Volume 3, Issue 1 (7-2005)                   IJRM 2005, 3(1): 36-41 | Back to browse issues page

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Rezaei N, Chian R. Effects of essential and non-essential amino acids on in-vitro maturation, fertilization and development of immature bovine oocytes. IJRM 2005; 3 (1) :36-41
URL: http://ijrm.ir/article-1-30-en.html
Effects of essential and non-essential amino acids on in-vitro maturation, fertilization and development of immature bovine oocytes
Nourollah Rezaei * , Ri-Cheng Chian
Abstract:   (3019 Views)
Background: Addition of amino acids to the culture medium is beneficial for embryonic development in many species. Objective: The objective of this study was to investigate the effects of amino acids on the in vitro maturation and embryonic development of the bovine oocyte. Materials and Methods: Bovine ovaries were collected from a local abattoir and brought into laboratory. Cumulus-oocyte complexes (COCs; n=1212) were aspirated from follicles (2-8 mm in diameter) and randomly assigned to four groups for maturation in culture: (1) Basic medium alone as control; (2) Basic medium supplemented with 2% MEM essential amino acids solution; (3) Basic medium supplemented with 1% MEM non-essential amino acids solution; and (4) Basic medium supplemented with 2% MEM essential amino acids solution + 1% MEM non-essential amino acids solution. COCs were incubated in 1 ml maturation medium in an Organ culture dish at 38.5�C in an atmosphere of 5% CO2 with high humidity. After 24 h of culture, 372 oocytes were fixed to determine maturation rate and the remaining oocytes were used for in vitro fertilization (IVF). Following 18 h of insemination, 437 oocytes were fixed and examined for fertilization and 403 oocytes were further cultured. Results: There were no differences in maturation rates and penetration rates among the four groups. Although oocyte cleavage rates were not different in the four groups, embryo development up to the 8-cell stage and blastocyst were significantly higher (p<0.05) in Group (2) and (4) than in the Control and Group (3). Conclusion: These results indicate that the presence of amino acids, especially essential amino acids in the maturation medium is beneficial to oocyte cytoplasmic maturation and subsequent early embryo development in vitro.
Keywords: In vitro maturation, Bovine oocyte, Amino acids
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Type of Study: Original Article |
References
1. Koo DB, kim NH, Lee HT, Chany KS. Effects of fetal calf serum, amino acids, vitamins and insulin on blastocoel formation and hatching of in vivo and lVM/IVF-derived porcine embryos developing in vitro. Theriogenology 1997; 48: 791-802. [DOI:10.1016/S0093-691X(97)00302-6]
2. Lane M, and Gardner DK. Amino acids and vitamins prevent culture-induced metabolic perturbations and associatrd loss of viability of mouse blastocysts. Human Reproduction 1998; 13(4): 991-997. [DOI:10.1093/humrep/13.4.991] [PMID]
3. Swain JE, Bormann CL, Clark SG,Walters EM, Wheeler MB, Krisher RL. Use of energy substrates by various stage preimplantation pig embryos produced in vivo and in vitro. Reproduction 2002; 123(2): 253-260. [DOI:10.1530/rep.0.1230253] [PMID]
4. Anbari K, Schultz RM. Effect of sodium and betaine in culture media on development and relative rates of protein synthesis in preimplantation mouse embryos in vitro. Mol Reprod Dev 1993; 35: 24-28. [DOI:10.1002/mrd.1080350105] [PMID]
5. Edwards LJ, Williams DA, Gardner DK. Intracellular PH of the mouse preimplantation embryo: amino acids act as buffers of intracellular PH. Hum Reprod 1998;13: 344-348. [DOI:10.1093/humrep/13.12.3441] [PMID]
6. Bavister BD. Culture of preimplantation embryos: facts and artifacts. Hum Reprod update 1995; 1:91-148. [DOI:10.1093/humupd/1.2.91] [PMID]
7. Bae IH, and Foote RH. Carbohydrate and amino acid requirements and ammonia production of rabbit follicular oocytes matured in vitro. Exp Cell Res1975; 91(1): 113-118. [DOI:10.1016/0014-4827(75)90148-2]
8. Gwatkin and Haidri. Requirements for the maturation of hamster oocytes in vitro. Exp Cell Res 1973; 76(1): 1-7. [DOI:10.1016/0014-4827(73)90411-4]
9. Ka HH, Sawai K, Wang WH, Im KS, Niwa K. Amino acids in maturation medium and presence of cumulus cells at fertilization promote male pronuclear formation in porcine oocytes matured and penetrated in vitro. Biol Reprod1997; 57(6): 1478-1483. [DOI:10.1095/biolreprod57.6.1478] [PMID]
10. Rose-Hellekant TA, Libersky-Williamson E.A. Bavister B.D: Energy substrates and amino acids provided during in vitro maturation of bovine oocytyes alter acquisition of developmental competence. Zygote 1998; 60: 285-294. [DOI:10.1017/S0967199498000239] [PMID]
11. Chian RC, Chung J, Downey BR, Tan SL. Maturational and developmental competence of immature oocytes retrieved from bovine ovaries at different phases of folliculogenesis. Reproductive Biomedicine Online 2002; 4(2): 129-134. [DOI:10.1016/S1472-6483(10)61929-3]
12. Downs SM, and Mastropolo AM. Culture conditions affect meiotic regulation in cumulus-cell enclosed mouse oocytes. Mol Reprod Dev 1997; 46: 551-566. https://doi.org/10.1002/(SICI)1098-2795(199704)46:4<551::AID-MRD13>3.0.CO;2-Z [DOI:10.1002/(SICI)1098-2795(199704)46:43.0.CO;2-Z]
13. Kito S, and Bavister BD. Maturation of hamster oocytes under chemically defined conditions and sperm penetration through the zona pelucida. Zygote 1997; 4: 199-210. [DOI:10.1017/S0967199400003117] [PMID]
14. Hardy K, Wright CS, Franks S, Winston RML. In vitro maturation of oocytes. British Medical Bulletin 2000; 56(3): 588-602. [DOI:10.1258/0007142001903391] [PMID]
15. Lim Jm, Lee BC, Lee EC, Chung HM, Koj J, Paik SE, et al. In vitro maturation and in vitro Fertilization of bovine oocytes cultured in a chemichally defined, protein free medium: effects of carbohydrates and amino acids. Reprod Fertil Dev 1999; 11(2): 127-132. [DOI:10.1071/RD99001] [PMID]
16. Downs SM, and Hudson ED. Energy substrates and the completion of spontaneous meiotic maturation. Zygote 2000; 8: 339-351. [DOI:10.1017/S0967199400001131] [PMID]
17. Krisher RL, Bavister BD. Enhanced glycolysis after maturation of bovine oocytes in vitro is associated with increased developmental competence. Mol Reprod Dev 1999; 53(1): 19-26. https://doi.org/10.1002/(SICI)1098-2795(199905)53:1<19::AID-MRD3>3.0.CO;2-U [DOI:10.1002/(SICI)1098-2795(199905)53:13.0.CO;2-U]
18. Zheng p, Bavister BD, Ji WZ. Amino acid requirements for muturation of rhesus monkey (Mac acca mulatta) oocytes in culture. Reproduction 2002; 124: 515-522. [DOI:10.1530/rep.0.1240515] [PMID]
19. Hong JY, Yong HY, Lee BC, Hwang WS, Lim JM, Lee ES. Effects of amino acids on maturation, fertilization and embryo development of pig follicular oocytes in two IVM media. Theriogenology 2004; 62(8): 1473-1482. [DOI:10.1016/j.theriogenology.2004.02.013] [PMID]
20. Li J, and Foote KH. Effect of inositol with increasing sodium chloride and constant osmolarity on cultured rabbit embryos. J Assisted Reprod Genetics 1995; 12: 141-146. [DOI:10.1007/BF02211384] [PMID]
21. Bavister BD, Leibfried ML, Leiberman G. Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol Reprod 1983; 28(1): 235-247. [DOI:10.1095/biolreprod28.1.235] [PMID]
22. Corney EW, and Bavister BD. Stimulatory and inhibitory effects of amino acids on the development of hamster 8-cell embryo in vitro. J In Vitro Fertile and Embryo Transfer 1987; 4: 162-167. [DOI:10.1007/BF01555464] [PMID]
23. Gardner DK, and Lane M. Amino acids and ammonium regulate mouse embryo development in culture. Biol Reprod 1993; 48: 377-385. [DOI:10.1095/biolreprod48.2.377] [PMID]
24. Gardner DK, Lane M, Spitzer A, Batt P. Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells, amino acids, vitamins and culturiny embryos in groups stimulate development. Biol Reprod 1994; 50: 390-400. [DOI:10.1095/biolreprod50.2.390] [PMID]
25. Liu z, and Foote RH. Effects of amino acids on the development of in vitro maturation in vitro fertilization bovine embryos in a simple protein-free medium. Human Reproduction 1995; 10(ll): 2925-2991. [DOI:10.1093/oxfordjournals.humrep.a135834] [PMID]
26. Elhassan YM, Wu G, Leanez AC, Tasca RJ, Watson AJ, Westhusin ME. Amino acid concentrations in fluids from the bovine oviduct and uterus and in ksom-based culture media. Theriogenology 2001; 55:1907-1918. [DOI:10.1016/S0093-691X(01)00532-5]
27. Van Winkle LJ. Amino acid transport regulation and early embryo development. Biology of Reproduction 2001; 64: 1-12. [DOI:10.1095/biolreprod64.1.1] [PMID]
28. Liu Zishu, Foote Robert H, simkin Michael E. Effect of amino acids and α-Amanitin on the development of Rabbit Embryos in Modified Protein-Free KSOM with Hepes. Molecular Reproduction and development 1996; 45: 157-162. https://doi.org/10.1002/(SICI)1098-2795(199610)45:2<157::AID-MRD8>3.0.CO;2-S [DOI:10.1002/(SICI)1098-2795(199610)45:23.0.CO;2-S]
29. Bolten VN, Oades PJ, Johnson MH. The relationship between cleavage, DNA replication, and gene expression in the mouse 2-cell embryo. J Emoryol EXP Morphol 1984; 75: 139-169.
30. Steeves TE, and Gardner DK. Temporal and differential effects of amino acids on bovine embryo development in culture. Biology of Reproduction GL 1999; 61(3): 731-740. [DOI:10.1095/biolreprod61.3.731] [PMID]
31. Watson AJ, De Sousa P, Eaveneyn A, Barcroft LC, Natale D, Urguhard J, et al. Impact of bovine oocyte maturation media on oocyte transcript levels, blastocyst development, cell number and apoptosis. Biol Reprod 2000; 62: 353-364. [DOI:10.1095/biolreprod62.2.355] [PMID]
32. Schultz GA, Kaye PL, Mckay DJ, Johnson MH. Endogenous amino acid pool size in mouse eggs and preimplantation embryos. J Reprod Fertil 1981; 61: 387-393. [DOI:10.1530/jrf.0.0610387] [PMID]
33. Pinyopummintr T, and Bavister BD. Effects of amino acids on development in vitro of cleavage stage bovine embryos into blastocysts. Reprod Fertil Dev 1996; 8: 835-841. [DOI:10.1071/RD9960835] [PMID]
34. Lane M, and Gardner DK. Nonessential amino acids and glutamine decrease time of the first three cleavage divisions and increase compaction of mouse zygotes in vitro. J Assist Reprod Genet 1997a; 14(7): 398- 403. [DOI:10.1007/BF02766148] [PMID] [PMCID]
35. Kim JH, Niwa k, Lim JM, Okuda k. Effects of Phosphute, Energy Substrates and amino acids on development of in vitro matured, in vitro fertilized bovine oocytes in a chemically defined, protein free culture medium. Biol Reprod 1993; 48: 1320-1325. [DOI:10.1095/biolreprod48.6.1320] [PMID]
36. Lane M, and Gardner DK. Differential regulation of mouse embryo development and viability by amino acids. J Reprod Fertil Dev 1997 b; 109: 153-164. [DOI:10.1530/jrf.0.1090153] [PMID]
37. Rosenkrans CF Jr, Dans DL, Milliken G. Pig blastocyst development in vitro is affected by amino acids. J Anim Sci 1993; 67: 1503-1508. [DOI:10.2527/jas1989.6761503x] [PMID]
38. Bavister BD, McKiernan SH. Regulation of hamster embryo development in vitro by amino acids. In: Bavister BD (ed.), Preimplantation Embryo Development. New York: Springer-Verlag; 1993: 57-72. [DOI:10.1007/978-1-4613-9317-7_5]
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