PON1) with recurrent pregnancy loss risk: A case-control study
Mehdi Alizadeh1 , Mahboobeh Nasiri2 , Morteza Samadi3 , Nasrin Ghasemi4 , Ali Moradi * 5
1- International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Department of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran.
3- Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
4- Abortion Research Centre, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5- Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran. , morady20018@gmail.com
Abstract:   (1274 Views)
Background: Recurrent pregnancy loss (RPL) refers to the incidence of two or more abortions before the first half of pregnancy. Oxidative stress has been hypothesized to play a central role in RPL.
Objective: To investigate the relationship between Q192R and L55M polymorphisms of PON1 as antioxidant enzyme and the risk of RPL.
Materials and Methods: In this case-control study, 110 women with RPL (case) and 110 healthy fertile women (control) referred to the Research and Clinical Center for Infertility, Shiraz, Iran were enrolled. Genomic DNA was extracted from the peripheral blood in all participants. Polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism method.
Results: Statistical analysis of Q192R polymorphism showed a significant difference for the RR genotype between the case and control group (OR = 11, CI = 1.39-86.87, p = 0.005) but none for the QR and QQ genotypes. No significant association was observed between the R and Q allelic frequency in the RPL participants compared to the control group (p = 0.53). Also, statistical analysis of the L55M polymorphism for MM genotype in the case group compared with the control group showed a significant difference (OR = 3.59, CI = 0.97-13.30, p = 0.042), but none for the LM and LL genotypes.
Conclusion: The findings showed a significant correlation between the Q192R polymorphisms and the L55M PON1 enzyme and RPL in this study population.
Keywords: Pregnancy, Abortion, PON1, Polymorphism, Recurrent pregnancy loss.
Full-Text [PDF 2360 kb]   (575 Downloads) |   |   Full-Text (HTML)  (235 Views)  
Type of Study: Original Article | Subject: Fertility & Infertility
References
1. Niroumanesh S, Mehdipour P, Farajpour A, Darvish S. A cytogenetic study of couples with repeated spontaneous abortions. Ann Saudi Med 2011; 31: 77-79. https://doi.org/10.4103/0256-4947.75785 [DOI:10.5144/0256-4947.2011.77] [PMID] [PMCID]
2. Jeve YB, Davies W. Evidence-based management of recurrent miscarriages. J Hum Reprod Sci 2014; 7: 159-169. [DOI:10.4103/0974-1208.142475] [PMID] [PMCID]
3. Lo W, Rai R, Hameed A, Brailsford SR, Al-Ghamdi AA, Regan L. The effect of body mass index on the outcome of pregnancy in women with recurrent miscarriage. J Family Community Med 2012; 19: 167-171. [DOI:10.4103/2230-8229.102316] [PMID] [PMCID]
4. Branch DW, Gibson M, Silver RM. Clinical practice. Recurrent miscarriage. N Engl J Med 2010; 363: 1740-1747. [DOI:10.1056/NEJMcp1005330] [PMID]
5. Hu X, Miao M, Bai Y, Cheng N, Ren X. Reproductive factors and risk of spontaneous abortion in the jinchang cohort. Int J Environ Res Public Health 2018; 15: 2444. 1-9. [DOI:10.3390/ijerph15112444] [PMID] [PMCID]
6. Agarwal A, Aponte-Mellado A, Premkumar BJ, Shaman A, Gupta S. The effects of oxidative stress on female reproduction: A review. Reprod Biol Endocrinol 2012; 10: 49. 1-31. [DOI:10.1186/1477-7827-10-49] [PMID] [PMCID]
7. Leisegang K, Henkel R, Agarwal A. Redox regulation of fertility in aging male and the role of antioxidants: A savior or stressor. Curr Pharm Des 2017; 23: 4438-4450. [DOI:10.2174/1381612822666161019150241] [PMID]
8. Ramandeep K, Kapil G, Harkiran K. Correlation of enhanced oxidative stress with altered thyroid profile: Probable role in spontaneous abortion. Int J Appl Basic Med Res 2017; 7: 20-25. [DOI:10.4103/2229-516X.198514] [PMID] [PMCID]
9. Lu J, Wang Z, Cao J, Chen Y, Dong Y. A novel and compact review on the role of oxidative stress in female reproduction. Reprod Biol Endocrinol 2018; 16: 80. 1-18. [DOI:10.1186/s12958-018-0391-5] [PMID] [PMCID]
10. Turpin CA, Sakyi SA, Owiredu WKBA, Ephraim RKD, Anto EO. Association between adverse pregnancy outcome and imbalance in angiogenic regulators and oxidative stress biomarkers in gestational hypertension and preeclampsia. BMC Pregnancy Childbirth 2015; 15: 189. 1-10. [DOI:10.1186/s12884-015-0624-y] [PMID] [PMCID]
11. Ghneim HK, Alshebly MM. Biochemical markers of oxidative stress in saudi women with recurrent miscarriage. J Korean Med Sci 2016; 31: 98-105. [DOI:10.3346/jkms.2016.31.1.98] [PMID] [PMCID]
12. Eren SH, Korkmaz I, Guven FMK, Tekin YK, Ozdemir L. Serum paraoxonase, arylesterase, and glutathione-s-transferase activities and oxidative stress levels in patients with mushroom poisoning. Clinics (Sao Paulo) 2018; 73: e16550. [DOI:10.6061/clinics/2018/e16-550] [PMID] [PMCID]
13. Devarajan A, Shih D, Reddy ST. Inflammation, infection, cancer and all that the role of paraoxonases. Adv Exp Med Biol 2014; 824: 33-41. [DOI:10.1007/978-3-319-07320-0_5] [PMID] [PMCID]
14. Shekhanawar M, Shekhanawar SM, Krisnaswamy D, Indumati V, Satishkumar D, Vijay V, et al. The role of 'paraoxonase-1 activity' as an antioxidant in coronary artery diseases. J Clin Diagn Res 2013; 7: 1284-1287. [DOI:10.7860/JCDR/2013/5144.3118] [PMID] [PMCID]
15. Charles-Schoeman C, Lee YY, Shahbazian A, Gorn AH, Fitzgerald J, Ranganath VK, et al. Association of paraoxonase 1 gene polymorphism and enzyme activity with carotid plaque in rheumatoid arthritis. Arthritis Rheum 2013; 65: 2765-2772. [DOI:10.1002/art.38118] [PMID] [PMCID]
16. Bednarz-Misa I, Mierzchala-Pasierb M, Lesnik P, Placzkowska S, Kedzior K, Gamian A, et al. Cardiovascular insufficiency, abdominal sepsis, and patients' age are associated with decreased paraoxonase-1 (PON1) activity in critically Ill patients with multiple organ dysfunction syndrome (MODS). Dis Markers 2019; 2019: 1314623. [DOI:10.1155/2019/1314623] [PMID] [PMCID]
17. Nasiri M, Rasti Z. CTLA-4 and IL-6 gene polymorphisms: Risk factors for recurrent pregnancy loss. Hum Immunol 2016; 77: 1271-1274. [DOI:10.1016/j.humimm.2016.07.236] [PMID]
18. Wang YS, Dai TM, Tian H, Wan FH, Zhang GF. Comparative analysis of eight DNA extraction methods for molecular research in mealybugs. PloS One 2019; 14: e0226818. 1-15. [DOI:10.1371/journal.pone.0226818] [PMID] [PMCID]
19. Hussein YM, Gharib AF, Etewa RL, ElSawy WH. Association of L55M and Q192R polymorphisms in paraoxonase 1 (PON1) gene with breast cancer risk and their clinical significance. Mol Cell Biochem 2011; 351: 117-123. [DOI:10.1007/s11010-011-0718-4] [PMID]
20. Wang S, He G, Chen M, Zuo T, Xu W, Liu X. The role of antioxidant enzymes in the ovaries. Oxid Med Cell Longev 2017; 2017: 4371714. 1-15. [DOI:10.1155/2017/4371714] [PMID] [PMCID]
21. Camps J, Marsillach J, Joven J. The paraoxonases: Role in human diseases and methodological difficulties in measurement. Crit Rev Clin Lab Sci 2009; 46: 83-106. [DOI:10.1080/10408360802610878] [PMID]
22. Chen L, Lu W, Fang L, Xiong H, Wu X, Zhang M, et al. Association between L55M polymorphism in Paraoxonase 1 and cancer risk: A meta-analysis based on 21 studies. Onco Targets Ther 2016; 9: 1151-1158. [DOI:10.2147/OTT.S96990] [PMID] [PMCID]
23. Wang B, Guo Q, Peng Y, Lu J, Singh B, Hua B. Association of AGT M235T and ACE I/D polymorphisms with the risk of ischemic stroke: Meta-analysis in Han Chinese population. J Neurol Sci 2012; 320: 79-84. https://doi.org/10.1016/j.jns.2012.06.022 [DOI:10.1007/s10072-011-0620-6] [PMID]
24. Hernandez-Diaz Y, Tovilla-Zarate CA, Juarez-Rojop IE, Gonzalez-Castro TB, Rodriguez-Perez C, Lopez-Narvaez ML, et al. Effects of paraoxonase 1 gene polymorphisms on heart diseases: Systematic review and meta-analysis of 64 case-control studies. Medicine (Baltimore) 2016; 95: e5298. 1-13. [DOI:10.1097/MD.0000000000005298] [PMID] [PMCID]
25. Gu HF, Mou M, Liang ZG, Sun C, Ren XY, Xiao YB. The association between paraoxonase 1 gene polymorphisms and polycystic ovarian syndrome. Cell Mol Biol 2016; 62: 44-47. [DOI:10.14715/cmb/2016.62.14.7] [PMID]
26. Turgut Cosan D, Colak E, Saydam F, Yazici HU, Degirmenci I, Birdane A, et al. Association of paraoxonase 1 (PON1) gene polymorphisms and concentration with essential hypertension. Clin Exp Hypertens 2016; 38: 602-607. [DOI:10.3109/10641963.2016.1174255] [PMID]
27. Ibrahim AA, El-Lebedy D, Ashmawy I, Hady MA. Association between paraoxonase-1 gene Q192R and L55M polymorphisms in systemic lupus erythematosus (SLE) and anti-phospholipid syndrome (APS) in a population from Cairo of Egypt. Clin Rheumatol 2017; 36: 1305-1310. [DOI:10.1007/s10067-017-3567-z] [PMID]
28. Pappa KI, Gazouli M, Anastasiou E, Loutradis D, Anagnou NP. The Q192R polymorphism of the paraoxonase-1 (PON1) gene is associated with susceptibility to gestational diabetes mellitus in the Greek population. Gynecol Endocrinol 2017; 33: 617-620. [DOI:10.1080/09513590.2017.1302419] [PMID]
29. Motovali-Bashi M, Sedaghat S, Dehghanian F. Association between serum paraoxonase 1 activities (PONase/AREase) and L55M polymorphism in risk of female infertility. Avicenna J Med Biotechnol 2015; 7: 173-178.
30. Mashayekhi F, Behrouzi S, Yousefi M, Salehi Z. The association of PON1 192 Q/R polymorphism and the risk of female infertility. Cell Mol Biol 2015; 61: 74-77.
31. Dadachanji R, Shaikh N, Khavale S, Patil A, Shah N, Mukherjee S. PON1 polymorphisms are associated with polycystic ovary syndrome susceptibility, related traits, and PON1 activity in Indian women with the syndrome. Fertil Steril 2015; 104: 207-216. [DOI:10.1016/j.fertnstert.2015.03.037] [PMID]
32. Mackness MI, Arrol S, Durrington PN. Paraoxonase prevents accumulation of lipoperoxides in low-density lipoprotein. FEBS Lett 1991; 286: 152-154. [DOI:10.1016/0014-5793(91)80962-3]
33. Ozturk E, Pehlivan S, Ozcan C, Ugur MG, Balat O. Paraoxonase-1 polymorphisms (L55M/Q192R) and activities (PONase/AREase) in patients with idiopathic recurrent early pregnancy loss: A preliminary study. Genet Test Mol Biomarkers 2019; 23: 501-505. [DOI:10.1089/gtmb.2018.0245] [PMID]
34. Shunmoogam N, Naidoo P, Chilton R. Paraoxonase (PON)-1: A brief overview on genetics, structure, polymorphisms and clinical relevance. Vasc Health Risk Manag 2018; 14: 137-143. [DOI:10.2147/VHRM.S165173] [PMID] [PMCID]
35. Do not use your browser's 'BACK' button. Use the 'RESET' button to submit more references.
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb